A5074890335- Metalloenzymes and iron-sulfur proteins
- Metal-Catalyzed Oxygenation Mechanisms
- Hemoglobin structure and function
- Porphyrin Metabolism and Disorders
- Vitamin K Research Studies
- Electrocatalysts for Energy Conversion
- Parathyroid Disorders and Treatments
- Heme Oxygenase-1 and Carbon Monoxide
- Electrochemical Analysis and Applications
- Enzyme function and inhibition
- Enzyme Structure and Function
- Molecular spectroscopy and chirality
- Magnetism in coordination complexes
- Alkaline Phosphatase Research Studies
- Electrochemical sensors and biosensors
- Electron Spin Resonance Studies
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Metal complexes synthesis and properties
- Cancer, Hypoxia, and Metabolism
- Medical Imaging and Pathology Studies
- Protein Structure and Dynamics
- Porphyrin and Phthalocyanine Chemistry
- Redox biology and oxidative stress
- Fermentation and Sensory Analysis
Universidade Nova de Lisboa
2013-2024
Rede de Química e Tecnologia
2006-2021
Unidade em Ciências Biomoleculares Aplicadas
2021
Algarve Biomedical Center
2014-2018
Maastricht University
2014-2018
University of Algarve
2014-2018
VIB-UGent Center for Medical Biotechnology
2018
Hospital de Santa Cruz
2014
University of Aveiro
2014
Maastricht University Medical Centre
2014
Inhibition of mineral crystal formation is a crucial step in ectopic calcification. Serum calciprotein particles (CPPs) have been linked to chronic kidney disease (CKD) calcification propensity, but additional knowledge required understand their function, assemblage, and composition. The role other circulating nanostructures, such as extracellular vesicles (EVs) vascular currently unknown. Here, we investigated the association GRP (Gla-rich protein) with CPP EVs CKD CPPs...
Vascular and valvular calcifications are pathological processes regulated by resident cells, depending on a complex interplay between calcification promoters inhibitors, resembling skeletal metabolism. Here, we study the role of vitamin K-dependent Gla-rich protein (GRP) in vascular processes.Immunohistochemistry quantitative polymerase chain reaction showed that GRP expression accumulation upregulated with simultaneously osteocalcin matrix Gla (MGP). Using conformation-specific antibodies,...
Calcification-related chronic inflammatory diseases are multifactorial pathological processes, involving a complex interplay between inflammation and calcification events in positive feed-back loop driving disease progression. Gla-rich protein (GRP) is vitamin K dependent (VKDP) shown to function as inhibitor cardiovascular articular tissues, proposed an anti-inflammatory agent chondrocytes synoviocytes, acting new crosstalk factor these two interconnected osteoarthritis. However, possible...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTemperature-dependent proton NMR investigation of the electronic structure trinuclear iron cluster oxidized Desulfovibrio gigas ferredoxin IIAnjos L. Macedo, Isabel Moura, Jose J. G. Jean Le Gall, and Huynh Boi HanhCite this: Inorg. Chem. 1993, 32, 7, 1101–1105Publication Date (Print):March 1, 1993Publication History Published online1 May 2002Published inissue 1 March...
Iron−sulfur clusters with [3Fe−4S] cores are widely distributed in biological systems. In the oxidized state, designated [3Fe−4S]+, these electron-transfer agents have an electronic ground state S = 1/2, and they exhibit EPR signals centered at g 2.01. It has been established by Mössbauer spectroscopy that three iron sites of cluster high-spin Fe3+, general properties 1/2 described exchange Hamiltonian Hexch J12S1·S2 + J23S2·S3 J13S1·S3. Some [3Fe−4S]+ (type 1) their g-values confined to...
The recent derivation, based on pure quantum chemistry (QC) first-principles, of the pseudocontact shifts (PCSs) caused by a paramagnetic metal center far away nuclei has cast doubts validity semiempirical (SE) theory, predicting PCSs to arise from magnetic susceptibility anisotropy. SE theory been used and applied countless times, especially in last 2 decades, obtain structural information proteins containing ions. We show here that QC predictions can be directly tested against experiments,...
