A5048912470- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Antimicrobial Peptides and Activities
- Pain Mechanisms and Treatments
- Transcranial Magnetic Stimulation Studies
- IL-33, ST2, and ILC Pathways
- Pharmacological Effects of Natural Compounds
- Pancreatitis Pathology and Treatment
- Natural product bioactivities and synthesis
- Cell death mechanisms and regulation
- Fibroblast Growth Factor Research
- Blood disorders and treatments
- NF-κB Signaling Pathways
- Immune Response and Inflammation
- Muscle activation and electromyography studies
- Cellular Mechanics and Interactions
- Melanoma and MAPK Pathways
- Protein Hydrolysis and Bioactive Peptides
- Helicobacter pylori-related gastroenterology studies
- Spinal Cord Injury Research
- Neural dynamics and brain function
- Neurobiology and Insect Physiology Research
- Liver Disease Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
Burke Foundation
2024
Nanjing Medical University
2023
Nanjing General Hospital of Nanjing Military Command
2023
MIND Research Institute
2013-2023
Cornell University
2012-2023
Burke Medical Research Institute
2013-2023
Weill Cornell Medicine
2016-2023
Nanjing Agricultural University
2012-2023
White Plains Hospital
2022
New York Proton Center
2022
CAP18 (18-kDa cationic antimicrobial protein) is a protein originally identified and purified from rabbit leukocytes on the basis of its capacity to bind inhibit various activities lipopolysaccharide (LPS). Here we report cloning human characterize anti-LPS activity C-terminal fragment. Oligonucleotide probes designed cDNA were used identify bone marrow library. The encodes composed 30-amino-acid signal peptide, 103-amino-acid N-terminal domain unknown function, 37 amino acids homologous...
Intermittent fasting (IF), a periodic energy restriction, has been shown to provide health benefits equivalent prolonged or caloric restriction. However, our understanding of the underlying mechanisms IF-mediated metabolic is limited. Here we show that isocaloric IF improves homeostasis against diet-induced obesity and dysfunction primarily through adipose thermogenesis in mice. IF-induced require fasting-mediated increases vascular endothelial growth factor (VEGF) expression white tissue...
Recent evidence supports the concept that tumor growth in vivo depends on evasion of normal homeostatic control mechanisms operate through induction cell death by apoptosis. This study tested hypothesis a common property shared known or suspected promoters is ability to block process A total 10 were and all found inhibit DNA fragmentation 7 different lines triggered into apoptosis diverse agents. Resistance could be induced rapidly (within 1 h) treating with relatively high concentrations...
Recent evidence indicates that cell death through apoptosis may be an important mechanism to prevent tumor development. Therefore, agents inhibit function as promoters. The purpose of this study was determine the effects nicotine on process apoptosis. results demonstrate inhibits induced by diverse stimuli including necrosis factor (TNF), UV light, chemotherapeutic drugs, and calcium ionophore. This phenomenon observed in normal transformed cells derived from a variety species tissues, types...
Disrupted ERK1/2 (MAPK3/MAPK1) MAPK signaling has been associated with several developmental syndromes in humans; however, mutations ERK1 or ERK2 have not described. We demonstrate haplo-insufficient expression patients a novel approximately 1 Mb micro-deletion distal 22q11.2, region that includes ERK2. These exhibit conotruncal and craniofacial anomalies arise from perturbation of neural crest development defects comparable to the DiGeorge syndrome spectrum. Remarkably, these are replicated...
Blocking oxygen-sensing prolyl hydroxylases in the rodent CNS enhances functional recovery after brain hemorrhage.
Chronic itch, or pruritus, is associated with a wide range of skin abnormalities. The mechanisms responsible for chronic itch induction and persistence remain unclear. We developed mouse model in which constitutively active form the serine/threonine kinase BRAF was expressed neurons gated by sodium channel Nav1.8 (BRAFNav1.8 mice). found that constitutive pathway activation BRAFNav1.8 mice results ectopic enhanced expression cohort itch-sensing genes, including gastrin-releasing peptide...
Facilitating axon regeneration in the injured central nervous system remains a challenging task. RAF-MAP2K signaling plays key role elongation during development. Here, we show that conditional expression of constitutively kinase-activated BRAF mature corticospinal neurons elicited set transcription factors previously implicated zebrafish retinal ganglion cell axons and promoted sprouting tract (CST) after spinal cord injury mice. Newly collaterals formed synaptic connections with...
Interleukin-6 (IL-6) is a multifunctional cytokine mediating inflammatory or immune reactions. Here we investigated the possible role of IL-6 in intact lesioned peripheral nervous system using adult gene knockout (IL-6(-/-)) mice. Various sensory functions were tested by applying electrophysiological, morphological, biochemical, and behavioral methods. There was 60% reduction compound action potential branch IL-6(-/-) mice as compared with motor sciatic nerve. Cross sections L5 DRG showed...
Abstract Rabbit CAP18 (cationic antimicrobial protein, 18 kDa) is a leukocyte protein identified and purified using as an assay its capacity to bind inhibit various activities of LPS. Oligonucleotide probes designed from the putative N-terminal sequence were used obtain corresponding cDNA rabbit bone marrow library. Examination revealed that fragment N-terminus was actually 37-amino-acid C-terminal fragment. This fragment, designated CAP18(106-142), inhibits many In present studies,...
Activation of intrinsic growth programs that promote developmental axon may also facilitate regeneration in injured adult neurons. Here, we demonstrate conditional activation B-RAF kinase alone mouse embryonic neurons is sufficient to drive the long-range peripheral sensory projections vivo absence upstream neurotrophin signaling. We further show activated signaling enables robust regenerative axons into spinal cord after a dorsal root crush as well substantial regrowth crush-lesioned optic...
The NOD-like receptor family, pyrin domain-containing 3 (NLRP3) inflammasome plays pivotal roles in inflammation and autoimmunity. NLRP3 is activated response to various signals, including pathogen-associated molecular patterns (PAMPs) danger-associated (DAMPs). However, its role remains unclear. In this study, we used lipopolysaccharide (LPS) adenosine triphosphate (ATP) simulate an inflammatory environment as the testing model. We found that exposure of RAW264.7 cells LPS/ATP triggered...
We report the purification of a protease from tumor cells undergoing apoptosis that is involved in activating DNA fragmentation. Initial studies revealed two inhibitors serine proteases, N-1-tosylamide-2-phenylethylchloromethyl ketone and carbobenzoxy-Ala-Ala-borophe (DK120), suppressed necrosis factor or ultraviolet (UV) light-induced fragmentation U937 histiocytic lymphoma as well UV BT-20 breast carcinoma, HL-60 myelocytic leukemia, 3T3 fibroblasts. The was purified by affinity...
Pathogen-associated molecular patterns (PAMPs), moieties produced by invading microbial pathogens, initiate innate immune responses binding to pattern recognition receptors (PRRs). Engagement of PRRs elicits a wide variety responses, including the production and release cytokines chemokines. These require activation several parallel signaling pathways, NF-kappaB, interferon regulatory factors, MAPKs. The JNK p38 MAPK groups are major PRR effectors key PRR-dependent induction proinflammatory...