A5086885946- HIV Research and Treatment
- Immune Cell Function and Interaction
- HIV-related health complications and treatments
- T-cell and B-cell Immunology
- Atherosclerosis and Cardiovascular Diseases
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Photodynamic Therapy Research Studies
- HIV/AIDS drug development and treatment
- Cytomegalovirus and herpesvirus research
- Hepatocellular Carcinoma Treatment and Prognosis
- Plant Virus Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Hepatitis B Virus Studies
- Porphyrin Metabolism and Disorders
- Cancer, Lipids, and Metabolism
- Data Mining Algorithms and Applications
- Systemic Lupus Erythematosus Research
- Nanoplatforms for cancer theranostics
- Monoclonal and Polyclonal Antibodies Research
- Curcumin's Biomedical Applications
- Reproductive System and Pregnancy
- Liver Disease and Transplantation
- Immunodeficiency and Autoimmune Disorders
- Artificial Intelligence in Healthcare
Mansoura University
2016-2025
Centre Hospitalier de l’Université de Montréal
2016-2025
Sidi Mohamed Ben Abdellah University
2023-2024
Université de Montréal
2010-2023
Damietta University
2023
Alexandra Hospital
2023
Mubarak Al Kabeer Hospital
2023
Helwan University
2022
Orange Regional Medical Center
2021
Hôpital Saint-Luc
2007-2016
There is limited knowledge on the identity of primary CD4(+) T cell subsets selectively targeted by HIV-1 in vivo. In this study, we established a link between HIV permissiveness, phenotype/homing potential, and lineage commitment cells. CCR4(+)CCR6(+), CCR4(+)CCR6(-), CXCR3(+)CCR6(+), CXCR3(+)CCR6(-) cells expressed cytokines transcription factors specific for Th17, Th2, Th1Th17, Th1 lineages, respectively. CCR4(+)CCR6(+) CXCR3(+)CCR6(+) coreceptors CCR5 CXCR4 were permissive to R5 X4...
Abstract HIV persists in latently infected CD4 + T cells during antiretroviral therapy (ART). Immune checkpoint molecules, including PD-1, are preferentially expressed at the surface of persistently cells. However, whether PD-1 plays a functional role latency and reservoir persistence remains unknown. Using from HIV-infected individuals, we show that engagement inhibits viral production transcriptional level abrogates T-cell receptor (TCR)-induced reactivation Conversely, blockade with...
Chronic viral infections lead to persistent CD8 T cell activation and functional exhaustion. Expression of programmed death-1 (PD-1) has been associated dysfunction in HIV infection. Herein we report that another negative regulator activation, CD160, was also upregulated on HIV-specific lymphocytes mostly during the chronic phase cells expressed CD160 or PD-1 were still whereas co-expression defined a novel subset with all characteristics functionally exhausted cells. Blocking interaction...
Abstract Background Dicer, Ago2 and TRBP are the minimum components of human RNA-induced silencing complex (RISC). While Dicer RNases, is double-stranded RNA binding protein (dsRBP) that loads small interfering into RISC. binds directly to through its C-terminal domain. Results We show site in a 165 amino acid (aa) region located between ATPase helicase domains. The 69 aa domain, called C4, at end TRBP. TRBP1 TRBP2 isoforms, but not TRBPs lacking C4 (TRBPsΔC4), co-immunoprecipitated with...
HIV type 1 infection is associated with a rapid depletion of Th17 cells from the GALT. The chemokine receptor CCR6 marker for lineage polarization and permissiveness in memory CD4(+) T cells. CCR6(+) have potential to migrate into GALT via gut-homing integrin α(4)β(7), newly identified HIV-gp120 binding receptor. In this study, we investigated whether coexpressing β(7) are selective targets retinoic acid (RA)-induced imprinting selectively increases cell infection. We demonstrated that...
Background. Tryptophan (Trp) catabolism into kynurenine (Kyn) contributes to immune dysfunction in chronic human immunodeficiency virus (HIV) infection. To better define the relationship between Trp catabolism, inflammation, gut mucosal dysfunction, and role of early antiretroviral therapy (ART), we prospectively assessed patients after they acquired HIV.
