A5073953803- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Wastewater Treatment and Nitrogen Removal
- Chromosomal and Genetic Variations
- Genomic variations and chromosomal abnormalities
- Gut microbiota and health
- Genetic Neurodegenerative Diseases
- Genomics and Chromatin Dynamics
- T-cell and B-cell Immunology
- Neurogenetic and Muscular Disorders Research
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Toxic Organic Pollutants Impact
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Genetics and Neurodevelopmental Disorders
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Neuroblastoma Research and Treatments
- RNA Research and Splicing
- Immune Cell Function and Interaction
- Clostridium difficile and Clostridium perfringens research
- Microbial bioremediation and biosurfactants
- Microbial Fuel Cells and Bioremediation
- Fungal and yeast genetics research
Génomique Métabolique du Genoscope
2008-2022
Genoscope
2011-2021
Centre National de la Recherche Scientifique
2012-2021
Université Paris-Saclay
2018-2021
CEA Paris-Saclay
2019-2021
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2012-2021
Université d'Évry Val-d'Essonne
2005-2018
Direction de la Recherche Fondamentale
2016-2018
Centre National de Recherche en Génomique Humaine
2010-2015
University of Copenhagen
2011
To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe Illumina-based metagenomic sequencing, assembly characterization 3.3 million non-redundant microbial genes, derived from 576.7 gigabases sequence, faecal samples 124 European individuals. The gene set, ∼150 times larger than complement, contains an overwhelming majority prevalent (more frequent) genes cohort probably includes a large proportion intestinal...
A gene discovered by positional cloning has been identified as the von Hippel-Lindau (VHL) disease tumor suppressor gene. restriction fragment encompassing showed rearrangements in 28 of 221 VHL kindreds. Eighteen these were due to deletions candidate gene, including three large nonoverlapping deletions. Intragenic mutations detected cell lines derived from patients and sporadic renal carcinomas. The is evolutionarily conserved encodes two widely expressed transcripts approximately 6 6.5...
Summary The paradox of a host specificity the human faecal microbiota otherwise acknowledged as characterized by global functionalities conserved between humans led us to explore existence phylogenetic core. We investigated presence set bacterial molecular species that would be altogether dominant and prevalent within healthy humans. A total 10 456 non‐chimeric 16S rRNA sequences were obtained after cloning PCR‐amplified rDNA from 17 DNA samples. Using alignment or tetranucleotide...
Nitrospira are barely studied and mostly uncultured nitrite-oxidizing bacteria, which are, according to molecular data, among the most diverse widespread nitrifiers in natural ecosystems biological wastewater treatment. Here, environmental genomics was used reconstruct complete genome of “ Candidatus defluvii” from an activated sludge enrichment culture. On basis this first-deciphered experimental we show that Ca . N. defluvii differs dramatically other known nitrite oxidizers key enzyme...
Prior to constructing a library of yeast artificial chromosomes (YACs) containing very large human DNA fragments, we performed series preliminary experiments aimed at developing suitable protocol. We found an inverse relationship between YAC insert size and transformation efficiency. Evidence occasional rearrangement within inserts was resulting in clonally stable internal deletions or unstable variations. A protocol developed for preparative electrophoretic enrichment high molecular mass...
Abstract Nitrite-oxidizing bacteria (NOB) catalyze the second step of nitrification, a major process biogeochemical nitrogen cycle, but recognized diversity this guild is surprisingly low and only two bacterial phyla contain known NOB. Here, we report on discovery chemolithoautotrophic nitrite oxidizer that belongs to widespread phylum Chloroflexi not previously any nitrifying organism. This organism, named Nitrolancetus hollandicus, was isolated from reactor. Its tolerance broad temperature...
Many microorganisms live in anaerobic environments. Most of these have not yet been cultivated. Here, we present, from a metagenomic analysis an digester municipal wastewater treatment plant, reconstruction the complete genome bacterium belonging to WWE1 candidate division. In silico proteome indicated that this might derive most its carbon and energy fermentation amino acids, hence, it was provisionally classified as "Candidatus Cloacamonas acidaminovorans." acidaminovorans" is probably...
Spinal muscular atrophies (SMAs) represent the second most common fatal autosomal recessive disorder after cystic fibrosis. Childhood spinal are divided into severe (type I) and mild forms (types II III). By a combination of genetic physical mapping, yeast artificial chromosome contig 5q13 region spanning disease locus was constructed that showed presence low copy repeats in this region. Allele segregation analyzed at closest loci detected by markers C212 C272 201 SMA families. Inherited de...
Summary A culture‐independent molecular phylogenetic approach was used to study prokaryotic diversity in an anaerobic sludge digester. Two 16S rRNA gene libraries were constructed using total genomic DNA, and amplified by polymerase chain reaction (PCR) primers specific for archaeal or bacterial domains. Phylogenetic analysis of 246 579 almost full‐length genes Archaea Bacteria , respectively, performed the ARB software package. groups affiliated with belong Euryarchaeota Crenarchaeota ....