A5010793898- 3D Printing in Biomedical Research
- Nanoparticle-Based Drug Delivery
- Advanced Polymer Synthesis and Characterization
- Dendrimers and Hyperbranched Polymers
- Cellular Mechanics and Interactions
- Microfluidic and Bio-sensing Technologies
- biodegradable polymer synthesis and properties
- Chemistry and Chemical Engineering
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- bioluminescence and chemiluminescence research
- Carbon dioxide utilization in catalysis
- HER2/EGFR in Cancer Research
- Photopolymerization techniques and applications
- Protein purification and stability
- Drug Solubulity and Delivery Systems
- Analytical Chemistry and Chromatography
University of Nottingham
2019-2023
AstraZeneca (United Kingdom)
2021
Park University
2019
The size, shape, and underlying chemistries of drug delivery particles are key parameters which govern their ultimate performance in vivo. Responsive desirable for triggered delivery, achievable through architecture change biodegradation to control vivo fate. Here, polymeric materials synthesized with linear, hyperbranched, star, micellar-like architectures based on 2-hydroxypropyl methacrylamide (HPMA), the effects 3D redox-responsive biological transport investigated. Variations "stealth"...
In order for synthetic polymers to find widespread practical application as biomaterials, their syntheses must be easy perform, utilising freely available building blocks, and should generate products which have no adverse effects on cells or tissue. addition, it is highly desirable that the synthesis platform biomaterials can adapted with a range of physical properties macromolecular architectures, multiple functional handles allow derivatisation 'actives' sensing therapy. Here we describe...
Abstract Biomechanical cues from the extracellular matrix (ECM) are essential for directing many cellular processes, normal development and repair, to disease progression. To better understand cell-matrix interactions, we have developed a new instrument named ‘OptoRheo’ that combines light sheet fluorescence microscopy with particle tracking microrheology. OptoRheo lets us image cells in 3D as they proliferate over several days while simultaneously sensing mechanical properties of...
Abstract Amphiphilic block co‐polymers composed of poly(ethylene glycol)‐ co ‐poly(lactide)‐ ‐poly(2‐(( tert ‐butoxycarbonyl)amino)‐3‐propyl carbonate) (PEG‐pLA‐pTBPC) are synthesized in monomer ratios and arrangements to enable assembly into nanoparticles with different sizes architectures. These materials based on components clinical use, or known be biodegradable, retain the same fundamental chemistry across “AB” “BAB” In MCF7 MDA‐MB‐231 breast cancer cells, <100 nm internalized most...
Recent developments in polymer synthesis methods allow the preparation of new materials across a wide chemical space and with high atom efficiency. In this work, we developed strategic approach...
Abstract The use of 1,8‐diazabicyclo[5.4.0]undec‐7‐ene (DBU) as a mild catalyst for the ring‐opening polymerization (ROP) pharma‐friendly and biodegradable monomer lactide functionalizable tert‐butyloxycarbonyl (BOC)‐protected cyclic carbonate is explored. Successful controlled ROP demonstrated when employing series labile‐ester (bis)(meth)acrylate initiators to produce macromonomers suitable range post‐polymerization modifications. Importantly, DBU ensured retention BOC group during...
Hyperbranched polymers have many promising features for drug delivery, owing to their ease of synthesis, multiple functional group content, and potential high loading with retention solubility. Here we prepared hyperbranched N-(2-hydroxypropyl)methacrylamide (HPMA) a range molar masses particle sizes, attached dyes, radiolabel or the anticancer gemcitabine. Reversible addition-fragmentation chain transfer (RAFT) polymerisation enabled synthesis pHPMA gemcitabine-comonomer functionalised...
Abstract Biomechanical cues from the extracellular matrix (ECM) are essential for directing many cellular processes, normal development and repair, to disease progression. To better understand cell-matrix interactions, we have developed a new instrument named ‘OptoRheo’ that combines light sheet fluorescence microscopy with particle tracking microrheology. OptoRheo lets us image cells in 3D as they proliferate over several days while simultaneously sensing mechanical properties of...
We present a bespoke new instrument for micro-mechanical sensing and imaging of 3D cell cultures. Our combines optical tweezers multiplane microrheology, with light sheet microscopy fluorescence imaging. This integrated system allows us to visualise cells in their environment while also mapping the physical properties extracellular matrix local cells. Such holistic approach will allow wide range fundamental questions life sciences be addressed by effectively micro-world from cell's perspective.
This talk will introduce a novel, recently developed, instrument that combines light sheet microscopy, optical trapping and multiplane imaging in single platform capable of 3D cultures live cell over long time courses mapping to these images the local mechanical properties material surrounding cells. The presentation highlight wide-range samples this can study discuss analysis involved evaluate rheological when either potential or are non-isotropic.