A5001405352- Retinal Diseases and Treatments
- Angiogenesis and VEGF in Cancer
- Glaucoma and retinal disorders
- Cell Adhesion Molecules Research
- Retinal Development and Disorders
- Retinopathy of Prematurity Studies
- Advanced Glycation End Products research
- Protein Interaction Studies and Fluorescence Analysis
- Retinal Imaging and Analysis
- Endodontics and Root Canal Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell death mechanisms and regulation
- Electrochemical sensors and biosensors
- Platelet Disorders and Treatments
- Barrier Structure and Function Studies
- Electrochemical Analysis and Applications
- Ubiquitin and proteasome pathways
- Corneal Surgery and Treatments
- Retinal and Optic Conditions
- Protease and Inhibitor Mechanisms
- Dental Radiography and Imaging
- Dental materials and restorations
- Proteoglycans and glycosaminoglycans research
- Hippo pathway signaling and YAP/TAZ
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
University of Wisconsin–Madison
2016-2025
Smith-Kettlewell Eye Research Institute
2015-2024
Pathways Behavioral Services
2017-2018
University of Tennessee Health Science Center
2015-2017
Madison Group (United States)
2016-2017
University of Tehran
2013-2016
McPherson College
2015-2016
Zhejiang University
2015
Second Affiliated Hospital of Zhejiang University
2015
Bioscience Research
2015
Disruption of the blood-brain barrier (BBB) occurs in many diseases and is often mediated by inflammatory neuroimmune mechanisms. Inflammation well established as a cause BBB disruption, but mechanistic questions remain.We used lipopolysaccharide (LPS) to induce inflammation disruption mice. was measured using (14)C-sucrose radioactively labeled albumin. Brain cytokine responses were multiplex technology dependence on cyclooxygenase (COX) oxidative stress determined treatments with...
The oxidative conversion of LDL into an atherogenic form is considered a pivotal event in the development cardiovascular disease. Recent studies have identified reactive nitrogen species generated by monocytes way myeloperoxidase-hydrogen peroxide-nitrite (MPO-H2O2-NO2–) system as novel mechanism for converting high-uptake (NO2-LDL) macrophages. We now identify scavenger receptor CD36 major responsible high-affinity and saturable cellular recognition NO2-LDL murine human Using cells stably...
Vitamin D compounds inhibit the growth of a variety tumors in preclinical and clinical studies. Among mechanisms suggested for this inhibition is antiangiogenesis. Retinal angiogenesis basis vision loss several major blinding diseases. The purpose study was to evaluate antiangiogenic activity calcitriol (1,25-dihydroxyvitamin D(3)) vivo its effect on retinal endothelial cell (EC) proliferation, migration, capillary morphogenesis vitro.The mouse oxygen-induced ischemic retinopathy (OIR) model...
The lack of capability to quantify oxygen metabolism noninvasively impedes both fundamental investigation and clinical diagnosis a wide spectrum diseases including all the major blinding such as age-related macular degeneration, diabetic retinopathy, glaucoma. Using visible light optical coherence tomography (vis-OCT), we demonstrated accurate robust measurement retinal metabolic rate (rMRO2) in rat eyes. We continuously monitored regulatory response consumption progressive hypoxic...
Quantitatively determining physiological parameters at a microscopic level in the retina furthers understanding of molecular pathways blinding diseases, such as diabetic retinopathy and glaucoma. An essential parameter, which has yet to be quantified noninvasively, is retinal oxygen metabolic rate (rMRO2). Quantifying rMRO2 challenging because two parameters, blood flow hemoglobin saturation (sO2), must measured together. We combined photoacoustic ophthalmoscopy (PAOM) with spectral...
Histological examination of abdominal aortic aneurysm (AAA) tissues demonstrates extracellular matrix destruction and infiltration inflammatory cells. Previous work with mouse models AAA has shown that anti-inflammatory strategies can effectively attenuate formation. Thrombospondin-1 is a matricellular protein involved in the maintenance vascular structure homeostasis through regulation biological functions, such as cell proliferation, apoptosis, adhesion. Expression levels thrombospondin-1...
Müller cells and macrophages/microglia are likely important for the development of diabetic retinopathy; however, interplay between these in this disease is not well understood. An inflammatory process linked to onset experimental retinopathy. CD40 deficiency impairs prevents Using mice with expression restricted cells, we identified a mechanism by which trigger proinflammatory cytokine myeloid cells. During diabetes, expressed upregulated retinal tumor necrosis factor-α (TNF-α), interleukin...
During inflammation polymorphonuclear neutrophils (PMNs) traverse venular walls, composed of the endothelium, pericyte sheath and vascular basement membrane. Compared to PMN transendothelial migration, little is known about how PMNs penetrate latter barriers. Using mouse models intravital microscopy, we show that migrating expand use low expression regions (LERs) matrix proteins in membrane (BM) for their transmigration. Importantly, demonstrate this remodeling LERs accompanied by opening...
Mesenchymal activation, characterized by dense stromal infiltration of immune and mesenchymal cells, fuels the aggressiveness colorectal cancers (CRC), driving progression metastasis. Targetable molecules in tumor microenvironment (TME) need to be identified improve outcome CRC patients with this aggressive phenotype. This study reports a positive link between high thrombospondin-1 (THBS1) expression characteristics, immunosuppression, unfavorable prognosis. Bone marrow-derived monocyte-like...
Murine endothelial cells are readily transformed in a single step by the polyomavirus oncogene encoding middle-sized tumor antigen. These (bEND.3) form tumors (hemangiomas) mice which lethal newborn animals. The bEND.3 rapidly proliferate culture and express little or no thrombospondin 1 (TS1). To determine role of TS1 regulation cell phenotype, we stably transfected with human expression vector. expressing were identified their altered morphology exhibited slower growth rate lower...
Abstract Thrombospondin‐1 (TSP1) is a natural inhibitor of angiogenesis. Its expression most prominent during the late stages vascular development and in adult vasculature. Our previous studies have shown that TSP1 promotes quiescent, differentiated phenotype endothelial cells. However, physiological role plays neovascularization requires further delineation. Here, we investigated retinal vasculature oxygen‐induced ischemic retinopathy. The density was increased TSP1‐deficient (TSP1‐/‐) mice...
Hyperglycemia impacts retinal vascular function and promotes the development progression of diabetic retinopathy, which ultimately results in growth new blood vessels loss vision. How high glucose affects endothelial cell (EC) properties requires further investigation. Here we determined impact on mouse EC vitro. High significantly enhanced migration without impacting their proliferation, apoptosis, adhesion, capillary morphogenesis. The under was reversed presence antioxidant...
The hypothesis that marrow-derived cells, and specifically proinflammatory proteins in those play a critical role the development of diabetes-induced retinopathy tactile allodynia was investigated. Abnormalities characteristic early stages were measured chimeric mice lacking inducible nitric oxide synthase (iNOS) or poly(ADP-ribosyl) polymerase (PARP1) only their cells. Diabetes-induced capillary degeneration, changes, superoxide production retina inhibited diabetic animals which iNOS PARP1...