A5058004991- Enterobacteriaceae and Cronobacter Research
- Parasitic Infections and Diagnostics
- Listeria monocytogenes in Food Safety
- Plant Pathogenic Bacteria Studies
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Probiotics and Fermented Foods
- Microbial Inactivation Methods
- Microbial Community Ecology and Physiology
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Essential Oils and Antimicrobial Activity
- Wastewater Treatment and Nitrogen Removal
- Viral gastroenteritis research and epidemiology
- Biosensors and Analytical Detection
- Milk Quality and Mastitis in Dairy Cows
- Microbial infections and disease research
- Identification and Quantification in Food
- Spectroscopy and Chemometric Analyses
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Fecal contamination and water quality
- Veterinary Oncology Research
- Water Treatment and Disinfection
- Zoonotic diseases and public health
University of Zurich
2015-2025
Institute for Food Safety and Health
2016
University of Vienna
1995-2006
Technical University of Munich
2002-2005
Schott (Germany)
2004
University of Veterinary Medicine Vienna
1999-2001
University of Veterinary Medicine
2001
For cultivation-independent detection of sulfate-reducing prokaryotes (SRPs) an oligonucleotide microarray consisting 132 16S rRNA gene-targeted probes (18-mers) having hierarchical and parallel (identical) specificity for the all known lineages (SRP-PhyloChip) was designed subsequently evaluated with 41 suitable pure cultures SRPs. The applicability SRP-PhyloChip diversity screening SRPs in environmental clinical samples tested by using from periodontal tooth pockets chemocline a...
[Enterobacter] sakazakii is an opportunistic pathogen that can cause infections in neonates. This study further clarifies the taxonomy of isolates described as [E.] and completes formal description proposed reclassification these organisms novel species subspecies within a genus, Cronobacter gen. nov. was first defined 1980, however recent polyphasic taxonomic analysis has determined this group consists several genomospecies. In study, phenotypic descriptions are expanded using Biotype 100...
Abstract Background Enterobacter sakazakii is an opportunistic pathogen that can cause infections such as necrotizing enterocolitis, bacteraemia, meningitis and brain abscess/lesions. When the species was defined in 1980, 15 biogroups were described it suggested these could represent multiple species. In this study taxonomic relationship of strains E. further investigated. Results Strains identified divided into separate groups on basis f-AFLP fingerprints, ribopatterns full-length 16S rRNA...
ABSTRACT For simultaneous identification of members the betaproteobacterial order “ Rhodocyclales ” in environmental samples, a 16S rRNA gene-targeted oligonucleotide microarray (RHC-PhyloChip) consisting 79 probes was developed. Probe design based on phylogenetic analysis available sequences from all cultured and as yet uncultured .” The multiple nested probe set evaluated for hybridization with gene PCR amplicons 29 reference organisms. Subsequently, RHC-PhyloChip successfully used...
ABSTRACT A new microarray method, the isotope array approach, for identifying microorganisms which consume a 14 C-labeled substrate within complex microbial communities was developed. Experiments were performed with small consisting of oligonucleotide probes targeting 16S rRNA ammonia-oxidizing bacteria (AOB). Total RNA extracted from pure culture Nitrosomonas eutropha grown in presence [ C]bicarbonate. After fluorescence labeling and hybridization, scanning all probe spots radioactivity...
ABSTRACT The iap gene encodes the protein p60, which is common to all Listeria species. A previous comparison of DNA sequences indicated conserved and species-specific portions. Based on these comparisons, a combination consisting only five different primers that allows specific detection differentiation species with single multiplex PCR subsequent gel analysis was selected. One primer derived from 3′ end for species; other four are monocytogenes , L. innocua grayi or three grouped ivanovii...
ABSTRACT Two different real-time quantitative PCR (RTQ-PCR) approaches were applied for PCR-based quantification of Staphylococcus aureus cells by targeting the thermonuclease ( nuc ) gene. Purified DNA extracts from pure cultures S. quantified in a LightCycler system using SYBR Green I. Quantification proved to be less sensitive (60 gene copies/μl) than fluorigenic TaqMan probe (6 copies/μl). Comparison and well-established ABI Prism 7700 SDS with probes revealed no statistically...
ABSTRACT Low-sulfate, acidic (approximately pH 4) fens in the Lehstenbach catchment Fichtelgebirge mountains Germany are unusual habitats for sulfate-reducing prokaryotes (SRPs) that have been postulated to facilitate retention of sulfur and protons these ecosystems. Despite low situ availability sulfate (concentration soil solution, 20 200 μM) conditions (soil solution pHs, approximately 4 5, respectively), upper peat layers soils from two (Schlöppnerbrunnen I II) this displayed...
This study extends current understanding of the genetic diversity among L. monocytogenes from various food products and processing environments. Application WGS-based strategies facilitated tracking this pathogen importance to human health along production chain while providing insights into pathogenic potential for some isolates recovered. These analyses enabled grouping selected three putative virulence categories according their genotypes with informing selection phenotypic assessment...
E. sakazakii is considered to be an opportunistic pathogen, implicated in food borne diseases causing meningitis or enteritis especially neonates and infants. Cultural standard identification procedures for include the observation of yellow pigmentation colonies a positive glucosidase activity. Up now, only one PCR system based on single available 16S rRNA gene sequence has been published identification. However, our hands preliminary evaluation this number target non-target strains showed...
A taxonomic reclassification of the neonatal pathogen Enterobacter sakazakii to consist five species within a new genus, "Cronobacter," has recently been proposed. The correct identification these organisms is important clinicians. Therefore, using 312 Enterobacteriaceae, including 210 "Cronobacter" strains, reliabilities biochemical and genetic confirmation tests were investigated. All isolates positive dnaG gluA PCR protocols, all expressed alpha-glucosidase activity. ID32E v3.0 identified...
Enterobacter sakazakii is considered an opportunistic food-borne pathogen, causing rare but significant illness especially in neonates. It has been proposed that the organism relatively resistant to osmotic and dry stress compared other species of Enterobacteriaceae group. To understand mechanisms involved response, 2-DE protein analysis coupled MALDI-TOF MS was employed investigate changes profiles E. cells response two different types (physical desiccation growth hyperosmotic media). In...
Cronobacter spp. are opportunistic food-borne pathogens that can cause severe and sometimes lethal infections in neonates. In some outbreaks, the sources of infection were traced to contaminated powdered infant formula (PIF) or utensils used for PIF reconstitution. this study, we investigated biofilm formation sakazakii strain ES5. To investigate genetic basis on abiotic surfaces, screened a library random transposon mutants ES5 reduced using polystyrene microtiter assay. Genetic...
Abstract Background Members of the genus Cronobacter are causes rare but severe illness in neonates and preterm infants following ingestion contaminated infant formula. Seven species have been described two genomes were subsequently published. In this study, we performed comparative genomics on eight strains , including six that sequenced (representing seven species) previously published, closed genomes. Results We identified characterized features associated with core pan genome an attempt...
Listeria monocytogenes, an emerging foodborne pathogen, can cause in the population at risk severe infections that are associated with high case fatality rates. A total of 93 L. monocytogenes strains isolated from different patients Switzerland July 2011 to September 2013 were further characterized. Septicemia was reported for 74.2% patients, meningitis 10.8%, and abortion 3.2%. The majority belonged serotype 1/2a (n=58) followed by 4b (n=28), 1/2b (n=5), 1/2c (n=2). represented 35...