A5066738415- HIV Research and Treatment
- HIV/AIDS Research and Interventions
- Immune Cell Function and Interaction
- HIV-related health complications and treatments
- Cytomegalovirus and herpesvirus research
- Hepatitis C virus research
- HIV/AIDS drug development and treatment
- T-cell and B-cell Immunology
- Hepatitis B Virus Studies
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- CAR-T cell therapy research
- Pneumocystis jirovecii pneumonia detection and treatment
- HIV, Drug Use, Sexual Risk
- Immunodeficiency and Autoimmune Disorders
- Reproductive System and Pregnancy
- Hematopoietic Stem Cell Transplantation
- Herpesvirus Infections and Treatments
- Systemic Lupus Erythematosus Research
- Telomeres, Telomerase, and Senescence
- Pneumonia and Respiratory Infections
- COVID-19 Impact on Reproduction
- Monoclonal and Polyclonal Antibodies Research
- Virology and Viral Diseases
Emory University
2024-2025
Université de Montréal
2015-2024
Centre Hospitalier Universitaire Sainte-Justine
2015-2024
Université du Québec à Montréal
2024
GTx (United States)
2023
Occupational Cancer Research Centre
2017
Sorbonne Université
2010
Le Cordon Bleu College of Culinary Arts
2010
Bipar
2010
Montreal Clinical Research Institute
1993-2000
Following infection of the host with a virus, delicate balance between virus replication/spread and immune response to determines outcome infection, i.e., persistence versus elimination virus. It is unclear, however, what relative roles immunologic virologic factors play during primary viral in determining subsequent clinical outcome. By studying cohort subjects HIV it has been demonstrated that qualitative differences HIV, but not quantitative initial levels viremia are associated different...
We describe a novel nucleoside analog, 2'-deoxy-3'-thiacytidine (BCH-189), in which the 3' carbon of ribose ring 2'-deoxycytidine has been replaced by sulfur atom. In MT-4 T cells, this compound had significant time- and dose-dependent antiviral activity against five different strains human immunodeficiency virus type 1 (HIV-1) (mean 50% inhibitory dose, 0.73 microM); known 3'-azido-3'-deoxythymidine (AZT)-resistant HIV-1 variants did not exhibit cross-resistance to it. BCH-189 also...
Therapies based on enhancing the numbers and/or function of T regulatory cells (Tregs) represent one most promising approaches to restoring tolerance in many immune-mediated diseases. Several groups have investigated whether human Tregs suitable for cellular therapy can be obtained by vitro expansion, conversion conventional into Tregs, or gene transfer FOXP3 transcription factor. To date, however, none these has resulted a homogeneous and stable population that is as potently suppressive ex...
Down-regulation of the initial burst viremia during primary HIV infection is thought to be mediated predominantly by HIV-specific cytotoxic T lymphocytes, and appearance this response associated with major perturbations cell receptor repertoire. Changes in repertoire virus-specific lymphocytes were analyzed patients understand failure cellular immune control viral spread replication. This analysis demonstrated that a significant number clones involved rapidly disappeared. The disappearance...
We determined whether drug-resistant variants of HIV-1 could be isolated from the peripheral blood mononuclear cells 20 individuals with HIV infection (Centers for Disease Control groups II and III) on long-term zidovudine (AZT) therapy. Toward this end, (10μM) has been included in tissue culture medium used to isolate HIV-1. Under these circumstances, virus a zidovudine-resistant phenotype was successfully obtained five out cases. This property drug resistance appeared stable, did not...
Th17 cells are permissive to HIV-1 infection and their depletion from the gut of infected individuals leads microbial translocation, a major cause for non-AIDS co-morbidities. Most recent evidence supports contribution long-lived HIV persistence during antiretroviral therapy (ART). However, identity remains unknown. Here, we performed an in-depth transcriptional functional characterization four distinct subsets investigated reservoir ART. In addition previously characterized CCR6+CCR4+...
Background. Individuals infected with human immunodeficiency virus (HIV) appear to age faster than the general population, possibly related HIV infection, antiretroviral therapy, and/or social/environmental factors. We evaluated leukocyte telomere length (LTL), a marker of cellular aging, in HIV-infected and uninfected adults. Methods. Clinical data blood were collected from Children women: AntiRetrovirals Mechanism Aging (CARMA) cohort study participants. Variables found be important...
