A5086362490- Genetically Modified Organisms Research
- Transgenic Plants and Applications
- Food Allergy and Anaphylaxis Research
- Salmonella and Campylobacter epidemiology
- Education Pedagogy and Practices
- Nutrition, Genetics, and Disease
- Listeria monocytogenes in Food Safety
- Consumer Attitudes and Food Labeling
- CRISPR and Genetic Engineering
- Statistics Education and Methodologies
- Identification and Quantification in Food
- Plant tissue culture and regeneration
- Insect Resistance and Genetics
- Education and Digital Technologies
- Monoclonal and Polyclonal Antibodies Research
- Animal Genetics and Reproduction
- Chemistry Education and Research
- Vibrio bacteria research studies
- International Relations and Autism
- Aquaculture disease management and microbiota
- Allergic Rhinitis and Sensitization
- Bioeconomy and Sustainability Development
- Biotechnology and Related Fields
- Antibiotic Resistance in Bacteria
- Photosynthetic Processes and Mechanisms
National Institute of Health Dr. Ricardo Jorge
2012-2024
Universidade Federal de Pernambuco
2015-2022
Universidade Nova de Lisboa
1995-2014
Instituto de Biologia Experimental e Tecnológica
2005-2009
Instituto Nacional de Saúde
2005-2007
Instituto de Tecnología Química
2005
Controversy regarding genetically modified (GM) plants and their potential impact on human health contrasts with the tacit acceptance of other that were also modified, but not considered as GM products (e.g., varieties raised through conventional breeding such mutagenesis). What is beyond phenotype these improved plants? Should mutagenized be treated differently from transgenics? We have evaluated extent transcriptome modification occurring during rice improvement transgenesis versus...
Listeria monocytogenes, a foodborne pathogen, exhibits high adaptability to adverse environmental conditions and is common in the food industry, especially ready-to-eat foods. L. monocytogenes strains pose safety challenges due their ability form biofilms, increased resistance disinfectants, long-term persistence environment. The aim of this study was evaluate presence genetic diversity related products collected from 2014 2022 assess antibiotic susceptibility biofilm formation abilities....
Traditional cheeses are part of the Portuguese gastronomic identity, and raw milk autochthonous species is a common primary ingredient. Here, we investigated presence Listeria monocytogenes, Coagulase Positive Staphylococci (CPS) pathogenic Escherichia coli, as well indicator microorganisms (E. coli other spp.) in 96 cured from Alentejo region. Whole genome sequencing (WGS) E. spp. antimicrobial resistance (AMR) screening isolates was also performed. L. CPS > 104 cfu/g Extraintestinal...
Objetivo: Identificar o nível de conhecimento adolescentes gestantes sobre métodos contraceptivos e sua utilização em uma cidade do interior Goiás. Método: Trata-se um estudo qualitativo, transversal descritivo, realizado entre setembro novembro 2021, três Unidades Básicas Saúde. Participaram 10 gestantes, com idades 19 anos. A análise incluiu caracterização sociodemográfica aplicação da Teoria Fundamentada nos Dados (TFD). Resultados: As entrevistas identificaram categorias como: primeira...
Objetivo: Discutir a atuação de profissionais da saúde no acompanhamento crianças com Transtorno do Espectro Autista. Metodologia: pesquisa descritivo-exploratória abordagem qualitativa. Participaram 5 que realizam terapia/tratamento pessoas TEA na Associação Pais e Amigos dos Excepcionais (APAE) Centro Reabilitação em Terapia Ocupacional (CERETO). A coleta dados ocorreu período setembro 2018 março 2019, por meio realização Entrevistas. Quanto as Entrevista informações colhidas foram...
Abstract More than 15% of Brazil’s urban population lives in slums characterized by limited access to essential services, heightened vulnerability infectious pathogens and environmental hazards, deprivation citizenship rights. These conditions exacerbate social inequality, perpetuate cycles poverty, fuel violence, underscoring the urgent need for sustainable interventions. Following a justice framework, we developed community development program rooted participatory research methods popular...
No contexto da pandemia do coronavírus-19, a comunidade foi inundada por uma quantidade significativa de fake news, que divulgam, maneira manipulada e distorcida, informações já conhecidas validadas cientificamente. Considerando o fenômeno disruptivo infodemia Covid-19 na Cidade Estrutural DF, pesquisa objetivou analisar as políticas relacionadas à comunicação, difusão promoção acesso conhecimentos técnicos-científicos sobre nesta cidade. Respaldada nas relações bio-poder, propostas...
In spite of being among the main foods responsible for allergic reactions worldwide, soybean (Glycine max)-derived products continue to be increasingly widespread in a variety food due their well-documented health benefits. Soybean also continues one elected target crops genetic modification. The aim this study was characterize soya proteome and, specifically, IgE-reactive proteins as well compare IgE response soya-allergic individuals genetically modified Roundup Ready versus its...
