A5021048991- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Liver Disease Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Bioinformatics and Genomic Networks
- Genetic Associations and Epidemiology
- Metabolism and Genetic Disorders
- Metabolism, Diabetes, and Cancer
- Birth, Development, and Health
- Hormonal Regulation and Hypertension
- Nutritional Studies and Diet
- Advanced Proteomics Techniques and Applications
- Pancreatic function and diabetes
- Tryptophan and brain disorders
- Hormonal and reproductive studies
- Mitochondrial Function and Pathology
- Gestational Diabetes Research and Management
- Gut microbiota and health
- Ruminant Nutrition and Digestive Physiology
- Fatty Acid Research and Health
- Amino Acid Enzymes and Metabolism
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Reproductive Physiology in Livestock
Helmholtz Zentrum München
2016-2025
Helmholtz Association of German Research Centres
2023
Center for Environmental Health
2012-2023
Institut Gustave Roussy
2020
Imperial College London
2020
Deutsches Diabetes-Zentrum e.V.
2014-2018
German Center for Diabetes Research
2014-2018
Heinrich Heine University Düsseldorf
2014-2018
German Institute of Human Nutrition
2014-2016
Charité - Universitätsmedizin Berlin
2016
Metabolomic discovery of biomarkers type 2 diabetes (T2D) risk may reveal etiological pathways and help to identify individuals at for disease. We prospectively investigated the association between serum metabolites measured by targeted metabolomics T2D in European Prospective Investigation into Cancer Nutrition (EPIC)-Potsdam (27,548 adults) among all incident cases (n = 800, mean follow-up 7 years) a randomly drawn subcohort 2,282). Flow injection analysis tandem mass spectrometry was used...
Article25 September 2012Open Access Novel biomarkers for pre-diabetes identified by metabolomics Rui Wang-Sattler Corresponding Author Research Unit of Molecular Epidemiology, Helmholtz Zentrum München, Neuherberg, Germany Search more papers this author Zhonghao Yu Christian Herder German Diabetes Center, Institute Clinical Diabetology, Leibniz Center at Heinrich Heine University, Düsseldorf, Ana C Messias Structural Biology, Anna Floegel Department Human Nutrition Potsdam-Rehbruecke,...
Background Human plasma and serum are widely used matrices in clinical biological studies. However, different collecting procedures the coagulation cascade influence concentrations of both proteins metabolites these matrices. The effects on metabolite concentration profiles have not been fully characterized. Methodology/Principal Findings We analyzed 163 samples collected simultaneously from 377 fasting individuals. To ensure data quality, 41 with low measurement stability were excluded...
Metabolomic profiling and the integration of whole-genome genetic association data has proven to be a powerful tool comprehensively explore gene regulatory networks investigate effects variation at molecular level. Serum metabolite concentrations allow direct readout biological processes, specific metabolomic signatures with complex diseases such as Alzheimer's disease cardiovascular metabolic disorders been shown. There are well-known correlations between sex incidence, prevalence, age...
Aging is a major risk factor for large number of disorders and functional impairments. Therapeutic targeting the aging process may therefore represent an innovative strategy in quest novel broadly effective treatments against age-related diseases. The recent report lifespan extension mice treated with FDA-approved mTOR inhibitor rapamycin represented first demonstration pharmacological maximal mammals. Longevity effects may, however, be due to rapamycin’s on specific life-limiting...
Summary Understanding the complexity of aging is utmost importance. This can now be addressed by novel and powerful approach metabolomics. However, to date, only a few metabolic studies based on large samples are available. Here, we provide specific information age‐related metabolite concentration changes in human homeostasis. We report results from two population‐based studies: KORA F4 study Germany as discovery cohort, with 1038 female 1124 male participants (32–81 years), TwinsUK...
Metabolic challenge protocols, such as the oral glucose tolerance test, can uncover early alterations in metabolism preceding chronic diseases. Nevertheless, most metabolomics data accessible today reflect fasting state. To analyze dynamics of human metabolome response to environmental stimuli, we submitted 15 young healthy male volunteers a highly controlled 4 d protocol, including 36 h fasting, and lipid tests, liquid test meals, physical exercise, cold stress. Blood, urine, exhaled air,...
