A5068460951- HIV Research and Treatment
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Toxic Organic Pollutants Impact
- Immunodeficiency and Autoimmune Disorders
- Effects and risks of endocrine disrupting chemicals
- HIV/AIDS drug development and treatment
- Dendrimers and Hyperbranched Polymers
- HIV/AIDS Research and Interventions
- Advanced Polymer Synthesis and Characterization
- Advanced biosensing and bioanalysis techniques
- Cancer Cells and Metastasis
- Angiogenesis and VEGF in Cancer
- Parasitic Infections and Diagnostics
- Drug Transport and Resistance Mechanisms
- Carcinogens and Genotoxicity Assessment
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Fecal contamination and water quality
- Advanced Biosensing Techniques and Applications
- Nanofabrication and Lithography Techniques
- Graphene and Nanomaterials Applications
University of Nottingham
2017-2024
AstraZeneca (United Kingdom)
2024
University of Minho
2022
Park University
2019
Centre Hospitalier de l’Université de Montréal
2009-2017
Université de Montréal
2009-2017
Universidade de São Paulo
2011-2014
Hôpital Saint-Luc
2011
National Institute of Health
2009
Université de Rennes
2007
There is limited knowledge on the identity of primary CD4(+) T cell subsets selectively targeted by HIV-1 in vivo. In this study, we established a link between HIV permissiveness, phenotype/homing potential, and lineage commitment cells. CCR4(+)CCR6(+), CCR4(+)CCR6(-), CXCR3(+)CCR6(+), CXCR3(+)CCR6(-) cells expressed cytokines transcription factors specific for Th17, Th2, Th1Th17, Th1 lineages, respectively. CCR4(+)CCR6(+) CXCR3(+)CCR6(+) coreceptors CCR5 CXCR4 were permissive to R5 X4...
HIV type 1 infection is associated with a rapid depletion of Th17 cells from the GALT. The chemokine receptor CCR6 marker for lineage polarization and permissiveness in memory CD4(+) T cells. CCR6(+) have potential to migrate into GALT via gut-homing integrin α(4)β(7), newly identified HIV-gp120 binding receptor. In this study, we investigated whether coexpressing β(7) are selective targets retinoic acid (RA)-induced imprinting selectively increases cell infection. We demonstrated that...
Th17 cells are permissive to HIV-1 infection and their depletion from the gut of infected individuals leads microbial translocation, a major cause for non-AIDS co-morbidities. Most recent evidence supports contribution long-lived HIV persistence during antiretroviral therapy (ART). However, identity remains unknown. Here, we performed an in-depth transcriptional functional characterization four distinct subsets investigated reservoir ART. In addition previously characterized CCR6+CCR4+...
Gut-associated lymphoid tissues are enriched in CCR6+ Th17-polarized CD4+ T cells that contribute to HIV-1 persistence during antiretroviral therapy (ART). This raises the need for Th17-targeted immunotherapies. In an effort identify mechanisms governing permissiveness/persistence gut-homing Th17 cells, we analyzed transcriptome of versus CCR6– exposed inducer retinoic acid (RA) and performed functional validations colon biopsies HIV-infected individuals receiving ART (HIV+ART). Although...
Many debilitating infections result from persistent microbial biofilms that do not respond to conventional antibiotic regimens. A potential method treat such chronic is combine agents which interfere with bacterial biofilm development together an in a single formulation. Here, we explore the use of new bioresponsive polymer formulation derived specifically modified alginate nanoparticles (NPs) order deliver ciprofloxacin (CIP) combination quorum sensing inhibitor (QSI)...
Redox-responsive NPs, delivering DTX in combination with TUBB3 siRNA, increased activity lung cancer (LC) cells. After local administration LC mice models, NPs were retained into the lungs thus exerting high siRNA silencing efficacy.
Polycyclic aromatic hydrocarbons (PAHs) are widely distributed immunotoxic environmental contaminants well known to regulate expression of pro-inflammatory cytokines such as interleukine-1β and tumor necrosis factor-α. In the present study, we demonstrated that chemokine CCL1, notably involved in cardiovascular diseases inflammatory or allergic processes, constitutes a new molecular target for PAHs. Indeed, exposure PAHs benzo[a]pyrene (BP) markedly increased mRNA secretion CCL1 primary...
Detecting physiological levels of neurotransmitters in biological samples can advance our understanding brain disorders and lead to improved diagnostics therapeutics. However, neurotransmitter sensors for real-world applications must reliably detect low concentrations target analytes from small volume working samples. Herein, a platform robust ultrasensitive detection dopamine, an essential that underlies several disorders, based on graphene multitransistor arrays (gMTAs) functionalized with...
