A5069460216- Cutaneous Melanoma Detection and Management
- Melanoma and MAPK Pathways
- Photoacoustic and Ultrasonic Imaging
- Protein Degradation and Inhibitors
- Nonmelanoma Skin Cancer Studies
- Histone Deacetylase Inhibitors Research
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Thermography and Photoacoustic Techniques
- CAR-T cell therapy research
- melanin and skin pigmentation
- Cutaneous lymphoproliferative disorders research
- Cancer Immunotherapy and Biomarkers
- Cancer-related gene regulation
- Protease and Inhibitor Mechanisms
- Skin Protection and Aging
- Nanoplatforms for cancer theranostics
- Polyomavirus and related diseases
- Ocular Oncology and Treatments
- Optical Imaging and Spectroscopy Techniques
- Dermatology and Skin Diseases
- Nail Diseases and Treatments
- Cancer Genomics and Diagnostics
- Cancer and Skin Lesions
- Diversity and Career in Medicine
Washington University in St. Louis
2016-2025
Barnes-Jewish Hospital
2000-2021
Council of Science Editors
2017-2021
Jewish Hospital
2000-2021
Cells Therapy (Poland)
2021
Bellingham Technical College
2015
In-Q-Tel
2014
Lynn University
2014
University Dermatology
2010
St. Louis Children's Hospital
2010
Abstract Angiostatin, a cleavage product of plasminogen, has been shown to inhibit endothelial cell proliferation and metastatic tumor growth. Recently, the production angiostatin correlated with tumor-associated macrophage elastolytic metalloproteinases in murine model Lewis lung carcinoma. In this report we demonstrate that purified human matrix generate biologically functional from plasminogen. Macrophage elastase (MMP-12 or MME) proved be most efficient angiostatin-producing MMP. MME was...
The continuous rise in skin cancer incidence highlights an imperative for improved prevention. Topical calcipotriol-plus-5-fluorouracil (calcipotriol-plus-5-FU) immunotherapy effectively eliminates precancerous lesions and prevents squamous cell carcinoma (SCC) patients. However, its mechanism of action remains unclear. Herein, we demonstrate that calcipotriol-plus-5-FU induces T helper type 2 (Th2) immunity, eliminating premalignant keratinocytes humans. CD4+ Th2 cells were required...
Abstract Matrix metalloproteinases (MMP) have been implicated in virtually all aspects of tumor progression. However, the recent failure clinical trials employing synthetic MMP inhibitors cancer chemotherapy has led us to hypothesize that some MMPs may actually serve host its defense against Here we show mice deficient macrophage elastase (MMP-12) develop significantly more gross Lewis lung carcinoma pulmonary metastases than their wild-type counterparts both spontaneous and experimental...
Systemic administration of IL-12p70 has demonstrated clinical activity in cancer patients, but dose-limiting toxicities have hindered its incorporation vaccine formulations. Here, we report on the immunological and outcomes upon vaccination with CD40L/IFN-γ-matured, IL-12p70-producing DCs.7 HLA-A*0201+ newly diagnosed stage IV melanoma patients were immunized against gp100 antigen using autologous peptide-pulsed, CD40L/IFN-γ-matured DCs. PBMCs taken weekly for immune monitoring by tetramer...
Actinic keratosis is a precursor to cutaneous squamous cell carcinoma. Long treatment durations and severe side effects have limited the efficacy of current actinic treatments. Thymic stromal lymphopoietin (TSLP) an epithelium-derived cytokine that induces robust antitumor immunity in barrier-defective skin. Here, we investigated calcipotriol, topical TSLP inducer, combination with 5-fluorouracil (5-FU) as immunotherapy for keratosis.The mechanism calcipotriol action against skin...
In several human volunteers, photoacoustic microscopy (PAM) has been utilized for noninvasive cutaneous imaging of the skin microvasculature and a melanocytic nevus. Microvascular networks in both acral nonacral were imaged, multiple features within have identified, including stratum corneum, epidermal-dermal junction, subpapillary vascular plexus. Several structural differences between also observed images. addition, nevus was photoacoustically excised, histologically analyzed. The images...