A critical question facing the field of metabolomics is whether data obtained from different centers can be effectively compared and combined. An important aspect this interlaboratory precision (reproducibility) analytical protocols used. We analyzed human samples in six laboratories using instrumentation but a common protocol (the AbsoluteIDQ p180 kit) for measurement 189 metabolites via liquid chromatography (LC) or flow injection analysis (FIA) coupled to tandem mass spectrometry (MS/MS)....
Background Information regarding the variability of metabolite levels over time in an individual is required to estimate reproducibility measurements. In intervention studies, it critical appropriately judge changes that are elicited by any kind intervention. The pre-analytic phase (collection, transport and sample processing) a particularly important component data quality multi-center studies. Methods Reliability metabolites (within-and between-person variance, intraclass correlation...
Objectives— To determine whether the level of lysophosphatidylcholine (lysoPC) generated by lipoprotein-associated phospholipase A2 (Lp-PLA2) is associated with severity inflammation in human atherosclerotic plaques. Elevated plasma Lp-PLA2 increased cardiovascular risk. inhibition reduces atherosclerosis. hydrolyzes low-density lipoprotein–oxidized phospholipids generating lysoPCs. According to vitro studies, lysoPCs are proinflammatory but association between their generation and plaque...
<b><i>Objective: </i></b>The human serum metabolite profile is reflective of metabolic processes, including pathophysiological changes characteristic diseases. Therefore, investigation concentrations in obese children might give new insights into biological mechanisms associated with childhood obesity. <b><i>Methods: </i></b>Serum samples 80 and 40 normal-weight between 6 15 years age were analyzed using a mass spectrometry-based metabolomics...
Schizophrenia is a severe complex mental disorder affecting 0.5–1% of the world population. To date, diagnosis disease mainly based on personal and thus subjective interviews. The underlying molecular mechanism schizophrenia poorly understood. Using targeted metabolomics we quantified compared 103 metabolites in plasma samples from 216 healthy controls 265 schizophrenic patients, including 52 cases that do not take antipsychotic medication. Compared with controls, levels five were found...
Identification and treatment of the rupture prone atherosclerotic plaque remains a challenge for reducing burden cardiovascular disease. The interconnection metabolic inflammatory processes in plaques is poorly understood. Herein, we investigate associations between metabolite profiles, mediators vulnerability carotid plaques.We collected 159 from patients undergoing endarterectomy measured 165 different metabolites targeted metabolomics approach. We identified profile that associated with...
Prolonged storage of biospecimen can lead to artificially altered metabolite concentrations and thus bias data analysis in metabolomics experiments. To elucidate the potential impact long-term on profile, a pooled human plasma sample was aliquoted stored at −80 °C. During time period five years, 1012 aliquots were measured with Biocrates AbsoluteIDQ p180 targeted-metabolomics assay 193 points. Modeling concentration courses over revealed that 55 out 111 metabolites remained stable. The...
Serum metabolites are associated cross-sectionally with kidney function in population-based studies. Using flow injection and liquid chromatography tandem mass spectrometry methods, we examined longitudinal associations of baseline concentrations 140 their 19 460 ratios decline chronic disease (CKD) incidence over 7 years 1104 participants the Cooperative Health Research Region Augsburg S4/F4 study. Corrected for multiple testing, a significant association annual change estimated glomerular...
Abstract Background Metabolomics helps to identify links between environmental exposures and intermediate biomarkers of disturbed pathways. We previously reported variations in phosphatidylcholines male smokers compared with non-smokers a cross-sectional pilot study small sample size, but knowledge the reversibility smoking effects on metabolite profiles is limited. Here, we extend our metabolomics large prospective including female quitters. Methods Using targeted approach, quantified 140...
Metabolomics is a promising tool for discovery of novel biomarkers chronic disease risk in prospective epidemiologic studies. We investigated the between- and within-person variation concentrations 163 serum metabolites over period 4 months to evaluate metabolite reliability expressed by intraclass-correlation coefficient (ICC: ratio between-person variance total variance). The analyses were performed with BIOCRATES AbsoluteIDQ™ targeted metabolomics technology, including acylcarnitines,...
Nutrition plays an important role in human metabolism and health. Metabolomics is a promising tool for clinical, genetic nutritional studies. A key question to what extent metabolomic profiles reflect patterns epidemiological setting. We assessed the relationship between intake women from large cross-sectional community study. Food frequency questionnaires (FFQs) were applied 1,003 TwinsUK cohort with targeted analyses of serum samples using Biocrates Absolute-IDQ™ Kit p150 (163...