A5100371932- Adipose Tissue and Metabolism
- Dietary Effects on Health
- Circadian rhythm and melatonin
- Adipokines, Inflammation, and Metabolic Diseases
- Regulation of Appetite and Obesity
- Cancer Cells and Metastasis
- Cardiovascular Disease and Adiposity
- Diet and metabolism studies
- Single-cell and spatial transcriptomics
- Body Contouring and Surgery
- Pluripotent Stem Cells Research
- Digestive system and related health
- Hippo pathway signaling and YAP/TAZ
- Acupuncture Treatment Research Studies
- Cancer-related molecular mechanisms research
- Pancreatitis Pathology and Treatment
- RNA modifications and cancer
- Gastrointestinal motility and disorders
- Epigenetics and DNA Methylation
- Fibroblast Growth Factor Research
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Skin Protection and Aging
- Telomeres, Telomerase, and Senescence
- Microscopic Colitis
Hospital for Sick Children
1997-2024
University of Toronto
1997-2024
SickKids Foundation
1997-2014
Dongshin University
2013
Intermittent fasting (IF), a periodic energy restriction, has been shown to provide health benefits equivalent prolonged or caloric restriction. However, our understanding of the underlying mechanisms IF-mediated metabolic is limited. Here we show that isocaloric IF improves homeostasis against diet-induced obesity and dysfunction primarily through adipose thermogenesis in mice. IF-induced require fasting-mediated increases vascular endothelial growth factor (VEGF) expression white tissue...
Abstract Stomach and intestinal stem cells are located in discrete niches called the isthmus crypt, respectively. Recent studies have demonstrated a surprisingly conserved role for Wnt signaling gastrointestinal development. Although stromal secrete ligands to promote cell renewal, source of stomach is still unclear. Here, by performing single analysis, we identify populations with transcriptome signatures that between intestine. In close proximity epithelial cells, these perictye-like...
Abstract The immunogenicity of transplanted allogeneic cells and tissues is a major hurdle to the advancement cell therapies. Here we show that overexpression eight immunomodulatory transgenes ( Pdl1 , Cd200 Cd47 H2-M3 Fasl Serpinb9 Ccl21 Mfge8 ) in mouse embryonic stem (mESCs) sufficient immunologically ‘cloak’ as well derived from them, allowing their survival for months outbred inbred recipients. Overexpression human orthologues these genes ESCs abolished activation peripheral blood...
Intermittent fasting (IF) is a widely practiced dietary method that encompasses periodic restriction of food consumption. Due to its protective benefits against metabolic diseases, aging, and cardiovascular neurodegenerative IF continues gain attention as preventative therapeutic intervention counteract these chronic diseases. Although numerous animal studies have reported positive health IF, feasibility efficacy in clinical settings remain controversial. Importantly, since interventions...
Abstract Intermittent fasting (IF) is an effective dietary intervention to counteract obesity-associated metabolic abnormalities. Previously, we and others have highlighted white adipose tissue (WAT) browning as the main underlying mechanism of IF-mediated benefits. However, whether IF retains its efficacy in different models, such genetically obese/diabetic animals, unknown. Here, leptin-deficient ob/ob mice were subjected 16 weeks isocaloric IF, comprehensive phenotyping was conducted...
The circadian clock regulates metabolism in anticipation of regular changes the environment. It is found throughout body, including key metabolic organs such as liver, adipose tissues, and intestine, where timing set largely by nutrient signaling. However, clocks these tissues during fasted state have not been completely characterized. Moreover, sufficiency a functioning host to produce diurnal rhythms composition microbiome animals has explored. To this end, mice were 24 h prior collection...
Adipose tissue is an important metabolic organ with high plasticity and responsive to environmental stimuli nutrient status. As such, various techniques have been developed study the morphology biology of adipose tissue. However, conventional visualization methods are limited studying in 2D sections, failing capture 3D architecture whole organ. Here we present whole-mount staining, immunohistochemistry method that preserves intact minimal processing steps. Hence, structures adipocytes other...
The increased prevalence of obesity and metabolic diseases has heightened interest in adipose tissue biology its potential as a therapeutic target. To better understand cellular heterogeneity complexity white (WAT), we employed cytometry by time-of-flight (CyTOF) to characterize immune stromal cells visceral subcutaneous WAT depots under normal high-fat diet feeding, quantifying the expression levels 32 surface marker proteins. We observed comparable proportions two steady state, but...
