A5074144759- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Gene expression and cancer classification
- Transgenic Plants and Applications
- Plant Stress Responses and Tolerance
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Plant Genetic and Mutation Studies
- Single-cell and spatial transcriptomics
- Microfluidic and Bio-sensing Technologies
- Horticultural and Viticultural Research
- Plant Gene Expression Analysis
- Molecular Biology Techniques and Applications
- Genetic Mapping and Diversity in Plants and Animals
- Soybean genetics and cultivation
- Plant Pathogens and Resistance
- Plant Taxonomy and Phylogenetics
- CRISPR and Genetic Engineering
- Monoclonal and Polyclonal Antibodies Research
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
University of Arizona
2015-2024
Janssen (United States)
2024
Henan University
2018-2023
State Key Laboratory of Cotton Biology
2018-2023
Rancho BioSciences (United States)
2021-2023
Bristol-Myers Squibb (United States)
2019-2021
Pennsylvania State University
2021
Planta
1997-2016
University of Arizona Cancer Center
2016
University of California, Riverside
2004-2010
Mechanical chopping of plant tissues in the presence mithramycin released intact nuclei representative cells within tissues. The amount nuclear DNA homogenates monocotyledonous and dicotyledonous plants was accurately rapidly determined by flow microfluorometry, distribution involved cell cycle charted for selected from different physical locations or developmental stages.
A global map of gene expression within an organ can identify genes with coordinated in localized domains, thereby relating activity to cell fate and tissue specialization. Here, we present localization more than 22,000 the Arabidopsis root. Gene was mapped 15 different zones root that correspond types tissues at progressive developmental stages. Patterns traverse traditional anatomical boundaries show cassettes hormonal response. Chromosomal clustering defined some coregulated genes. This...
Transcript regulation in response to high salinity was investigated for salt-tolerant rice (var Pokkali) with microarrays including 1728 cDNAs from libraries of salt-stressed roots. NaCl at 150 mM reduced photosynthesis one tenth the prestress value within minutes. Hybridizations RNA microarray slides probed changes transcripts 15 min 1 week after salt shock. Beginning shock, Pokkali showed upregulation transcripts. Approximately 10% were significantly upregulated or downregulated hr stress....
One of the most important functions plant hormone abscisic acid (ABA) is to induce stomatal closure by reducing turgor guard cells under water deficit. Under environmental stresses, hydrogen peroxide (H(2)O(2)), an active oxygen species, widely generated in many biological systems. Here, using epidermal strip bioassay and laser-scanning confocal microscopy, we provide evidence that H(2)O(2) may function as intermediate ABA signaling Vicia faba cells. inhibited induced stomata, this effect...
Multicellular organs are composed of distinct cell types with unique assemblages translated mRNAs. Here, ribosome-associated mRNAs were immunopurified from specific populations intact seedlings using Arabidopsis thaliana lines expressing a FLAG-epitope tagged ribosomal protein L18 (FLAG-RPL18) via developmentally regulated promoters. The profiling in ribosome complexes, referred to as the translatome, identified differentially expressed 21 defined by cell-specific expression FLAG-RPL18....
The marriage between immunology and cytometry is one of the most stable productive in recent history science. A rapid search PubMed shows that, as July 2017, using "flow immunology" a term yields more than 68 000 articles, first which, interestingly, not about lymphocytes. It might be stated after short engagement, exchange wedding rings officially occurred when idea to link fluorochromes monoclonal antibodies came about. After this, recognizing different types cells became relatively easy...
Abstract Salt cress (Thellungiella halophila) is a small winter annual crucifer with short life cycle. It has genome (about 2 × Arabidopsis) high sequence identity (average 92%) Arabidopsis, and can be genetically transformed by the simple floral dip procedure. capable of copious seed production. an extremophile native to harsh environments reproduce after exposure extreme salinity (500 mm NaCl) or cold −15°C. typical halophyte that accumulates NaCl at controlled rates also dramatic levels...
Microfluorometric analysis of the nuclear DNA contents somatic tissues Arabidopsis thaliana has revealed extensive endoreduplication, resulting in that comprise mixtures polyploid cells. Endoreduplication was found all except those inflorescences and developmentally regulated according to age their position within plant.
Flax (Linum usitatissimum) is an ancient crop that widely cultivated as a source of fiber, oil and medicinally relevant compounds. To accelerate improvement, we performed whole-genome shotgun sequencing the nuclear genome flax. Seven paired-end libraries ranging in size from 300 bp to 10 kb were sequenced using Illumina analyzer. A de novo assembly, comprised exclusively deep-coverage (approximately 94× raw, approximately 69× filtered) short-sequence reads (44-100 bp), produced set scaffolds...
