A5101417598- RNA modifications and cancer
- Fibroblast Growth Factor Research
- Cancer Cells and Metastasis
- Lung Cancer Research Studies
- Cancer Research and Treatments
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Chronic Kidney Disease and Diabetes
- Ubiquitin and proteasome pathways
- Renal and related cancers
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Single-cell and spatial transcriptomics
- Genetic and Kidney Cyst Diseases
- Advanced Proteomics Techniques and Applications
- Hippo pathway signaling and YAP/TAZ
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Cell Image Analysis Techniques
University of Toronto
2013-2020
University at Buffalo, State University of New York
2019
Feinstein Institute for Medical Research
2019
Donald & Barbara Zucker School of Medicine at Hofstra/Northwell
2019
Recent studies indicate that cancer-associated fibroblasts (CAFs) are phenotypically and functionally heterogeneous. However, little is known about CAF subtypes, the roles they play in cancer progression, molecular mediators of “state.” Here, we identify a novel cell surface pan-CAF marker, CD49e, demonstrate two distinct states, distinguished by expression fibroblast activation protein (FAP), coexist within CD49e+ compartment high-grade serous ovarian cancers. We show for first time state...
Cell surface proteins have a wide range of biological functions, and are often used as lineage-specific markers. Antibodies that recognize cell antigens widely research tools, diagnostic markers, even therapeutic agents. The ability to obtain broad protein profiles would thus be great value in fields. There however currently few available methods for high-throughput analysis large numbers proteins. We describe here flow cytometry (HT-FC) platform rapid 363 antigens. Here we demonstrate HT-FC...
High-grade serous ovarian carcinoma (HGSC) is the most common and lethal subtype of gynecologic malignancy in women. The current standard treatment combines cytoreductive surgery chemotherapy. Despite efficacy initial treatment, patients develop cancer recurrence, 70% die within 5 years diagnosis. CA125 FDA-approved biomarker used clinic to monitor response but its impact on patient survival limited. New strategies for discovery HGSC biomarkers are urgently needed. Here, we describe a...
RNA-binding proteins (RBPs) are instrumental in the biochemical processing and physiological functioning of non-coding RNAs. Therefore, as interest RNAs continues to expand, refining techniques capable probing protein-RNA interactions will prove ever more valuable characterization these molecules. To identify bound by a given RBP, cross-linking immunoprecipitation (CLIP) its iterations have been widely utilized, but approaches can be complex, labor-intensive, time consuming. Here, we...
Abstract High grade serous ovarian carcinoma has been shown to be a highly heterogeneous disease. In vivo studies demonstrate that the tumor initiating frequency (TIF) varies substantially from case case. Nonetheless, subset remains rare population within epithelial compartment and can enriched using cell surface marker CD133 (Stewart et al., PNAS, 2011). The microenvironment may play role in supporting maintaining cells (TIC) through direct and/ or indirect cross talk. contribution of...
Abstract High grade serous ovarian cancer (HGSOC) is the most common and lethal subtype of cancer. Many cancers, including HGSOC, contain a “stromal” component comprised cancer-associated fibroblasts (CAFs), immune cells, blood vessels, as well their secreted factors extracellular matrix. This complex microenvironment plays significant role in promoting tumor growth, therapy resistance, invasion. The majority studies CAFs utilize cells cultured passaged vitro under crude conditions to...
SUMMARY Recent studies indicate that cancer-associated fibroblasts (CAFs) are phenotypically and functionally heterogeneous. However, little is known about CAF subtypes the roles they play in cancer progression. Here we identify characterize two coexist within high grade serous ovarian cancers: Fibroblast activation protein (FAP)-high (FH) CAFs resemble classical myofibroblast-type CAF, whereas FAP-low (FL) possesses a preadipocyte-like molecular signature. Patients with predominantly FH...
Abstract High grade serous ovarian cancer (HGSC) is typically diagnosed at an advanced stage and the vast majority of patients relapse die within 5 years diagnosis. Significant clinical problems in HGSC include wide-spread abdominal dissemination disease chemotherapy resistance. Cancer-associated fibroblasts (CAFs) have been shown to play a role promotion cell proliferation invasion, mediation To interrogate molecular properties CAFs we used fluorescence activated sorting isolate directly...