A5001947424- CAR-T cell therapy research
- Virus-based gene therapy research
- Single-cell and spatial transcriptomics
- Lymphoma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Hematological disorders and diagnostics
- Acute Myeloid Leukemia Research
- Chromosomal and Genetic Variations
- Cytomegalovirus and herpesvirus research
- Cancer Genomics and Diagnostics
- Immune cells in cancer
- Mental Health and Psychiatry
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Telomeres, Telomerase, and Senescence
- Bioinformatics and Genomic Networks
- RNA Interference and Gene Delivery
- Criminal Justice and Corrections Analysis
- Advanced biosensing and bioanalysis techniques
- Mental Health and Patient Involvement
- Psychiatric care and mental health services
- interferon and immune responses
- Solar and Space Plasma Dynamics
- Genomics and Phylogenetic Studies
University of Southern California
2017-2024
Coventry University
2007-2023
Cedars-Sinai Medical Center
2019-2023
Cancer Institute (WIA)
2022
Whittier College
2021
De la Préhistoire à l'Actuel : Culture, Environnement et Anthropologie
2020
Keck Hospital of USC
2016
University of Warwick
1998-2005
University of Bergamo
1992-2000
Abstract Hodgkin lymphoma is characterized by an extensively dominant tumor microenvironment (TME) composed of different types noncancerous immune cells with rare malignant cells. Characterization the cellular components and their spatial relationship crucial to understanding cross-talk therapeutic targeting in TME. We performed single-cell RNA sequencing more than 127,000 from 22 tissue specimens 5 reactive lymph nodes, profiling for first time phenotype lymphoma–specific at resolution....
Abstract Multiplexed immune cell profiling of the tumor microenvironment (TME) in cancer has improved our understanding immunology, but complex spatial analyses tumor-immune interactions lymphoma are lacking. Here, we used imaging mass cytometry (IMC) on 33 cases diffuse large B-cell (DLBCL) to characterize and architecture correlate it clinicopathological features such as origin, gene mutations, responsiveness chemotherapy. To understand poor response DLBCL checkpoint inhibitors (ICI),...
About a third of patients with relapsed or refractory classic Hodgkin lymphoma (r/r CHL) succumb to their disease after high-dose chemotherapy followed by autologous stem-cell transplantation (HDC/ASCT). Here, we aimed describe spatially resolved tumor microenvironment (TME) ecosystems establish novel biomarkers associated treatment failure in r/r CHL.
Abstract Genomic transposable elements (TEs) comprise nearly half of the human genome. The expression TEs is considered potentially hazardous, as it can lead to insertional mutagenesis and genomic instability. However, recent studies have revealed that are involved in immune-mediated cell clearance. Hypomethylating agents increase cancer cells, inducing ‘viral mimicry’, causing interferon signalling killing. To investigate role pathogenesis acute myeloid leukaemia (AML), we studied TE...
Senescent cells constitutively secrete inflammatory cytokines, known as the senescence-associated secretory phenotype (SASP). Previous work has implicated SASP in immune-mediated clearance of senescent cells; however, its regulation remains unknown. Our recent transcriptome profiling study shown that human stem and progenitors (s-HSPCs) robustly express genomic transposable elements (TEs) pathways inflammation. Furthermore, hypomethylating agents have been previously to induce expression TEs...
Over half of the human genome is comprised transposable elements (TE). Despite large-scale studies transcriptome in cancer, a comprehensive look at TE expression and its relationship to various mutations or prognosis has not been performed. We characterized 178 adult acute myeloid leukemia (AML) patients using data from The Cancer Genome Atlas. mutation specific dysregulation multivariate linear model. identified distinct patterns associated with transcriptional networks. Genes regulating...
In the present study salivary IgA, anti-Escherichia coli, anti-beta-lactoglobulin and anti-poliovirus type 1 IgA IgM in serum saliva were evaluated longitudinally 13 breast-fed 14 formula-fed infants over first six months of life. Salivary was quantified by electroimmunodiffusion; specific antibodies determined ELISA. significantly lower at age one month compared with but increased higher than month. both groups infants, months, levels adult controls. No significant differences secretory...
Aging is associated with complex molecular alterations at the cellular level. Bone marrow exhibits distinct phenotypic, genetic and epigenetic aging. Metabolic changes in bone related to aging have not been studied. In this study, we characterized metabolome transcriptome of murine compared it from young healthy mice chemotherapy treated mice; treatment known induce age-related hematopoiesis. The exhibited a signature suppressed fatty-acid oxidation: accumulation free fatty acids, reduced...
We evaluated the immunogenicity and antigenicity of a formula based on partially hydrolyzed cow's milk whey protein in infants at risk atopy controls. Total IgE specific IgE, IgG, IgG4 subclass antibodies against egg albumin α‐lactalbumin, casein, β‐lactoglobulin were measured by radioimmunoassay cord blood peripheral 5 days 6 months life five groups infants: 16 breast‐fed (group 1), 21 formula‐fed 2), 14 3), 10 control 4), 13 5). concentration was significantly lower group 2 than 3 similar...