The direct conversion of plant cell wall polysaccharides into soluble sugars is one the most important reactions on earth, and performed by certain microorganisms such as Clostridium thermocellum ( Ct ). These organisms produce extracellular multi‐subunit complexes (i.e. cellulosomes) comprising a consortium enzymes, which contain noncatalytic carbohydrate‐binding modules (CBM) that increase activity catalytic module. In present study, we describe combined approach X‐ray crystallography, NMR...
The carbon and fluorine chemical shifts of mixtures dioxide Krytox, a carboxylic acid end-capped perfluorinated polyether used as stabilizer for the dispersion polymerization methyl methacrylate, have been studied using high-pressure, high-resolution nuclear magnetic resonance. 13C 19F spectra were measured in density region between 0.54 0.73 g.cm(-3) at 334 K different solutions Krytox scCO2 (0.22, 1.13 1.72 w/w %). An in-house developed high-pressure apparatus with capability to change...
Non-catalytic cellulosomal CBMs (carbohydrate-binding modules) are responsible for increasing the catalytic efficiency of cellulosic enzymes by selectively putting substrate (a wide range poly- and oligo-saccharides) enzyme into close contact. In present study we carried out an atomistic rationalization molecular determinants ligand specificity a family 11 CBM from thermophilic Clostridium thermocellum [CtCBM11 (C. CBM11)], based on NMR modelling approach. We have determined solution...
Desulfovibrio gigas ferredoxin II (FdII) is a small protein (alpha 4 subunit structure as isolated; M(r) approximately 6400 per subunit; 6 cysteine residues) containing one Fe3S4 cluster alpha-subunit. The x-ray of FdII has revealed disulfide bridge formed by Cys-18 and Cys-42 13 A away from the center cluster; moreover, indicates that Cys-11 forms with methanethiol. In oxidized state, FdIIoxm 1H NMR spectra, exhibit four low-field contact-shifted resonances at 29, 24, 18, 15.5 ppm whereas...
Abstract 1D and 2D 1 H NMR studies are reported on the oxidized reduced [4Fe‐4S] cluster of Desulfovibrio gigas ferredoxin I (Fdl). Several low‐field contact shifted resonances (fast relaxing) assigned to β‐CH 2 α‐CH coordinated cysteinyl residues. NOESY patterns (supported by NOE experiments) resolves four pairs geminal protons at low‐field. The ligands non‐specifically Cys8, Cys11, Cys14 Cys50, based X‐ray structural analysis available for oligomeric form, FdII, that contains a single...
The mitochondrial import of 5‐aminolevulinate synthase (ALAS), the first enzyme mammalian heme biosynthetic pathway, requires N‐terminal presequence. 49 amino acid presequence transit peptide (psALAS) for murine erythroid ALAS was chemically synthesized, and circular dichroism 1 H nuclear magnetic resonance (NMR) spectroscopies used to determine structural elements in trifluoroethanol/H 2 O solutions micellar environments. A well defined amphipathic α‐helix, spanning L22 F33, present psALAS...
Understanding the fine structural details of inhibitor binding at active site metalloenzymes can have a profound impact on rational drug design targeted to this broad class biomolecules. Structural techniques such as NMR, cryo-EM, and X-ray crystallography provide bond lengths angles, but uncertainties in these measurements be large range values that been observed for quantities all published structures. This uncertainty is far too allow reliable calculations quantum chemical (QC) levels...
Hirsutellin (HtA) is intermediate in size between other ribotoxins and less specific microbial RNases, thus offers a unique chance to determine the minimal structural requirements for activities ribotoxins. Here, we have determined structure of HtA by NMR methods. The consists one alpha-helix, helical turn seven beta-strands that form an N-terminal hairpin anti-parallel beta-sheet, with characteristic alpha + beta fold highly positive charged surface. Compared its larger homolog...