A5076746491- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Advanced Fluorescence Microscopy Techniques
- Immune Cell Function and Interaction
- Optical Coherence Tomography Applications
- Photoacoustic and Ultrasonic Imaging
- Glycosylation and Glycoproteins Research
- Safe Handling of Antineoplastic Drugs
- Photodynamic Therapy Research Studies
- Optical Imaging and Spectroscopy Techniques
- Advanced Optical Sensing Technologies
- Signaling Pathways in Disease
- Cellular transport and secretion
- IL-33, ST2, and ILC Pathways
- Advanced Biosensing Techniques and Applications
- Ubiquitin and proteasome pathways
Immatics Biotechnologies (Germany)
2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2015-2021
Medigene (Germany)
2021
These guidelines are a consensus work of considerable number members the immunology and flow cytometry community. They provide theory key practical aspects enabling immunologists to avoid common errors that often undermine immunological data. Notably, there comprehensive sections all major immune cell types with helpful Tables detailing phenotypes in murine human cells. The latest techniques applications also described, featuring examples data can be generated and, importantly, how analysed....
Germinal centers (GCs) are the sites where B cells undergo affinity maturation. The regulation of cellular output from GC is not well understood. Here, we show that earliest stages response, plasmablasts emerge at GC–T zone interface (GTI). We define two main factors regulate this process: Tfh-derived IL-21, which supports production GC, and TNFSF13 (APRIL), produced by a population podoplanin+ CD157high fibroblastic reticular located in GTI also rich message for IL-6 chemokines CXCL12,...
Rationale of the trial Although use engineered T cells in cancer immunotherapy has greatly advanced treatment hematological malignancies, reaching meaningful clinical responses solid tumors is still challenging. We investigated safety and tolerability IMA202 a first-in-human, dose escalation basket human leucocyte antigen A*02:01 positive patients with melanoma-associated A1 (MAGEA1)-positive tumors. Trial design The 2+2 was an algorithmic based on maximally acceptable dose-limiting toxicity...
Abstract Simultaneous detection of multiple cellular and molecular players in their native environment, one the keys to a full understanding immune processes, remains challenging for vivo microscopy. Here, we present synergistic strategy spectrally multiplexed imaging composed (i) triple two-photon excitation using spatiotemporal synchronization two femtosecond lasers, (ii) broad set fluorophores with emission ranging from blue near infrared, (iii) an effective spectral unmixing algorithm....
Abstract Siglec-G, a member of the sialic acid–binding Ig-like lectin (Siglec) family, is expressed on B cell and dendritic surfaces. It acts as an inhibitory coreceptor modulates activation, especially B1 cells, Siglec-G–deficient mice show mainly cell–restricted phenotype resulting in increased numbers. Although higher numbers are discussed to be associated with autoimmunity, loss Siglec-G does not result autoimmune disease BALB/c mice. However, there evidence from × CD22 double-deficient...
Autoantibodies against double-stranded DNA (anti-dsDNA) are a hallmark of systemic lupus erythematosus (SLE). It is well-documented that anti-dsDNA reactive B lymphocytes normally controlled by immune self-tolerance mechanisms operating at several levels. The evolution high levels IgG in SLE dependent on somatic hypermutation and clonal selection, presumably germinal centers from non-autoreactive cells. Twin studies as well genetic mice indicate very strong contribution for the development...
Abstract Germinal center reactions are established during a thymus-dependent immune response. (GC) B cells rapidly proliferating and undergo somatic hypermutation in Ab genes. This results the production of high-affinity Abs establishment long-lived memory cells. GC show lower BCR-induced signaling when compared with naive cells, but functional relevance is not clear. CD22 member Siglec family functions as an inhibitory coreceptor on Interestingly, downregulate sialic acid forms that serve...
Two-photon microscopy enables monitoring cellular dynamics and communication in complex systems, within a genuine environment, such as living tissues and, even, organisms. Particularly, its application to understand interactions the immune system has brought unique insights into pathophysiologic processes vivo. Simultaneous multiplexed imaging is required dynamic orchestration of multiple non-cellular tissue compartments defining responses. Here, we present an improvement our previously...
The proper differentiation and maintenance of myofibers is fundamental to a functional musculature. Disruption numerous mostly structural factors leads perturbations these processes. Among the limited number known regulatory for processes Mind bomb2 (Mib2), muscle-associated E3 ubiquitin ligase, which was previously established be required maintaining integrity larval muscles. In this study, we have examined mechanistic aspects Mib2 function by performing detailed dissection protein. We show...
<h3>Background</h3> Transgenic T cell receptor (TCR)-based therapies are a powerful treatment for cancer. However, one of the greatest remaining challenges is successful identification tumor-specific antigens (TSAs) that shared between patients and tumor entities elicit strong responses. The non-coding region genome has become promising source such novel TSAs. Previously, we have identified ten immunogenic TSAs, derived from translation in canonical non-canonical reading frames non-mutated...