Triple negative breast cancer (TNBC) is one of the most difficult subtypes to treat, due its aggressiveness, high heterogeneity and lack targeted therapies. Efforts have been...
Regulation of genes targeted by the ligand-activated aryl hydrocarbon receptor (AhR) has been shown to be controlled calcium (Ca(2+)) changes induced AhR agonists such as environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The present study was designed characterize this link between Ca(2+) and pathway. We report that fast elevation intracellular in TCDD-exposed mammary MCF-7 cells associated with transient enhanced activity Ca(2+)/calmodulin (CaM)-dependent protein kinase...
Abstract Background We previously demonstrated that primary Th1Th17 cells are highly permissive to HIV-1, whereas Th1 relatively resistant. Molecular mechanisms underlying these differences remain unknown. Results Exposure replication competent and single-round VSV-G pseudotyped HIV strains provide evidence superior in vs. was regulated by located at entry post-entry levels. Genome-wide transcriptional profiling identified transcripts upregulated (n = 264) downregulated 235) (p-value <...
PEG-poly(caprolactone) co-polymers with disulfide-linked cores are highly efficient for delivery of the anti-cancer drug methotrexate <italic>in vitro</italic>.
The size, shape, and underlying chemistries of drug delivery particles are key parameters which govern their ultimate performance in vivo. Responsive desirable for triggered delivery, achievable through architecture change biodegradation to control vivo fate. Here, polymeric materials synthesized with linear, hyperbranched, star, micellar-like architectures based on 2-hydroxypropyl methacrylamide (HPMA), the effects 3D redox-responsive biological transport investigated. Variations "stealth"...
Combinations of the topoisomerase II inhibitor doxorubicin and poly (ADP-ribose) polymerase olaparib offer potential drug-drug synergy for treatment triple negative breast cancers (TNBC). In this study we performed in vitro screening combinations these drugs, administered directly or encapsulated within polymer nanoparticles, both 2D 3D spheroid models cancer. A variety assays were used to evaluate drug potency, calculations combination index (CI) values indicated that synergistic effects...
This study was designed to analyze the effects of Ca<sup>2+</sup>/calmodulin-dependent protein kinase (CaMKK) inhibitor STO-609 (7-oxo-7<i>H</i>-benzimidazo[2,1-<i>a</i>]benz[de]isoquinoline-3-carboxylic acid) toward aryl hydrocarbon receptor (AhR) pathway because (CaMK) Iα, known as a downstream CaMKK effector, has been recently shown contribute AhR cascade. failed alter up-regulation target CYP1A1 in response potent ligand 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin (TCDD) MCF-7 cells....
CD4+ T-cells from gut-associated lymphoid tissues (GALT) are major targets for HIV-1 infection. Recruitment of excess effector CD8+ in the proximity target cells is critical control viral replication. Here, we investigated colocalization potential HIV-specific and into GALT explored role retinoic acid (RA) regulating this process a cohort HIV-infected subjects with slow disease progression. The expression gut-homing molecules integrin β7, CCR6, CXCR3 was identified as "signature" but not...
New materials chemistries are urgently needed to overcome the limitations of existing biomedical in terms preparation, functionality and versatility, also regards their compatibility with biological environments. Here, we show that Passerini reactions especially suited for preparation drug delivery materials, as relatively few steps, polymers can be synthesized installed enabling conjugation encapsulation, self-assembly into micellar or vesicular architectures, facile attachment triggerable...
Abstract Amphiphilic block co‐polymers composed of poly(ethylene glycol)‐ co ‐poly(lactide)‐ ‐poly(2‐(( tert ‐butoxycarbonyl)amino)‐3‐propyl carbonate) (PEG‐pLA‐pTBPC) are synthesized in monomer ratios and arrangements to enable assembly into nanoparticles with different sizes architectures. These materials based on components clinical use, or known be biodegradable, retain the same fundamental chemistry across “AB” “BAB” In MCF7 MDA‐MB‐231 breast cancer cells, <100 nm internalized most...
A set of pilot filters packed with Zeolite filter media treated a quaternary ammonium chloride (QAC) were evaluated to verify the proof concept their combined antimicrobial capabilities. Escherichia coli was removed and inactivated most (2.83 log), followed by MS-2 (2.75 Klebsiella terriena (2.21 PRD-1 (1.95 Chlorella vulgaris (1.92 Cryptosporidium parvum oocysts (1.78 log). Especially, inactivation C. (1.19 log) higher than physical removal (0.54 The data suggest that QAC-treated in has...