Intermittent fasting (IF), a dietary intervention involving periodic energy restriction, has been considered to provide numerous benefits and counteract metabolic abnormalities. So far, different types of IF models with varying durations feeding periods have documented. However, interpreting the outcomes is challenging, as many these involve multifactorial contributions from both time- calorie-restriction strategies. For example, alternate day model, often used rodent regimen, can result in...
Objectives : We studied the effects of electro and laser acupuncture treatment with GB39 GB34 on neuropathic pain in rats induced by tibial sural nerve ligation. Methods To produce model pain, nerves were ligated a 6-0 silk thread. Three days after surgery, only acupuncture(EA), 830 nm acupuncture(EA-LA-1), 904 acupuncture(EA-LA-2) treated twice week for 8 weeks. observed withdrawal response rats' legs von Frey filament acetone stimulation. also c-fos nocieptin central gray area midbrain...
Fibrosing colonopathy, a recently described complication of patients with cystic fibrosis, manifests clinically approximately 7–12 months after starting high dose pancreatic enzyme treatment. Although the pathogenesis fibrosing colonopathy is unknown, it highly correlated dose. In this study, immune mediated factors which may be associated were explored. Sera from 14 fibrosis and meconium ileus collected at diagnosis then longitudinally for four to five years analysed total IgG antiporcine...
<title>Abstract</title> The vagus nerve (VN) is a major component of the parasympathetic nervous system that regulates vital functions, including glucose and energy homeostasis. However, specific molecular signaling pathways within vagal neurons regulate metabolism are unclear. Here, we show enhanced PI3K through deletion its endogenous negative regulator, phosphatase tensin homolog (Pten), VN function along with decreased inflammation neurons. This was associated an anti-inflammatory state...
Intermittent fasting (IF), a dietary intervention involving periodic energy restriction, has been considered to provide numerous benefits and counteract metabolic abnormalities. So far, different types of IF models with varying durations feeding periods have documented. However, interpreting the outcomes is challenging, as many these involve multifactorial contributions from both time- calorie-restriction strategies. For example, alternate day model, often used rodent regimen, can result in...
The increased prevalence of obesity and metabolic diseases in the last few decades has heightened interest adipose tissue biology its potential as a therapeutic target. To better understand cellular heterogeneity complexity white (WAT), we employed cytometry by time-of-flight (CyTOF) identified various immune stromal cells visceral subcutaneous WAT depots under normal high-fat diet feeding, quantifying expression levels 32 surface marker proteins. We observed comparable proportions two...
Abstract White adipose tissue (WAT) fibrosis is a hallmark of dysfunctional WAT that directly linked to metabolic abnormalities. Recent studies have highlighted the role progenitor cells (APCs) in and impaired adaptive plasticity, leading systemic insulin resistance. However, therapeutic options for are limited. Intermittent fasting (IF) an effective dietary regimen weight control improvement through various mechanisms, including healthy remodeling WAT. whether IF improving age-associated...
Abstract Aging is a crucial factor for tissue fibrosis that closely associated with the functional decline of various organs. Fibrosis white adipose (WAT) hallmark dysfunctional WAT directly linked to age- and obesity-associated metabolic abnormalities. Recent studies have highlighted role progenitor cells (APCs) in impaired adaptive plasticity, leading systemic insulin resistance. However, therapeutic options are limited. Intermittent fasting (IF) an effective dietary regimen weight control...
Abstract The accumulation of senescent cells in white adipose tissue (WAT) is closely associated with the functional decline WAT and plays a causal role pathogenesis metabolic diseases. Therefore, elimination holds promise for treatment prevention age-related Using drug-repositioning strategy 2,150 clinically applied compounds, we discover that homoharringtonine (HHT), an FDA-approved anti-leukemic drug, manifests senotherapeutic activity human dermal fibroblasts murine preadipocytes vitro...
ABSTRACT White adipose tissue (WAT) fibrosis is a hallmark of dysfunctional WAT that directly linked to metabolic abnormalities. Recent studies have highlighted the role progenitor cells (APCs) in and impaired adaptive plasticity, leading systemic insulin resistance. However, therapeutic options for are limited. Intermittent fasting (IF) an effective dietary regimen weight control improvement through various mechanisms, including healthy remodeling WAT. whether IF improving age-associated...