Significance One of the central goals developmental biology and medicine is to ascertain relationships between genotype phenotype cells. Single-cell transcriptome analysis represents a powerful strategy reach this goal. We advance these strategies single nuclei from neural progenitor cells dentate gyrus tissue, which it very difficult recover intact This provides unique means carry out RNA sequencing individual neurons that avoids requiring isolation single-cell suspensions, eliminating...
The persistent cereal endosperm constitutes the majority of grain volume. Dissecting gene regulatory network underlying development will facilitate yield and quality improvement crops. Here, we use single-cell transcriptomics to analyze developing maize (Zea mays) during cell differentiation. After obtaining transcriptomic data from 17,022 single cells, identify 12 clusters corresponding five types revealing complex transcriptional heterogeneity. We delineate temporal gene-expression pattern...
Auxins are growth regulators involved in virtually all aspects of plant development. However, little is known about how plants synthesize these essential compounds. We propose that the level indole-3-acetic acid regulated by flux indole-3-acetaldoxime through a cytochrome P450, CYP83B1, to glucosinolate pathway. A T-DNA insertion CYP83B1 gene leads with phenotype suggests severe auxin overproduction, whereas overexpression loss apical dominance typical deficit. N-hydroxylates corresponding...
The green‐fluorescent protein (GFP) from jellyfish Aequorea victoria has been used as a convenient new vital marker in various heterologous systems. However, it problematic to express GFP higher eukaryotes, especially plants. This paper reports that either strong constitutive or heat‐shock promoter can direct the expression of which is easily detectable maize mesophyll protoplasts. In this single‐cell system, bright green fluorescence emitted visible when excited with UV blue light even...
Flow cytometry was used to compare 14 potential reference standards for plant DNA content determination. Both chicken and internal were used, as propidium iodide (PI) 4'‐6‐diamidino‐2‐phenylindole (DAPI) fluorochromes. Means standard errors of the means are presented standards, compared those obtained by Feulgen densitometry. Five species recommended an initial set international future determinations: Sorghum bicolor cv. Pioneer 8695 (2C = 1.74 pg), Pisum sativum Minerva Maple 9.56 Hordeum...
Immunoaffinity purification of polyribosomes (polysomes) from crude leaf extracts Arabidopsis (Arabidopsis thaliana) was achieved with transgenic genotypes that overexpress a translational fusion ribosomal protein (RP) His(6)-FLAG dual epitope tag. In plants cauliflower mosaic virus 35S:HF-RPL18 transgene immunopurification anti-FLAG agarose beads yielded 60-Svedberg subunits, intact 80-Svedberg monosomes and polysomes. Sucrose density gradient fractionation the purified complexes...
Using flow cytometric analysis of fluorescence, we measured the genome sizes 18 cultured “free‐living” species and 29 Symbiodinium spp. isolates from stony corals, gorgonians, anemones, jellyfish, giant clams. Genome size directly correlated with cell size, as documented previously for most eukaryotic lines. Among smallest dinoflagellates, (6–15 μm) possessed lowest DNA content that (1.5–4.8 pg·cell −1 ). Bloom‐forming or potentially harmful in genera Alexandrium , Karenia Pfiesteria...
Focused and nontargeted approaches were used to assess the impact associated with introduction of new high-flux pathways in Arabidopsis thaliana by genetic engineering. Transgenic A. plants expressing entire biosynthetic pathway for tyrosine-derived cyanogenic glucoside dhurrin as accomplished insertion CYP79A1 , CYP71E1 UGT85B1 from Sorghum bicolor shown accumulate 4% dry-weight marginal inadvertent effects on plant morphology, free amino acid pools, transcriptome, metabolome. In a similar...
Summary The green‐fluorescent protein (GFP) from Aequorea victoria has been shown to be a convenient and flexible reporter molecule within variety of eukaryotic systems, including higher plants. It is particularly suited for applications in vivo , since the mechanism fluorophore formation involves an intramolecular autoxidation does not require exogenous co‐factors. Unlike standard histochemical procedures fixation staining required analysis cellular or tissue‐specific expression other...
Nuclei from Mesembryanthemum crystallinum (ice plant) exhibit multiple levels of ploidy in every tissue as revealed by flow microfluorometric analysis isolated nuclei stained with mithramycin. Multiples the haploid nuclear genome complement (1C) corresponding to 2C, 4C, 8C, 16C, 32C, and 64C were observed. The distribution among different is tissue-specific leaves characteristic stage development. This type organization has been identified eight other succulent CAM (crassulacean acid...
Rice ( Oryza sativa L.) is the most important food crop in world and a model system for plant biology. With completion of finished genome sequence we must now functionally characterize rice by variety methods, including comparative genomic analysis between cereal species within genus Oryza. contains two cultivated 22 wild that represent 10 distinct types. The contain an essentially untapped reservoir agriculturally genes be harnessed if are to maintain safe secure supply 21st century. As...