A5100627212- Cell Adhesion Molecules Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Spine and Intervertebral Disc Pathology
- Spinal Fractures and Fixation Techniques
- Organoboron and organosilicon chemistry
- S100 Proteins and Annexins
- Barrier Structure and Function Studies
- Essential Oils and Antimicrobial Activity
- Piperaceae Chemical and Biological Studies
- Cytokine Signaling Pathways and Interactions
- Cervical and Thoracic Myelopathy
- Phytochemistry and biological activity of medicinal plants
- Genomics, phytochemicals, and oxidative stress
- Phytochemical compounds biological activities
- Musculoskeletal pain and rehabilitation
- Crystallography and molecular interactions
- Traditional Chinese Medicine Analysis
- Bioactive Compounds and Antitumor Agents
- Cholinesterase and Neurodegenerative Diseases
- Supramolecular Chemistry and Complexes
- Cellular Mechanics and Interactions
- Sirtuins and Resveratrol in Medicine
- Cancer, Hypoxia, and Metabolism
- Ocular and Laser Science Research
- Sulfur Compounds in Biology
Changzhi University
2021-2025
Capital Medical University
2024
Huazhong University of Science and Technology
2018-2023
Peking University
2008-2022
Yantaishan Hospital
2021-2022
Peking University First Hospital
2015-2022
Institute of Physics
2022
State Key Laboratory of Natural Medicine
2021
China Pharmaceutical University
2016-2021
Northwest A&F University
2016-2020
The mechanism of leukocyte migration through venular walls in vivo is largely unknown. By using immunofluorescence staining and confocal microscopy, the present study demonstrates existence regions within unstimulated murine cremasteric venules where expression key vascular basement membrane (BM) constituents, laminin 10, collagen IV, nidogen-2 (but not perlecan) are considerably lower (<60%) than average detected same vessel. These sites were closely associated with gaps between...
During inflammation polymorphonuclear neutrophils (PMNs) traverse venular walls, composed of the endothelium, pericyte sheath and vascular basement membrane. Compared to PMN transendothelial migration, little is known about how PMNs penetrate latter barriers. Using mouse models intravital microscopy, we show that migrating expand use low expression regions (LERs) matrix proteins in membrane (BM) for their transmigration. Importantly, demonstrate this remodeling LERs accompanied by opening...
Leukocyte recruitment from blood to inflammatory sites occurs in a multistep process that involves discrete molecular interactions between circulating and endothelial cells. Junctional adhesion molecule (JAM)-C is expressed at different levels on cells of lymphoid organs peripheral tissues has been proposed regulate neutrophil migration by its interaction with the leukocyte integrin Mac-1. In present study, we show accumulation leukocytes alveoli during acute pulmonary inflammation mice...
A catalytic, metal-free O–H bond insertion of α-diazoesters in water the presence B(C6F5)3·nH2O (2 mol %) was developed, affording a series α-hydroxyesters good to excellent yields. The reaction features easy operation and wide substrate scope, importantly, no metal is needed as compared with conventional methods. Significantly, this approach further expands applications B(C6F5)3 under water-tolerant conditions.
A series of novel benzo[c][1,2,5]oxadiazole derivatives were designed, synthesized, and biologically evaluated as inhibitors PD-L1. Among them, compound L7 exhibited 1.8 nM IC50 value in a homogeneous time-resolved fluorescence (HTRF) assay, which was 20-fold more potent than the lead BMS-1016. In surface plasmon resonance (SPR) bound to human PD-L1 (hPD-L1) with KD 3.34 nM, without showing any binding hPD-1. cell-based coculture blocked PD-1/PD-L1 interaction an EC50 375 while BMS-1016 had...
The heterogeneous nature of the perivascular basement membrane (composed primarily laminin and collagen type IV) suggests existence an elaborate array adhesive interactions possibly proteolytic events in leukocyte migration through this barrier. In context, blockade alpha6 integrins (laminin receptors), neutrophil elastase (NE) or both inhibited interleukin-1beta (IL-1beta)-stimulated mouse cremasteric venules, as observed by intravital microscopy. Furthermore, analysis tissues confocal...
Abstract In vitro and in vivo evidence supports a functional role for the integrin α6β1 neutrophil migration through perivascular basement membrane, response that appears to be associated with platelet/endothelial cell adhesion molecule-1 (PECAM-1)-mediated up-regulation of on surface transmigrating leukocytes. As involvement PECAM-1 leukocyte is cytokine-specific, aim present study was investigate whether exhibited similar profile stimulus specificity this context. The cytokines...
