A5105408392- Biochemical and Molecular Research
- Cell Adhesion Molecules Research
- Cytomegalovirus and herpesvirus research
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- TGF-β signaling in diseases
- Sphingolipid Metabolism and Signaling
- DNA and Nucleic Acid Chemistry
- HIV/AIDS drug development and treatment
- Genomics and Chromatin Dynamics
- Receptor Mechanisms and Signaling
- DNA Repair Mechanisms
- Connective tissue disorders research
- Genomics and Phylogenetic Studies
- Heat shock proteins research
- Glycosylation and Glycoproteins Research
- Animal Genetics and Reproduction
- Advanced biosensing and bioanalysis techniques
- Genetics and Neurodevelopmental Disorders
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Cholinesterase and Neurodegenerative Diseases
- Physiological and biochemical adaptations
- Phosphodiesterase function and regulation
Google (United States)
2017
University of Southern Denmark
2014-2015
Norwegian Institute of Bioeconomy Research
2011
Joint Genome Institute
2001-2002
Lawrence Livermore National Laboratory
1991-2001
California State University, Stanislaus
1993
University of Freiburg
1992
Stanford University
1992
Stanford Medicine
1992
University of Oxford
1992
The human genome holds an extraordinary trove of information about development, physiology, medicine and evolution. Here we report the results international collaboration to produce make freely available a draft sequence genome. We also present initial analysis data, describing some insights that can be gleaned from sequence.
Hypoxanthine-guanine phosphoribosyltransferase from Chinese hamster brain, liver, and V79 tissue culture cells appears to have identical structural catalytic properties.The enzyme has been purified 540-fold apparent homogeneity brain.The native molecular weight is 78,000 85,000 determined by Sephadex G-100 column chromatography acrylamide gel electrophoresis.The consist of three subunits 25,000 sodium dodecyl sulfate electrophoresis.Electrofocusing electrophoresis demonstrate the presence at...
Ceramide and its metabolites constitute a diverse group of lipids, which play important roles as structural entities biological membranes well regulators cellular growth, differentiation, development. The C. elegans genome comprises three ceramide synthase genes; hyl-1, hyl-2, lagr-1. HYL-1 function is required for synthesis ceramides sphingolipids containing very long acyl-chains (≥C24), while HYL-2 shorter (≤C22). Here we show that functional loss decreases lifespan, or LAGR-1 does not...
Abstract. It has recently become apparent that several cytokines and growth factors are capable of modulating fibroblast proliferation biosynthetic activity. To understand the role these in connective tissue regulation, we examined effects simultaneous addition interferon‐γ (IFN‐γ) transforming factor‐β (TGF‐β) on normal human dermal collagen fibronectin production. In addition, vitro transcription rates steady‐state mRNA levels for molecules were determined by nuclear run‐off assays...
Ceramides are precursors of complex sphingolipids (SLs), which important for normal functioning both the developing and mature brain. Altered SL levels have been associated with many neurodegenerative disorders, including epilepsy, although few direct links identified between genes involved in metabolism epilepsy.We used quantitative real-time PCR, Western blotting, enzymatic assays to determine mRNA, protein, activity ceramide synthase 2 (CERS2) fiibroblasts isolated from parental control...
Abstract. It has recently become apparent that several cytokines and growth factors are capable of modulating fibroblast proliferation biosynthetic activity. To understand the role these in connective tissue regulation, we examined effects simultaneous addition interferon‐γ (IFN‐γ) transforming factor‐β (TGF‐β) on normal human dermal collagen fibronectin production. In addition, vitro transcription rates steady‐state mRNA levels for molecules were determined by nuclear run‐off assays...
Abstract A minigene version of the human gene for pro alpha(I) chain type I procollagen (COL1A1) was prepared that contained -2.3 kilobases 5'-flanking sequence, first 5 exons and introns, last 6 about 2 3'-flanking sequence. The then used stable transfection experiments with mouse NIH 3T3 fibroblasts. Because products were shorter, it possible to compare expression endogenous alpha 1 (I) by Northern Western blot analyses. results demonstrated construct enough obtain high levels in many...
Hypoxanthine phosphoribosyltransferase (IMP:pryophosphate phosphoribosyltransferase, EC 2.4.2.8) from human erythrocytes has been purified 13 000-fold to apparent homogeneity. The native enzyme a sedimentation coefficient of 5.9 S, determined by analytical ultracentrifugation, and molecular weight 81 000-83 000, equilibrium centrifugation. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicates subunit 26 suggesting that the is trimer. Isoelectric focusing resolves three peaks...
To investigate this difference the enzyme was purified from human erythrocytes by method reported to give a 34,500 molecular weight subunit.Although purification produces what appears be single active band on nondenaturing polyacrylamide gels, major protein components observed sodium dodecyl sulfate poly acrylamide gels are not derived enzyme.Instead, activity is associated with minor component of subunit 26,000.Heating at 85' in presence phosphoribosylpyrophosphate yields complete recovery...
The HSPDE4A gene spans 50 kb, consists of at least 17 exons and is orientated 5´–3´, telomere to centromere. It located chromosome 19p13.2, being 350 kb proximal the encoding TYK2 850 distal low-density lipoprotein receptor. Its structure consistent with production active ‘long ’ ‘short isoenzymes as result alternative mRNA splicing two splice junctions. Identified single alternatively spliced 5´ exon unique N-terminal region long isoenzyme HSPDE4A4B (pde46). upstream conserved regions, UCR1...
A basic ribosomal phosphoprotein of 30,000 molecular weight was rapidly dephosphorylated in cultured Drosophila melanogaster cells heat shocked at 37 degrees C. The protein associated with the 40S subunit and had an electrophoretic mobility similar to that purified rat liver S6 on two-dimensional polyacrylamide gels as well a partial proteolysis peptide map. In logarithmically growing cultures, this D. appeared have single phosphorylated species consisting 30 40% total cellular S6. Thus,...
The carcinoembryonic antigen (CEA)-like genes are members of a large gene family which Is part the immunoglobulln superfamily.The CEA is divided into two major subgroups, CEA-subgroup and pregnancy-specific glycoprotein (PSG)-subgroup.In course an effort to develop set overlapping cosmlds spanning human chromosome 19, we identified 245 cosmids In 19 cosmid library (6 -7X redundant) by hybridization with IgC-like domain fragment gene.A fluorescence-based restriction enzyme digest...
Hypoxanthine phosphoribosyltransferase (HPRT, IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) can be purified 5-to 10,000-fold from extracts of HeLa (human) cells by a three-step procedure consisting high-speed centrifugation, adsorption to Sepharose-conjugated HPRT antibody, and sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Purified enzyme labeled in vivo with radioactive lysine, arginine, or methionine was digested trypsin the tryptic peptides were separated column...
We have developed an in vitro translation system from heat-shocked and normal Drosophila cultured cells.The lysates retain regulation of typical the whble cells which they were prepared, both when programmed by endogenous mRNA RNA-dependent.These systems been used to investigate mechanism shutdown protein synthesis selection heat shock mRNAs for Drosophila.Supplementation intact RNA-dependent with separated ribosome or supernatant fractions showed fraction cou1d"rescue" a lysate.Normal...