Abstract The innate and adaptive immune systems fail to control HCV infection in the majority of infected individuals. is an ssRNA virus, which suggests a role for Toll-like receptors (TLRs) 7 8 initiating anti-viral response. Here we demonstrate that genomic RNA harbours specific sequences initiate anti-HCV response through TLR7 TLR8 various antigen presenting cells. Conversely, particles are detected by macrophages, but not monocytes DCs, TLR7/8 dependent mechanism; this leads chloroquine...
Early HIV-1 infection is characterized by enhanced tryptophan catabolism, which contributes to immune suppression and disease progression. However, the mechanism kynurenine, a tryptophan-related metabolite, induces remains poorly understood. Herein, we show that increased production of kynurenine correlates with defective interleukin-2 (IL-2) signaling in memory CD4 T cells from HIV-infected subjects. Defective IL-2 these subjects, drives reduced protection Fas-mediated apoptosis, was also...
Despite the success of antiretroviral therapy (ART), people living with HIV (PLWH) are still at higher risk for cardiovascular diseases (CVDs) that mediated by chronic inflammation. Identification novel inflammatory mediators inherent potential to be used as CVD biomarkers and also therapeutic targets is critically needed better stratification disease management in PLWH. Here, we investigated expression role multi-isoform proinflammatory cytokine IL-32 subclinical atherosclerosis PLWH (n=49...
Cardiovascular disease (CVD) remains an important comorbidity in people living with HIV-1 (PLWH) receiving antiretroviral therapy (ART). Our previous studies performed the Canadian HIV/Aging Cohort Study (CHACS) (>40 years-old; Framingham Risk Score (FRS) > 5%) revealed a 2–3-fold increase non-calcified coronary artery atherosclerosis (CAA) plaque burden, measured by computed tomography angiography scan (CTAScan) as total (TPV) and low attenuated volume (LAPV), ART-treated PLWH (HIV+)...
In chronic hepatitis C (CHC), the virus may induce autoimmune responses via autoantibodies production, including antinuclear antibodies (ANA). Former studies reported great ANA predisposition in CHC and these ANAs be related to worse prognosis hepatocellular carcinoma (HCC). We aimed evaluate association between incidence CHC-related HCC development molecules effect on severity tumor size advanced stages. Results revealed that seropositivity was associated with disease severity. patients...
People with HIV (PWH) have an increased risk of developing cardiovascular disease (CVD). Our recent data demonstrated that the multi-isoform proinflammatory cytokine IL-32 is upregulated in PWH and associated arterial stiffness subclinical atherosclerosis. However, mechanisms by which contributes to pathogenesis these diseases remain unclear. Here, we show while less expressed IL-32α isoform induces differentiation human classical monocytes into calcium-resorbing osteoclast cells, dominantly...
Abstract HIV-infected slow progressors (SP) represent a heterogeneous group of subjects who spontaneously control HIV infection without treatment for several years while showing moderate signs disease progression. Under conditions that remain poorly understood, subgroup these experience failure spontaneous immunological and virological control. Here we determined the frequency SP showed loss within our Canadian Cohort + Slow Progressors identified proinflammatory cytokine IL-32 as robust...
ABSTRACT The expression strategy of parvoviruses the Densovirus genus has as yet not been reported. Clones were obtained from densonucleosis virus Galleria mellonella ( Gm DNV) that yielded infectious upon transfection into LD652 cells. Its genome was found to be longest (6,039 nucleotides [nt]), with largest inverted terminal repeats (ITRs) (550 nt) among all parvoviruses. distal 136 nt could folded hairpins flop or flip sequence orientations. In contrast vertebrate parvoviruses, gene...
Background People living with HIV (PLWH) have a higher risk of myocardial infarction. Coronary atherosclerotic plaque CT characterization helps to predict cardiovascular risk. Purpose To measure characteristics coronary in PLWH without known disease and healthy volunteers HIV. Materials Methods In this prospective study, noncontrast (all participants, n = 265) was used for artery calcium (CAC) scoring asymptomatic HIV, disease, from 2012 2019. At angiography (n 233), prevalence, frequency,...
Abstract Background Chronic inflammation persists in some people living with human immunodeficiency virus (HIV) during antiretroviral therapy and is associated premature aging. The glycoprotein 120 (gp120) subunit of HIV-1 envelope sheds can be detected plasma, showing immunomodulatory properties even the absence detectable viremia. We evaluated whether plasma soluble gp120 (sgp120) a family gp120-specific anti–cluster A antibodies, linked to CD4 depletion vitro, contribute chronic...