A5014270374- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Developmental Biology and Gene Regulation
- Digestive system and related health
- Congenital heart defects research
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- Immune Cell Function and Interaction
- Renal and related cancers
- Gut microbiota and health
- Cytokine Signaling Pathways and Interactions
- Immunotherapy and Immune Responses
- IL-33, ST2, and ILC Pathways
- Neurogenetic and Muscular Disorders Research
- Genetics and Neurodevelopmental Disorders
- Axon Guidance and Neuronal Signaling
- Reinforcement Learning in Robotics
- Congenital gastrointestinal and neural anomalies
- Thyroid Cancer Diagnosis and Treatment
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Congenital limb and hand anomalies
- Hippo pathway signaling and YAP/TAZ
- Invertebrate Immune Response Mechanisms
- Inflammasome and immune disorders
- Cell death mechanisms and regulation
University Medical Center of the Johannes Gutenberg University Mainz
2019-2023
Johannes Gutenberg University Mainz
2019-2023
Institute of Molecular Biology
2013-2018
Institute of Gene Biology
2017
University of Geneva
2008-2014
École Polytechnique Fédérale de Lausanne
2013
Max Delbrück Center
2003-2006
The gut microbiota influences intestinal barrier integrity through mechanisms that are incompletely understood. Here we show the commensal weakens by suppressing epithelial neuropilin-1 (NRP1) and Hedgehog (Hh) signaling. Microbial colonization of germ-free mice dampens signaling Hh pathway Toll-like receptor (TLR)-2, resulting in decreased NRP1 protein levels. Following activation via TLR2/TLR6, NRP1, a positive-feedback regulator signaling, is lysosomally degraded. Conversely, elevated...
By conditional gene ablation in mice, we found that β-catenin, an essential downstream effector of canonical Wnt signaling, is a key regulator formation the apical ectodermal ridge (AER) and dorsal–ventral axis limbs. generation compound mutants, also show β-catenin acts BMP receptor IA AER induction, but upstream or parallel patterning. Thus, patterningof limbs are tightly controlled by intricate interplay between Wnt/β-catenin signaling.
Epigenetic mechanisms, including chromatin structure, dynamics and histone modifications play an important role for maintenance differentiation of pluripotent embryonic stem cells. However, little is known about the molecular mechanisms adult cell specification differentiation. Here, we used intestinal cells (ISCs) as a model system to reveal epigenetic changes coordinating gene expression programs during these processes. We found that two distinct participate in establishing transcriptional...
Polycomb group (PcG) proteins are essential for the repression of key factors during early development. In Drosophila, polycomb repressive complexes (PRC) associate with defined response DNA elements (PREs). mammals, however, mechanisms underlying recruitment at targeted loci poorly understood. We have used an in vivo approach to identify sequences importance proper HoxD locus. report that various genomic re-arrangements gene cluster do not strongly affect PRC2 and relatively small...
The search for new immune response mechanisms capable of controlling immune-evasive tumor cells devoid the MHC antigen is a challenging task immunologists. In this study, we found that treatment human peripheral blood lymphocytes with innate immunity protein Tag7 (PGRP-S, PGLYRP1) induces differentiation populations NK (natural killer) and CD8+ CD4+ T are cytotoxic leukocyte antigen-negative cells. These employ different cell lysis (based on release granzymes in case FasL-Fas interaction...
AIM2 is an interferon-inducible HIN-200 protein family member and well-documented for its roles in innate immune responses as a DNA sensor. Recent studies have highlighted AIM2's function on regulatory T cells (Treg) follicular (Tfh). However, involvement Th17 cell differentiation remains unclear. This study reveals that promotes differentiation. deficiency decreases IL-17A production downregulates key associated proteins (RORγt, IL-1R1, IL-23R). located the nucleus of cells, where it...
ABSTRACT An important problem in cellular immunology is to identify new populations of cytotoxic lymphocytes capable killing tumor cells that have lost classical components MHC‐machinery and understand mechanisms the death these cells. We previously found CD4 + CD25 appear lymphokine‐activated killer (LAK) cell culture, which carry Tag7 (PGRP‐S) FasL proteins on their surface can kill Hsp70‐ Fas‐expressing HLA‐negative In this work, we continued study cells, focusing time CD8 lymphocytes....
The mammalian gut is the largest endocrine organ. Dozens of hormones secreted by enteroendocrine cells regulate a variety physiological functions but also pancreas and brain. Here, we examined role helix-loop-helix transcription factor ID2 during differentiation intestinal stem along lineage.To assess in adult mouse small intestine, used single-cell RNA sequencing, genetically modified mice, organoid assays.We found that epithelium Id2 predominantly expressed enterochromaffin peptidergic...
The identity of embryonic gastric epithelial progenitors is unknown. We used single-cell RNA sequencing, genetic lineage tracing and organoid assays to assess whether Axin2 Lgr5 expressing cells are in the developing mouse stomach. show that Axin2+ represent a transient population forestomach. Lgr5+ generate both glandular corpus squamous forestomach organoids ex vivo. Only give rise zymogenic culture. Modulating activity WNT, BMP Notch pathways vivo vivo, we found WNTs essential for...
Continuous and rapid renewal of the intestinal epithelium depends on stem cells (ISCs). A large repertoire transcription factors mediates correct maintenance differentiation ISCs along either absorptive or secretory lineages. In present study, we addressed role TCF7L1, a negative regulator WNT signalling, in embryonic adult using conditional mouse mutants. We found that TCF7L1 prevents precocious epithelial progenitors towards enterocytes ISCs. show Tcf7l1 deficiency leads to upregulation...