John D. McPherson
A5047690257- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- Genomic variations and chromosomal abnormalities
- Pancreatic and Hepatic Oncology Research
- Genomics and Rare Diseases
- Genetic factors in colorectal cancer
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Prostate Cancer Treatment and Research
- Acute Myeloid Leukemia Research
- Lung Cancer Treatments and Mutations
- Gene expression and cancer classification
- Acute Lymphoblastic Leukemia research
- Neurogenetic and Muscular Disorders Research
- RNA regulation and disease
- Cell Image Analysis Techniques
- Colorectal Cancer Treatments and Studies
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
University of California, Davis
2016-2025
Ontario Institute for Cancer Research
2014-2024
Washington University in St. Louis
1997-2023
UC Davis Comprehensive Cancer Center
2004-2022
University of California System
1993-2022
University of Toronto
2010-2019
UC Davis Health System
2019
University Health Network
2012-2018
University of California Davis Medical Center
2017
Comprehensive Blood & Cancer Center
2017
The human genome holds an extraordinary trove of information about development, physiology, medicine and evolution. Here we report the results international collaboration to produce make freely available a draft sequence genome. We also present initial analysis data, describing some insights that can be gleaned from sequence.
Somatic alterations in cellular DNA underlie almost all human cancers. The prospect of targeted therapies and the development high-resolution, genome-wide approaches are now spurring systematic efforts to characterize cancer genomes. Here we report a large-scale project copy-number primary lung adenocarcinomas. By analysis large collection tumours (n = 371) using dense single nucleotide polymorphism arrays, identify total 57 significantly recurrent events. We find that 26 39 autosomal...
Intratumoral heterogeneity arises through the evolution of genetically diverse subclones during tumor progression. However, it remains unknown whether cells within single genetic clones are functionally equivalent. By combining DNA copy number alteration (CNA) profiling, sequencing, and lentiviral lineage tracking, we followed repopulation dynamics 150 lentivirus-marked lineages from 10 human colorectal cancers serial xenograft passages in mice. CNA mutational analysis distinguished...
In a classical view of hematopoiesis, the various blood cell lineages arise via hierarchical scheme starting with multipotent stem cells that become increasingly restricted in their differentiation potential through oligopotent and then unipotent progenitors. We developed cell-sorting to resolve myeloid (My), erythroid (Er), megakaryocytic (Mk) fates from single CD34(+) mapped progenitor hierarchy across human development. Fetal liver contained large numbers distinct progenitors intermingled...
The zebrafish is an important vertebrate model for the mutational analysis of genes effecting developmental processes. Understanding relationship between and mutations with those humans will require understanding syntenic correspondence human genomes. High throughput gene EST mapping projects in are now facilitating this goal. Map positions 523 ESTs predicted orthologs reveal extensive contiguous blocks synteny Eighty percent analyzed belong to conserved groups (two or more linked both...
The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing complete open reading frame (ORF) for every human mouse gene. initially used random strategy select clones from large number libraries diverse tissues. Candidate were chosen based on 5′-EST sequences, then fully sequenced high accuracy analyzed by algorithms developed this project. Currently, more than 11,000 10,000 genes are...
As part of the Human Genome Project, Washington University Sequencing Center has commenced systematic sequencing human chromsome 7. To organize and supply effort, we have undertaken construction sequence-ready physical maps for defined chromosomal intervals. Map is a serial process composed three main activities. First, candidate STS-positive large-insert PAC BAC clones are identified. Next, these subjected to fingerprint analysis. Finally, data used assemble maps. The fingerprinting method...
The human apo-E gene has been isolated from a X phage library using as probe the previously reported cDNA clone pE-301.X was mapped and subcloned, completely sequenced.The DNA sequence compared with that of near full length pE-368 revealed three introns.The first intron in region corresponds to 5' untranslated mRNA.The second interrupted codon specifying amino acid -4 signal peptide.The third 61 mature protein.Analysis four Alu sequences.Two were opposite orientations intron, one each...