A5101530491- Protein Structure and Dynamics
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Pulsed Power Technology Applications
- Microbial Inactivation Methods
- DNA and Nucleic Acid Chemistry
- Photosynthetic Processes and Mechanisms
- Metalloenzymes and iron-sulfur proteins
- Plasma Applications and Diagnostics
- Gyrotron and Vacuum Electronics Research
- Electrostatic Discharge in Electronics
- Genomics and Phylogenetic Studies
- Food Drying and Modeling
- Chemical Synthesis and Analysis
- Plant Micronutrient Interactions and Effects
- Dupuytren's Contracture and Treatments
- Transportation Planning and Optimization
- Hemoglobin structure and function
- Nail Diseases and Treatments
- Pharmacogenetics and Drug Metabolism
- Advanced Proteomics Techniques and Applications
- Maritime Ports and Logistics
- Radioactive element chemistry and processing
- Metabolomics and Mass Spectrometry Studies
- Metal-Catalyzed Oxygenation Mechanisms
Instituto Politécnico de Lisboa
2011-2017
University of Lisbon
2012-2016
Universidade Nova de Lisboa
1995-2012
Center for Integrated Protein Science Munich
2011
Technical University of Munich
2011
Helmholtz Zentrum München
2011
Hôpital Dupuytren
2006
European Molecular Biology Laboratory
2002
Instituto de Biologia Experimental e Tecnológica
1999
Background Human plasma and serum are widely used matrices in clinical biological studies. However, different collecting procedures the coagulation cascade influence concentrations of both proteins metabolites these matrices. The effects on metabolite concentration profiles have not been fully characterized. Methodology/Principal Findings We analyzed 163 samples collected simultaneously from 377 fasting individuals. To ensure data quality, 41 with low measurement stability were excluded...
O frênulo lingual é uma prega fibrosa que conecta a língua ao assoalho da boca. Quando este restritivo, ocorre anquiloglossia. presente estudo tem como objetivo revisar, analisar e reunir as evidências atuais sobre o impacto anquiloglossia, importância de seu diagnóstico precoce necessidade do acompanhamento adequado para estabelecer melhor tratamento. Trata-se revisão literatura, caráter qualitativo abordagem descritiva. Foram identificados 29 artigos temática publicados nos últimos 5 anos,...
The aim of this study was to report on a dental intervention using combination two therapeutic approaches, the manual scalpel and laser technique, associated with local photobiomodulation Low Level Laser Therapy (LLLT), in order reduce an episode severe gingival hyperplasia continuous use anticonvulsants drugs patients neurological disorders. A 15-year-old male clinical history hydrocephalus bedridden limited movement interaction deficit epilepsy, admitted intensive care unit charitable...
Side-chain modeling has a widespread application in many current methods for protein tertiary structure determination, prediction, and design. Of the existing side-chain methods, rotamer-based are fastest most efficient. Classically, rotamer is conceived as single, rigid conformation of an amino acid side-chain. Here, we present flexible model which continuous ensemble conformations that cluster around classic rotamer. We have developed thermodynamically based method calculating effective...
A seven‐iron ferredoxin was isolated from aerobically grown cells of the hyperthermoacidophilic archaeon Desulfurolobus ambivalens (DSM 3772). The protein is monomeric, with an apparent molecular mass 15 kDa and contains 7 iron atoms/molecule. N‐terminal sequence shows a large similarity (70% identity) that Sulfolobus acidocaldarius. EPR characteristics in both native (oxidized) dithionite‐reduced states this allowed unequivocal identification [3Fe‐4S] 1+/0 center, reduction potential −270 ±...
With the objective of improving side-chain conformation prediction, we have analyzed influence various factors on prediction by Self-Consistent Mean Field Theory method, applied to a set high resolution x-ray protein structure models. These may be classed as variations in mean field optimization protocol, potential energy function, and rotamer library completeness. We developed an protocol that consistently reached lower conformational free energies than two other protocols. This led...
The effect of monopolar and bipolar shaped pulses in additional yield apple juice extraction is evaluated. applied electric field strength, pulsewidth, number are assessed for both pulse types, divergences analyzed. Variation strength ranged from 100 to 1300 V/cm, pulsewidth 20 300 μs, the 10 200, at a frequency 200 Hz. Two trains separated by 1 s cubes. Results plotted against reference untreated samples all assays. Specific energy consumption calculated each experiment as well qualitative...
