A5013483392- Acute Myeloid Leukemia Research
- Acute Lymphoblastic Leukemia research
- Histone Deacetylase Inhibitors Research
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Hematopoietic Stem Cell Transplantation
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Immune cells in cancer
- Sphingolipid Metabolism and Signaling
- Genomics and Rare Diseases
- Metabolism and Genetic Disorders
- Protein Degradation and Inhibitors
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- PARP inhibition in cancer therapy
- Phagocytosis and Immune Regulation
- Astrophysics and Cosmic Phenomena
- Advanced biosensing and bioanalysis techniques
- Planarian Biology and Electrostimulation
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Cytokine Signaling Pathways and Interactions
- Cytomegalovirus and herpesvirus research
University of Toronto
2015-2025
Princess Margaret Cancer Centre
2020-2025
University Health Network
2013-2025
Laboratoire de Physique Nucléaire et de Hautes Énergies
2015-2024
Occupational Cancer Research Centre
2016-2024
Donnelly College
2019-2022
Société Nationale des Chemins de Fer Français (France)
2020
Sorbonne Université
2015
Université Paris Cité
2015
Centre National de la Recherche Scientifique
2015
To investigate miRNA function in human acute myeloid leukemia (AML) stem cells (LSC), we generated a prognostic LSC-associated signature derived from functionally validated subpopulations of AML samples. For one miRNA, miR-126, high bioactivity aggregated all vivo patient sample LSC activity into single sorted population, tightly coupling miR-126 expression to function. Through functional studies, was found restrain cell cycle progression, prevent differentiation, and increase self-renewal...
Many long‐lived species of animals require the function adult stem cells throughout their lives. However, transcriptomes in invertebrates and vertebrates have not been compared, consequently, ancestral regulatory circuits that control cell populations remain poorly defined. In this study, we used data from high‐throughput RNA sequencing to compare pluripotent planarians with human mouse embryonic cells. From a stringently defined set 4,432 orthologs shared between planarians, mice humans,...
Lifelong blood cell production is governed through the poorly understood integration of cell-intrinsic and -extrinsic control hematopoietic stem (HSC) quiescence activation. MicroRNAs (miRNAs) coordinately regulate multiple targets within signaling networks, making them attractive candidate HSC regulators. We report that miR-126, a miRNA expressed in early progenitors, plays pivotal role restraining cell-cycle progression vitro vivo. miR-126 knockdown by using lentiviral sponges increased...
High-throughput OMICs experiments generate signals for millions of entities (i.e. genes, proteins, metabolites or any measurable biological entity) in the cell. In an effort to summarize and explore these signals, expression results are examined context known pathways processes, through enrichment analysis a set processes that is significantly enriched. Due high redundancy annotation resources this often hundreds sets. To facilitate results, we have developed Enrichment Map app visualize...
Triple-negative breast cancer (TNBC) includes basal-like and claudin-low subtypes for which no specific treatment is currently available. Although the retinoblastoma tumor-suppressor gene (RB1) frequently lost together with TP53 in TNBC, it not directly targetable. There thus great interest identifying vulnerabilities downstream of RB1 that can be therapeutically exploited. Here, we determined combined inactivation murine Rb p53 diverse mammary epithelial cells induced claudin-low–like TNBC...
It is critical to understand how human quiescent long-term hematopoietic stem cells (LT-HSCs) sense demand from daily and stress-mediated cues then transition into bioenergetically active progeny differentiate meet these cellular needs. However, the demand-adapted regulatory circuits of early steps hematopoiesis are largely unknown. Here we show that lysosomes, sophisticated nutrient-sensing signaling centers, regulated dichotomously by transcription factor EB (TFEB) MYC balance catabolic...
Freshwater planarians are well known for their regenerative abilities. Less is how maintain spatial patterning in long-lived adult animals or they re-pattern tissues during regeneration. HOX genes good candidates to regulate planarian patterning, yet the full complement genomic clustering of has not been described, primarily because only a few have detectable by situ hybridization, and none given morphological phenotypes when knocked down RNAi. Because Schmidtea mediterranea (S....
Abstract Disease recurrence causes significant mortality in B-progenitor acute lymphoblastic leukemia (B-ALL). Genomic analysis of matched diagnosis and relapse samples shows often arising from minor subclones. However, why therapy eradicates some subclones while others survive progress to remains obscure. Elucidation mechanisms underlying these differing fates requires functional isolated Here, large-scale limiting dilution xenografting samples, combined with targeted sequencing, identified...
Recent studies indicate that cancer-associated fibroblasts (CAFs) are phenotypically and functionally heterogeneous. However, little is known about CAF subtypes, the roles they play in cancer progression, molecular mediators of “state.” Here, we identify a novel cell surface pan-CAF marker, CD49e, demonstrate two distinct states, distinguished by expression fibroblast activation protein (FAP), coexist within CD49e+ compartment high-grade serous ovarian cancers. We show for first time state...
Abstract Mitochondrial metabolites regulate leukaemic and normal stem cells by affecting epigenetic marks. How mitochondrial enzymes localize to the nucleus control cell function is less understood. We discovered that metabolic enzyme hexokinase 2 (HK2) localizes in haematopoietic cells. Overexpression of nuclear HK2 increases properties decreases differentiation, whereas selective knockdown promotes differentiation function. Nuclear localization phosphorylation-dependent, requires active...