A5010038895- Vitamin K Research Studies
- Genetics, Aging, and Longevity in Model Organisms
- Transgenic Plants and Applications
- Photosynthetic Processes and Mechanisms
- Food Allergy and Anaphylaxis Research
- DNA Repair Mechanisms
- Telomeres, Telomerase, and Senescence
- Identification and Quantification in Food
- Meat and Animal Product Quality
- Plant tissue culture and regeneration
- Monoclonal and Polyclonal Antibodies Research
- Allergic Rhinitis and Sensitization
- Thermal and Kinetic Analysis
- Contact Dermatitis and Allergies
- Plant Virus Research Studies
- Biodiesel Production and Applications
- Vitamin D Research Studies
- Vitamin C and Antioxidants Research
- Photoreceptor and optogenetics research
- Muscle metabolism and nutrition
- Mitochondrial Function and Pathology
- Advanced Proteomics Techniques and Applications
- CRISPR and Genetic Engineering
- Alkaline Phosphatase Research Studies
- Mass Spectrometry Techniques and Applications
Universidade Nova de Lisboa
2016-2021
University of Aveiro
2014
Rede de Química e Tecnologia
2014
Centro Hospitalar de Lisboa Ocidental
2014
University of Algarve
2013-2014
Algarve Biomedical Center
2014
Maastricht University
2014
Maastricht University Medical Centre
2014
Hospital de Santa Cruz
2014
Vascular and valvular calcifications are pathological processes regulated by resident cells, depending on a complex interplay between calcification promoters inhibitors, resembling skeletal metabolism. Here, we study the role of vitamin K-dependent Gla-rich protein (GRP) in vascular processes.Immunohistochemistry quantitative polymerase chain reaction showed that GRP expression accumulation upregulated with simultaneously osteocalcin matrix Gla (MGP). Using conformation-specific antibodies,...
Gla-rich protein (GRP) was described in sturgeon as a new vitamin-K-dependent (VKDP) with high density of Gla residues and associated ectopic calcifications humans. Although VKDPs function has been related γ -carboxylation, the status GRP humans is still unknown. Here, we investigated expression recently identified spliced transcripts, -carboxylation status, its association calcifications, skin basal cell breast carcinomas. GRP-F1 predominant splice variant expressed healthy cancer tissues....
Evolution of the C
Often plant tissues are recalcitrant and, due to that, methods relying on protein precipitation, such as TCA/acetone precipitation and phenol extraction, usually the of choice for extraction in proteomic studies. However, addition steps may negatively impact recovery, problems associated with re-solubilization. Moreover, we show that when working non-recalcitrant tissues, young maize leaves, compromise maintenance some labile post-translational modifications (PTMs), phosphorylation....
Article22 February 2019Open Access Transparent process Ssu72 phosphatase is a conserved telomere replication terminator Jose Miguel Escandell Corresponding Author [email protected] orcid.org/0000-0003-4857-9413 Instituto Gulbenkian de Ciência, Oeiras, Portugal Search for more papers by this author Edison SM Carvalho Maria Gallo-Fernandez Clara C Reis Samah Matmati Equipe Labellisée Ligue, CRCM, CNRS, Inserm, Institut Paoli-Calmettes, Aix-Marseille University, Marseille, France Inês Matias...
Goji berries' bioactive compounds, which allowed classifying them as superfruits, led to an enormous increase of its consumption in western countries. However, the potential risk allergy is a concern. In this study, we aimed characterise proteome goji berries (Lycium barbarum) and identify proteins with putative role allergic reaction (IgE-binding proteins). We firstly used two-dimensional (2D) gel electrophoresis followed by mass spectrometry (MS) proteome, then Immunoblot reactivity plasma...
Abstract Telomeres, the protective ends of eukaryotic chromosomes, are replicated through concerted actions by conventional DNA polymerases and telomerase, though regulation this process is not fully understood. Telomere replication requires (C)-Stn1-Ten1, a telomere ssDNA-binding complex that homologous to RPA. Here, we show evolutionarily conserved phosphatase Ssu72 responsible for terminating cycle in fission yeast. controls recruitment Stn1 telomeres regulating phosphorylation at S74,...