Sub-nanosecond bipolar high voltage pulses are a very important tool for food processing, medical treatment, waste water and exhaust gas processing. A Hybrid Modulator sub-microsecond pulse generation, comprising an unipolar solidstate Marx generator connected to load through stack Blumlein system that produces further multiplies the amplitude, is presented. Experimental results from assembled prototype show generation of 1000 V amplitude with 100 ns width 1 kHz repetition rate.
Correct alignment of the sequence a target protein with those homologues known three-dimensional structure is key step in comparative modeling. Usually an iterative approach that takes account local and overall structural features required. We describe such exploits databases alignments homologous proteins (HOMSTRAD, http:/(/)www-cryst.bioc.cam.ac.uk/ approximately homstrad) superfamilies (CAMPASS, campass), which structure-based are analyzed formatted program JOY...
A dynamic model for marginal cost pricing of port infrastructures links costs to system performance by combining a power-law function with time-dependent queueing analysis. Additionally, the incorporates capacity, including effects economies scale. This allows calculation price under framework. The accounts nonlinear behaviour demand, which is sensitive and service levels. over time levels on port's operational are quantified. proposed determining optimal timing capacity investment. starting...
The seven-iron ferredoxin from the hyperthermophilic archaeon Desulfurolobus ambivalens has been investigated by one-dimensional and two-dimensional 1H-NMR in its oxidized dithionite-reduced states. All iron atoms of both three-iron four-iron cluster are bound to cysteine residues whose hyperfine-shifted resonances were characterized. pattern these is similar those three-iron, eight-iron ferredoxins previously described literature, but a shift different that other proteins. A second set...
The performance of the self-consistent mean field theory (SCMFT) method for side-chain modeling, employing rotamer energies calculated with flexible model (FRM), is evaluated in context comparative modeling protein structure. Predictions were carried out on a test set 56 backbones varying accuracy, to allow prediction accuracy be analyzed as function backbone accuracy. A progressive decrease was observed decreased. However, even very low substantially higher than random, indicating that FRM...
This paper models an <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">n</i> -stage stacked Blumlein generator for bipolar pulses various load conditions. Calculation of the voltage amplitudes in time domain at and between stages is described generator. For this, reflection transmission coefficients are mathematically modeled where impedance discontinuity occurs (i.e., junctions two lines). The mathematical model developed assessed by comparing...
Desde o aleitamento materno exclusivo até a alimentação sólida, nutrição na infância se faz essencial para desenvolvimento adequado da criança. Nesse sentido, foi desenvolvido um projeto de extensão fim disseminar informações baseadas literatura científica atual sobre aspectos nutricionais que influenciam qualidade vida infantil. Esse estudo tem como objetivo descrever os resultados obtidos após aplicação do “Nutrição e afeto: introdução alimentar”. Este por acadêmicos curso medicina, com...
Additional apple juice extraction with pulsed electric field pre-treated cubes towards control samples is evaluated. Monopolar and bipolar shaped pulses are compared their effect studied variation of field, pulse width number pulses. Variation strength ranged from 100 V/cm to 1300 V/cm, 20 μs 300 10 200, at frequency 200Hz. Two trains separated by 1 second applied all samples. Bipolar showed higher yields parameters. Calculation specific energies consumed was assessed a threshold where...
This paper describes a new resonant converter topology, using solid-state asymmetric bridge to increase the energy efficiency of switching circuit being designed replace actual pulse transformer and thyratron based modulator that supplies 60 kV target potential for ion acceleration On-Line Isotope Mass Separator ISOLDE accelerator. The preliminary results 5 laboratory prototype validate this concept, however design all components, including high-voltage coil, as well as, selection...
In this paper a voltage droop compensation based on resonant circuit is proposed for the optimized solid-state bipolar Marx modulator. Keeping modularity characteristics and topology, one auxiliary stage was added to existing stages. The concept consists of adding output positive or negative pulse compensation. Simulation results are presented five stages circuit, 10% droop, using 800 V per stage, 100 ps duration at 50Hz frequency.
Side-chain modeling has a widespread application in many current methods for protein tertiary structure determination, prediction, and design. Of the existing side-chain methods, rotamer-based are fastest most efficient. Classically, rotamer is conceived as single, rigid conformation of an amino acid side-chain. Here, we present flexible model which continuous ensemble conformations that cluster around classic rotamer. We have developed thermodynamically based method calculating effective...