Lauren C. Chong
A5083818639- Cancer Immunotherapy and Biomarkers
- Lymphoma Diagnosis and Treatment
- Immune cells in cancer
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Chronic Lymphocytic Leukemia Research
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related gene regulation
- Ovarian cancer diagnosis and treatment
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Scientific Computing and Data Management
- Molecular Biology Techniques and Applications
- Toxic Organic Pollutants Impact
- RNA modifications and cancer
- Distributed and Parallel Computing Systems
- Cancer Cells and Metastasis
- HER2/EGFR in Cancer Research
- Viral-associated cancers and disorders
- T-cell and Retrovirus Studies
- NF-κB Signaling Pathways
- Ubiquitin and proteasome pathways
- Effects and risks of endocrine disrupting chemicals
BC Cancer Agency
2014-2024
Spinal Cord Injury BC
2018-2024
Thomas Cole Historic House
2024
AbCellera (Canada)
2024
Yale University
2022-2024
University of British Columbia
2014-2023
Royal Stoke University Hospital
2021
Ontario Institute for Cancer Research
2012-2019
Occupational Cancer Research Centre
2019
RELX Group (Netherlands)
2017
BackgroundClinical prognostic groupings for localised prostate cancers are imprecise, with 30–50% of patients recurring after image-guided radiotherapy or radical prostatectomy. We aimed to test combined genomic and microenvironmental indices in cancer improve risk stratification complement clinical factors.MethodsWe used DNA-based alone combination intra-prostatic hypoxia measurements develop four 126 low-risk intermediate-risk (Toronto cohort) who will receive radiotherapy. validated these...
Abstract Purpose: Relapsed or refractory diffuse large B-cell lymphoma (rrDLBCL) is fatal in 90% of patients, and yet little known about its biology. Experimental Design: Using exome sequencing, we characterized the mutation profiles 38 rrDLBCL biopsies obtained at time progression after immunochemotherapy. To identify genes that may be associated with relapse, compared frequency samples relapse to an unrelated cohort 138 diagnostic DLBCLs separately amplified specific mutations their...
Abstract Hodgkin lymphoma is characterized by an extensively dominant tumor microenvironment (TME) composed of different types noncancerous immune cells with rare malignant cells. Characterization the cellular components and their spatial relationship crucial to understanding cross-talk therapeutic targeting in TME. We performed single-cell RNA sequencing more than 127,000 from 22 tissue specimens 5 reactive lymph nodes, profiling for first time phenotype lymphoma–specific at resolution....
Abstract Diffuse large B-cell lymphoma (DLBCL) is an aggressive cancer originating from mature B-cells. Prognosis strongly associated with molecular subgroup, although the driver mutations that distinguish two main subgroups remain poorly defined. Through integrative analysis of whole genomes, exomes, and transcriptomes, we have uncovered genes non-coding loci are commonly mutated in DLBCL. Our has identified novel cis -regulatory sites, implicates recurrent 3′ UTR NFKBIZ as a mechanism...
Summary: flowDensity facilitates reproducible, high-throughput analysis of flow cytometry data by automating a predefined manual gating approach. The algorithm is based on sequential bivariate approach that generates set cell populations. It chooses the best cut-off for individual markers using characteristics density distribution. Supplementary Data linked to online version manuscript. Availability and implementation: R source code freely available through BioConductor...
Primary mediastinal large B cell lymphoma (PMBCL) is an aggressive non-Hodgkin's lymphoma, predominantly affecting young patients. We analyzed 45 primary PMBCL tumor biopsies and 3 PMBCL-derived lines for the presence of genetic alterations involving major histocompatibility complex (MHC) class II transactivator CIITA found frequent aberrations consisting structural genomic rearrangements, missense, nonsense, frame-shift mutations (53% all lines). also detected intron 1 in 47% cases,...
We introduce BPG, a framework for generating publication-quality, highly-customizable plots in the R statistical environment. This open-source package includes multiple methods of displaying high-dimensional datasets and facilitates generation complex multi-panel figures, making it suitable datasets. A web-based interactive tool allows online figure customization, from which code can be downloaded integration with computational pipelines. BPG provides new approach linking scripted data...
Abstract Purpose: Tumor-infiltrating lymphocytes (TIL) are strongly associated with survival in most cancers; however, the tumor-reactive subset that drives this prognostic effect remains poorly defined. CD39, CD103, and PD-1 have been independently proposed as markers of CD8+ TIL various cancers. We evaluated phenotype, clonality, significance expressing combinations these high-grade serous ovarian cancer (HGSC), a malignancy need more effective immunotherapeutic approaches. Experimental...
Significance Our study provides detailed functional and spatial characteristics of immune cells in the LR-CHL microenvironment at single-cell resolution. We describe T cell subset definitions importantly identified a unique CD4 + PD-1 CXCL13 CXCR5 − TFH-like that surrounds HRS cells, appears close proximity to B is associated with poor clinical outcome. also uncovered PD-1/PD-L1 axis biology LR-CHL, namely negative correlation between PD-L1 genetic alterations on protein expression tumor...
Abstract Multiplexed immune cell profiling of the tumor microenvironment (TME) in cancer has improved our understanding immunology, but complex spatial analyses tumor-immune interactions lymphoma are lacking. Here, we used imaging mass cytometry (IMC) on 33 cases diffuse large B-cell (DLBCL) to characterize and architecture correlate it clinicopathological features such as origin, gene mutations, responsiveness chemotherapy. To understand poor response DLBCL checkpoint inhibitors (ICI),...
Gray zone lymphoma (GZL), a B-cell with features intermediate between large (LBCL) and classic Hodgkin (cHL), is rare poorly defined entity. Alongside GZL, subset of Epstein-Barr virus (EBV)-positive diffuse (DLBCL) has been described polymorphic/GZL-like morphology (polymorphic-EBV-L). To fill the important gap in our understanding pathogenic process underlying these entities, we performed gene expression study international cohort GZL polymorphic-EBV-L, combined cHL primary mediastinal...
About a third of patients with relapsed or refractory classic Hodgkin lymphoma (r/r CHL) succumb to their disease after high-dose chemotherapy followed by autologous stem-cell transplantation (HDC/ASCT). Here, we aimed describe spatially resolved tumor microenvironment (TME) ecosystems establish novel biomarkers associated treatment failure in r/r CHL.
In classical Hodgkin lymphoma (cHL), the highly abundant CD4+ T cells in vicinity of tumor are considered essential for cell survival, but ill-defined. Although they activated, consistently lack expression activation marker CD26. this study, we compared sorted CD4+CD26- and CD4+CD26+ from cHL lymph node suspensions by RNA sequencing receptor variable gene segment usage analysis. This revealed that although antigen experienced, have not clonally expanded. may well be explained exhaustion...
PRAME is a prominent member of the cancer testis antigen family proteins, which triggers autologous T cell-mediated immune responses. Integrative genomic analysis in diffuse large B cell lymphoma (DLBCL) uncovered recurrent and highly focal deletions 22q11.22, including gene, were associated with poor outcome. PRAME-deleted tumors showed cytotoxic escape cold tumor microenvironments. In addition, downmodulation was strongly somatic EZH2 Y641 mutations DLBCL. turn, PRC2-regulated genes...