This paper employs the methodological framework of linguistic analytic philosophy to explore conceptual issues arising from a study (published by us elsewhere) different models disorder implicit in five groups stakeholders concerned community care people with diagnosis long-term schizophrenia (users/consumers, carers, mental health social workers, psychiatrists and psychiatric nurses). Linguistic analysis, (1) gives precise fix on nature practical difficulties presented such (as use rather...
Genomic transposable elements (TEs) constitute the majority of genome. Expression TEs is known to activate double-stranded RNA recognition pathway ("viral mimicry"), leading activation interferon-stimulated genes, inflammation, and immune-mediated cell death. Recently, we showed that expression suppressed along with immune pathways in leukemic stem cells (LSCs) acute myeloid leukemia, suggesting a potential mechanism for escape LSCs. This indicated that, during oncogenesis, where there from...
Abstract Purpose: Our preclinical studies showed that the oncolytic reovirus formulation pelareorep (PELA) has significant immunomodulatory anti-myeloma activity. We conducted an investigator-initiated clinical trial to evaluate PELA in combination with dexamethasone (Dex) and bortezomib (BZ) define tumor immune microenvironment (TiME) patients multiple myeloma treated this regimen. Patients Methods: relapsed/refractory (n = 14) were enrolled a phase Ib (ClinicalTrials.gov: NCT02514382) of...
<ns3:p>The recently introduced Kallisto pseudoaligner has radically simplified the quantification of transcripts in RNA-sequencing experiments. We offer cloud-scale RNAseq pipelines <ns3:italic>Arkas-Quantification</ns3:italic>, and <ns3:italic>Arkas-Analysis </ns3:italic>available within Illumina’s BaseSpace cloud application platform which expedites preparatory routines, reliably calculates differential expression, performs gene-set enrichment REACTOME pathways<ns3:italic>....
<ns4:p>The recently introduced Kallisto pseudoaligner has radically simplified the quantification of transcripts in RNA-sequencing experiments. We offer cloud-scale RNAseq pipelines <ns4:italic>Arkas-Quantification</ns4:italic>, which deploys for parallel cloud computations, and <ns4:italic>Arkas-Analysis,</ns4:italic> annotates results by extracting structured information directly from source FASTA files with per-contig metadata calculates differential expression gene-set enrichment...
The government's social exclusion unit (SEU) was established to help individuals, groups, and regions overcome deprivation discrimination resulting from a combination of problems, including unemployment, poor quality housing, low income, lack education/training opportunities, bad health, family breakdown. Such difficulties are commonly experienced by people with mental health problems who also have cope in society which alienates rejects them, barring them every aspect community life....
Senescence, a state of permanent cell cycle arrest, can be induced by DNA damage. This process, which was initially described in fibroblasts, is now recognized to occur stem cells. It has been well characterized lines, but there currently very limited data available on human senescence vivo. We recently reported that the expression transposable elements (TE), including endogenous retroviruses, up-regulated along with inflammatory genes senescent hematopoietic and progenitor cells (HSPCs) The...
Abstract Diffuse large B cell lymphoma (DLBCL) is an aggressive and heterogenous entity characterized by its variable clinical biological behaviour, approximately 30% of patients experience relapsed or refractory disease after first-line therapy. We hypothesize that a better characterization the tumor microenvironment (TME) might help identify who may benefit from individualized immunotherapies. Similar studies in this area have been limited technical challenges - conventional highly...
Abstract Classic Hodgkin Lymphoma (CHL) is a B-cell lymphoma characterized by Reed- Sternberg (HRS) cells surrounded immune cell infiltrates that define histological subtypes. A subset of CHL tumors shows integration the Epstein-Barr virus (EBV) genome into HRS cell. EBV tumor positivity thought to reflect immunocompromised status and has been more common in young older patients, non-white patients lower socioeconomic status. This study aims examine distribution prevalence determine whether...
Abstract Background: African American (AA) men are more than twice as likely to die of prostate cancer (PC) compared non-Hispanic White in the US. Among AA men, we examined survival after PC diagnosis by neighborhood-level structural racism and whether its effects mediated neighborhood socioeconomic status (nSES). Methods: We pooled data for 133,241 diagnosed with from 2000-2013 ten population-based registries across eight states (CA, Detroit, FL, GA, LA, NJ, NY, TX) RESPOND Study (Research...
Keywords values, values-based practice, models of disorder, concept mental illness, user-centred patient-centred multidisciplinary teamwork We are grateful to our four commentators for putting much-needed conceptual air and space around the project. Published originally as an empirical study different implicit held by users services, carers, nurses, psychiatrists, social workers (Colombo et al. 2003), paper outlined philosophical roots project in linguistic analytic philosophy "Oxford...
Multi-agency collaboration is a central concept of the rapidly expanding public protection agenda. But can services, working to different agendas, ever get along? Tim Turner and Anthony Colombo discuss new national strategy that aims promote flow information