We developed handheld photoacoustic microscopy (PAM) to detect melanoma and determine tumor depth in nude mice vivo. Compared our previous PAM system for imaging, a new light delivery mechanism is introduced improve penetration. show that melanomas with 4.1 3.7 mm thicknesses can be successfully detected phantom vivo experiments, respectively. With its deep imaging ability design, this tested clinical diagnosis, prognosis, surgical planning patients at the bedside.
Chronic itch, or pruritus, is associated with a wide range of skin abnormalities. The mechanisms responsible for chronic itch induction and persistence remain unclear. We developed mouse model in which constitutively active form the serine/threonine kinase BRAF was expressed neurons gated by sodium channel Nav1.8 (BRAFNav1.8 mice). found that constitutive pathway activation BRAFNav1.8 mice results ectopic enhanced expression cohort itch-sensing genes, including gastrin-releasing peptide...
Topical calcipotriol plus 5-fluorouracil (5-FU) combination is an effective immunotherapy against actinic keratosis (AK), which a precursor to squamous cell carcinoma (SCC). However, the long-term effectiveness of 5-FU treatment for SCC prevention unknown.We performed blinded prospective cohort study on participants randomized double-blind clinical trial in 4-day course topical was compared Vaseline (control) AK treatment. and basal (BCC) incidences were assessed at 1, 2, 3 years after...
Melanoma is one of the most devastating malignancies with a rising incidence and lack effective treatments for advanced disease. Constitutive activation mitogen-activated protein kinase (MAPK) pathway altered expression alpha(v)beta(3) integrin are critical melanoma development progression. Ras-associated protein-1 (Rap1), Ras family member small GTPases, has emerged as key mediator in these two important processes. In this study, we have shown Rap1 cells derived from human metastatic...
The development of a novel BRAF inhibitor, vemurafenib, has been associated with impressive tumor regression in patients BRAF-positive stage IV melanoma. In the phase 3 clinical trials, dermatologic toxic effects vemurafenib were described, namely, eruptive squamous cell carcinomas. Herein, cases are presented that highlight carcinomas and other cutaneous sequelae have not previously reported reminiscent those observed administration multikinase inhibitor sorafenib tosylate. addition,...
To reveal the clonal architecture of melanoma and associated driver mutations, whole genome sequencing (WGS) targeted extension were used to characterize 124 cases. Significantly mutated gene analysis using 13 WGS cases 15 additional paired identified known genes such as BRAF, NRAS, CDKN2A, well a novel EPHA3, previously implicated in other cancer types. Extension studies tumors from another 96 patients discovered large number truncation mutations tumor suppressors (TP53 RB1), protein...
Microcirculation is an important component of the cardiovascular system and can be used to assess systemic health. Numerous studies have investigated cutaneous microcirculation as indicator related diseases. Such research has shown promising results; however, there are many limitations regarding employed measurement techniques, such poor depth spatial resolution versatility. Here we show results functional microvascular experiments measured with photoacoustic microscopy, which provides high...
Melanoma is the most serious, highly aggressive form of skin cancer with recent dramatic increases in incidence. Current therapies are relatively ineffective, highlighting need for a better understanding molecular mechanisms contributing to disease. We have previously shown that activation Rap1 promotes melanoma cell proliferation and migration through mitogen-activated protein kinase pathway integrin activation. In present study, we show expression Rap1GAP, specific negative regulator Rap1,...
We applied a linear‐array‐based photoacoustic probe to detect melanin‐containing melanoma tumor depth and volume in nude mice vivo . This system can image melanomas at five frames per second (fps), which is much faster than our previous handheld single transducer (0.1 fps). first theoretically show that, addition the higher frame rate, almost entire boundary of be detected by probe, while only horizontal could system. Then we demonstrate ability this measuring both through phantom, ex ,...
BACKGROUND The Sun Protection Outreach Teaching by Students (SPOTS) program previously demonstrated benefits to adolescents and medical students in the Midwest. Six years later, there have been significant enhancements geographic outreach has expanded. OBJECTIVE This multi-institutional evaluation study sought quantify efficacy of enhanced SPOTS improving skin cancer knowledge, sun-safe attitudes, sun protection behaviors living multiple regions United States. MATERIALS AND METHODS Surveys...