Infants born prematurely are highly vulnerable to infections and also exhibit a high susceptibility organ damage due inflammation.To investigate homeostatic immune control early in life, we used advanced multiparameter flow cytometry compare responses multiple Toll-like receptor (TLR) ligands single cells mononuclear cell populations term neonates versus preterm before 29 weeks of gestation.Preterm had globally attenuated TLR-stimulated interleukin (IL)-6, interferon-α, and, lesser extent,...
We studied a cohort of children initiated on treatment doses combination antiretroviral therapy within 72 hours birth. In who achieved sustained virologic suppression, measures HIV-1 reservoir size in peripheral blood were very low.
Little is known on outcomes after transition to adult care among adolescents with perinatal HIV infection. Though there data from other chronic pediatric diseases suggesting increased morbidity and mortality following transfer care, this has not well been studied the first wave of survivors The primary objective study was determine a cohort HIV-infected in Québec, Canada. Secondary objectives were document participant experiences process, identify barriers successful transition, potential...
Human immunodeficiency virus (HIV) persistence during antiretroviral therapy (ART) is associated with heightened plasma interleukin-10 (IL-10) levels and PD-1 expression. We hypothesized that IL-10 blockade would lead to control of viral rebound following analytical treatment interruption (ATI). Twenty-eight ART-treated, simian (SIV)mac
<title>Abstract</title> Immunotherapeutic approaches to eliminate latently HIV-infected cells are focused on the adaptive immune system. Herein we provide mechanistic evidence for a molecular cascade characterized by epigenetic reprogramming of innate myeloid and CD4 T cells. The coordinate regulation gene expression mediated transcription factors (TFs) IRF3, IRF7, STAT1 C/EBPβ versus AP-1, promoted development antiviral immunity in these which was associated with control viral load decay...
Nucleoside reverse transcriptase inhibitors (NRTIs) used in HIV antiretroviral therapy can inhibit human telomerase transcriptase. We therefore investigated whether utero or childhood exposure to NRTIs affects leukocyte telomere length (LTL), a marker of cellular aging.In this cross-sectional CARMA cohort study, we factors associated with LTL HIV-1-infected (HIV(+)) children (n = 94), HIV-1-exposed uninfected (HEU) who were exposed (ART) perinatally 177), and HIV-unexposed (HIV(-)) control...
Reports of increased morbidity and mortality from infectious diseases among HIV Exposed Uninfected (HEU) infants have raised concern about a possible underlying immunodeficiency them. The objective this study was to assess the immunological profile HEU born mothers exhibiting different levels HIV-1 viremia at time delivery. Study subjects were enrolled in Centre maternel et infantile sur le SIDA (CMIS) mother-child cohort between 1997 2010 (n =585). Infant CD4+ T cell, CD8+ cell CD19+ B...
We analyzed the T-cell receptor (TCR) V beta repertoire in human immunodeficiency virus type 1 (HIV-1)-infected individuals at different stages of disease. To circumvent effect HLA and other loci on expressed TCR repertoire, we compared nine pairs monozygotic twins who were discordant for HIV infection. A semiquantitative polymerase chain reaction (PCR) assay flow cytometry enabled us to show distinct differences HIV-positive twin with HIV-negative twin. By combining PCR cytofluorometry,...
Abstract Anti-liver-kidney microsome type 1 (LKM1) autoantibodies directed against the cytochrome P450 2D6 (CYP2D6) are considered specific markers of 2 autoimmune hepatitis, but also found in 5% sera from patients chronically infected by hepatitis C virus (HCV). Molecular mimicry between HCV proteins and CYP2D6 has been proposed to explain emergence these autoantibodies. Anti-LKM1 C–infected were affinity-purified immobilized protein used screen a phage display library. conformational...
The dynamics of the memory CD8 T cell receptor (TCR) repertoire upon virus re-exposure and factors governing selection TCR clonotypes conferring protective immunity in real life settings are poorly understood. Here, we examined functionality virus-specific before, during after hepatitis C (HCV) reinfection patients who spontaneously resolved two consecutive infections (SR/SR) a primary but failed to clear subsequent infection (SR/CI). was narrower prior SR/SR group as compared SR/CI became...
We used a viral endpoint dilution assay to show changes in the proportion of zidovudine (azidothymidine; AZT)-resistant viruses within heterogeneous mixture human immunodeficiency virus type 1 (HIV-1) quasispecies isolated from patients on long-term AZT therapy. Several HIV-1 isolates, which could replicate 10 microM AZT, were susceptible both 2',3'-dideoxycytidine and novel cytosine analog BCH-189, sulfur atom replaces 3' carbon pentose ring. In certain instances, cross-resistance was seen...