Percutaneous coronary intervention is the main therapy for revascularization of occluded arteries. However, a progressive artery restenosis caused by abnormal proliferation and migration vascular smooth muscle cells (VSMC) hinders effective treatment. In this study, we examined effect emodin, natural anthraquinoid compound, on cultured VSMC. Lower doses emodin suppressed cell induced unscheduled DNA synthesis. Higher increased lumpy chromatin condensation lysosomes in VSMC, suggesting...
A series of novel bivalent β-carboline derivatives were designed and synthesized, in vitro cytotoxicity, cell apoptosis, DNA-binding affinity evaluated. The cytotoxic results demonstrated that most exhibited stronger cytotoxicity than the corresponding monomer against five selected tumor lines (A549, SGC-7901, Hela, SMMC-7721, MCF-7), indicating dimerization at C³ position could enhance antitumor activity β-carbolines. Among tested, 4B, 6i, 4D, 6u displayed considerable A549 line....
As part of our continuing research on canthin-6-one antimicrobial agents, a new series ester derivatives 10-hydroxycanthin-6-one were synthesized using simple and effective synthetic route. The structure each compound was characterized by NMR, ESI-MS, FT-IR, UV, elemental analysis. activity these compounds against three phytopathogenic fungi (Alternaria solani, Fusarium graminearum, solani) four bacteria (Bacillus cereus, Bacillus subtilis, Ralstonia solanacearum, Pseudomonas syringae)...
B(C6F5)3·H2O has been long recognized as a common Brønsted acid. The lack of X-ray crystal structure with other substrates greatly limited the development new catalytic mode. In this work, complex and amide 2-phenyl-3,4-dihydroisoquinolin-1(2H)-one hydrogen bonds π-π interactions is characterized by diffraction. Such noncovalent in solution also exist, verified NMR, UV-Vis absorption, fluorescence emission measurements. Moreover, mixture B(C6F5)3·H2O, instead tested acids, shows tailing...
1. Salvianolic acid A (SalA) was found to attenuate plasma uric (UA) concentration and xanthine oxidase (XO) activity in acute myocardial infraction (AMI) rats, which characterized with developed mechanism-based pharmacokinetic–pharmacodynamic (PK-PD) model.2. AMI induced rats by coronary artery ligation. Surviving received a single intravenous dose of 5 mg/kg SalA normal saline. The concentrations were determined HPLC-MS/MS method. UA HPLC method XO measured spectrophotometrically. An...
Study Design: Retrospective study. Objective: To report the efficacy of anterior cervical decompression and fusion surgery as treatment method for cervicogenic headache (CeH). Summary Background Data: The exact diagnostic criteria optimal CeH is still under investigation. Methods: A total 34 consecutive patients (mean age 55.8 y) with (in addition to stenosis symptomatology) resistant nonoperative were treated by from 1 up 3 levels followed at least year. Clinical visual analog pain scale...
Background: Increasingly, different heterocyclic systems have been introduced into the steroid nucleus to significantly enhance antitumor activities of molecules. However, in this study, few literature precedents describing pyrazine heterocyclic-condensed modification an A-ring monomers were found, although group is thought be essential for potent anticancer activity clinically relevant drugs and natural dimers. Methods Results: Two series novel fused steroidal pyrazines designed efficiently...
Abstract Nowadays, mild hypothermia is widely used in the fields of post-cardiac arrest resuscitation, stroke, cerebral hemorrhage, large-scale infarction, and craniocerebral injury. In this paper, a locally mixed sub-low temperature device designed, cold hot water mixing experiment to simulate human blood transfer process. To set foundation for optimization heat system, static characteristics are analyzed by building mathematic model setting up experimental station. addition, affection...
Apoptosis plays pivotal role in the pathogenesis of degenerative disc diseases, which is primary contributor to low back pain. Although hydrogen sulfide (H2S) cell apoptosis well appreciated, effects and mechanism that H2S regulates program death intervertebral are not yet elucidated. In this study, we utilized nucleus pulposus (NP) from patients with lumbar herniation investigate relationship between endogenous NP cells human. Furthermore, analyzed rat study exogenous on hypoxia induced...