A5070759686- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Ovarian cancer diagnosis and treatment
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Chromosomal and Genetic Variations
- Congenital Heart Disease Studies
- Radiopharmaceutical Chemistry and Applications
- Adolescent and Pediatric Healthcare
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Renal and related cancers
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- Ferroptosis and cancer prognosis
- Peptidase Inhibition and Analysis
- Williams Syndrome Research
- Multiple Myeloma Research and Treatments
- Congenital heart defects research
- Advanced Proteomics Techniques and Applications
University of British Columbia
2021-2024
Breast Cancer Research Foundation
2023-2024
Bee Research Institute
2023-2024
Institute for Research in Immunology and Cancer
2012-2021
Université de Montréal
2013-2021
A proteogenomic method identifies potentially actionable tumor-specific antigens and shows that most of them are not coded by classic exons.
Abstract In view of recent reports documenting pervasive translation outside canonical protein-coding sequences, we wished to determine the proportion major histocompatibility complex (MHC) class I-associated peptides (MAPs) derived from non-canonical reading frames. Here perform proteogenomic analyses MAPs eluted human B cells using high-throughput mass spectrometry probe six-frame B-cell transcriptome. We report that ∼10% originate allegedly noncoding genomic sequences or exonic...
For decades, the global impact of genomic polymorphisms on repertoire peptides presented by major histocompatibility complex (MHC) has remained a matter speculation. Here we present novel approach that enables high-throughput discovery polymorphic MHC class I-associated (MIPs), which play role in allorecognition. On basis comprehensive analyses landscape MIPs eluted from B lymphoblasts two MHC-identical siblings, show 0.5% non-synonymous single nucleotide variations are represented MIP...
Abstract Purpose: Tumor-infiltrating lymphocytes (TIL) are strongly associated with survival in most cancers; however, the tumor-reactive subset that drives this prognostic effect remains poorly defined. CD39, CD103, and PD-1 have been independently proposed as markers of CD8+ TIL various cancers. We evaluated phenotype, clonality, significance expressing combinations these high-grade serous ovarian cancer (HGSC), a malignancy need more effective immunotherapeutic approaches. Experimental...
High-grade serous ovarian cancer (HGSC), the principal cause of death from gynecologic malignancies in world, has not significantly benefited advances immunotherapy. Although HGSC infiltration by lymphocytes correlates with superior survival, nature antigens that can elicit anti-HGSC immune responses is unknown. The goal this study was to establish global landscape tumor-specific (TSA) using a mass spectrometry pipeline interrogated all reading frames genomic regions. In 23 tumors, we...
High-grade serous ovarian cancer (HGSC) is a challenging disease, especially for patients with immunologically "cold" tumors devoid of tumor-infiltrating lymphocytes (TILs). We found that HGSC exhibits among the highest levels
Transposable elements (TEs) are repetitive sequences representing ~45% of the human and mouse genomes highly expressed by medullary thymic epithelial cells (mTECs). In this study, we investigated role TEs on T-cell development in thymus. We performed multiomic analyses to elucidate their development. report that TE expression thymus is high shows extensive age- cell lineage-related variations. correlates with multiple transcription factors all types Two express particularly broad...
BACKGROUND. Despite an overall poor prognosis, about 15% of patients with advanced-stage tubo-ovarian high-grade serous carcinoma (HGSC) survive ten or more years after standard treatment. METHODS. We evaluated the tumor microenvironment this exceptional, understudied group using a large international cohort enriched for long-term survivors (LTS; 10+ years; n = 374) compared to medium-term (MTS; 5–7.99 433) and short-term (STS; 2–4.99 416). Primary samples were immunostained scored...
In a context where injection of antigen (Ag)-specific T cells probably represents the future leukemia immunotherapy, identification optimal target Ags is crucial. We therefore sought to discover reliable marker for selection most potent Ags. To this end, (1) we immunized mice against 8 individual Ags: 4 minor histocompatibility (miHAs) and leukemia-associated (LAAs) that were overexpressed on leukemic relative normal thymocytes; (2) assessed their ability reject EL4 cells; (3) correlated...
Transposable elements (TE) are repetitive sequences representing ∼45% of the human and mouse genomes highly expressed by medullary thymic epithelial cells (mTEC). In this study, we investigated role TEs on T-cell development in thymus. We performed multi-omic analyses to elucidate their T cell development. report that TE expression thymus is high shows extensive age- lineage-related variations. correlates with multiple transcription factors all types Two express particularly broad...
Transposable elements (TEs) are repetitive sequences representing ~45% of the human and mouse genomes highly expressed by medullary thymic epithelial cells (mTECs). In this study, we investigated role TEs on T-cell development in thymus. We performed multiomic analyses to elucidate their development. report that TE expression thymus is high shows extensive age- cell lineage-related variations. correlates with multiple transcription factors all types Two express particularly broad...
Abstract Transposable elements (TE) are repetitive sequences representing ∼45% of the human and mouse genomes highly expressed by medullary thymic epithelial cells (mTEC). In this study, we investigated role TEs on T-cell development in thymus. We performed multi-omic analyses to elucidate their T cell development. report that TE expression thymus is high shows extensive age- lineage-related variations. correlates with multiple transcription factors all types Two express particularly broad...