Due to specific bacterial microbiota, raw milk cheeses have appreciated sensory properties. However, they may pose a threat consumer safety due potential pathogens presence. This study evaluated the microbiological contamination of 98 from Beira Baixa, Portugal. Presence and enumeration Coagulase Positive Staphylococci (CPS), Listeria monocytogenes, Salmonella spp., pathogenic Escherichia coli, indicator microorganisms (non-pathogenic E. coli spp.) was attained. antimicrobial resistance...
A seven‐iron ferredoxin was isolated from aerobically grown cells of the hyperthermoacidophilic archaeon Desulfurolobus ambivalens (DSM 3772). The protein is monomeric, with an apparent molecular mass 15 kDa and contains 7 iron atoms/molecule. N‐terminal sequence shows a large similarity (70% identity) that Sulfolobus acidocaldarius. EPR characteristics in both native (oxidized) dithionite‐reduced states this allowed unequivocal identification [3Fe‐4S] 1+/0 center, reduction potential −270 ±...
The approval of genetically modified (GM) crops is preceded by years intensive research to demonstrate safety humans and environment. We recently showed that in vitro culture stress the major factor influencing proteomic differences GM vs. non-GM plants. This made us question number generations needed erase such "memory". also wondered about relevance alterations promoted transgenesis as compared environment-induced ones. Here we followed three rice lines (1-control, 1-transgenic 1-negative...
Salmonella enterica serovars Heidelberg and Minnesota frequently display several genetic mobile elements making them potential spreaders of resistance genes. Here, we phenotypically determined the antibiotic profile subsequently performed whole-genome sequencing on 36 isolates recovered from samples fresh poultry meat, within Portuguese Official Inspection Plan for Imported Foodstuffs. Several both showed high relatedness either with raw meat imported to Netherlands Brazil or broiler...
The effects of the ectopic expression a barley transcription factor (HvCBF4) under control constitutive (maize Ubi1) or stress-inducible (Arabidopsis RD29A) promoter in abiotic stress response rice (Oryza sativa L.) was investigated. transformed plants were analyzed both at molecular and physiological level AtRD29A::HvCBF4 further using GeneChip® genome array conditions. Only constitutively expressing HvCBF4 have shown increased survival to drought stress, but not cold high-salinity. These...
Allergies are nearly always triggered by protein molecules and the majority of individuals with documented immunologic reactions to foods exhibit IgE hypersensitivity reactions. In this study we aimed understand if natural differences, at proteomic level, between maize populations, may induce different binding proteins profiles among maize-allergic individuals. We also intended deepen our knowledge on proteins.In order accomplish goal have used tools (SDS-PAGE 2-D gel electrophoresis...
Identification of differences between genetically modified plants and their original counterparts plays a central role in risk assessment strategy. Our main goal was to better understand the relevance transgene presence, genetic, epigenetic changes induced by insertion, vitro culture putative unintended transgenic its comparator. Thus, we have used multiplex fluorescence 2DE coupled with MS characterize proteome three different rice lines ( Oryza sativa L. ssp. japonica cv. Nipponbare):...
Human-wildlife coexistence may increase the potential risk of direct transmission emergent or re-emergent zoonotic pathogens to humans. Intending assess occurrence three important foodborne in wild animals two wildlife conservation centers Portugal, we investigated 132 fecal samples for presence
This study aims to analyse the reasoning that children and adults with same school level use assess justify fairness of games, considering aspects probability such as randomness, sample space, comparison probabilities. Data collection included a Piagetian clinical interview based on games chance. The results showed participants’ judgments about depends mainly understanding independence events, analysis perception proportionality when comparing probabilities, they have misunderstandings these...
Birds are potential carriers of pathogens affecting humans and agriculture. Aiming to evaluate the occurrence top three most important foodborne in free-living birds Portugal, we investigated 108 individual fecal samples from one pooled sample gull feces (n = 50) for presence Escherichia coli (pathogenic non-pathogenic), Salmonella spp. Campylobacter Virulence- antimicrobial resistance- (AMR) associated genes were detected by PCR Whole-Genome Sequencing (WGS), phenotypic (serotyping AMR...
Goji berries' bioactive compounds, which allowed classifying them as superfruits, led to an enormous increase of its consumption in western countries. However, the potential risk allergy is a concern. In this study, we aimed characterise proteome goji berries (Lycium barbarum) and identify proteins with putative role allergic reaction (IgE-binding proteins). We firstly used two-dimensional (2D) gel electrophoresis followed by mass spectrometry (MS) proteome, then Immunoblot reactivity plasma...