A5053664938- Retinal Diseases and Treatments
- Angiogenesis and VEGF in Cancer
- Glaucoma and retinal disorders
- Retinal Development and Disorders
- Cell Adhesion Molecules Research
- Retinopathy of Prematurity Studies
- Retinal Imaging and Analysis
- Cell death mechanisms and regulation
- Renal and related cancers
- Retinal and Optic Conditions
- Corneal Surgery and Treatments
- Advanced Glycation End Products research
- Neuroinflammation and Neurodegeneration Mechanisms
- Barrier Structure and Function Studies
- Connexins and lens biology
- Genetic and Kidney Cyst Diseases
- Ubiquitin and proteasome pathways
- Eicosanoids and Hypertension Pharmacology
- Platelet Disorders and Treatments
- Corneal surgery and disorders
- Ocular Oncology and Treatments
- Hippo pathway signaling and YAP/TAZ
- Erythrocyte Function and Pathophysiology
- Protease and Inhibitor Mechanisms
- DNA and Nucleic Acid Chemistry
University of Wisconsin–Madison
2016-2025
Smith-Kettlewell Eye Research Institute
2015-2024
Madison Group (United States)
2017
University of Wisconsin–Milwaukee
2015
McPherson College
2015
Second Affiliated Hospital of Zhejiang University
2015
Saint Louis University
2012
University of Puget Sound
2012
Geriatric Research Education and Clinical Center
2012
University of Washington
2012
DNA is the accepted target for cisplatin, but recent evidence has shed doubt on synthesis as critical process. L1210/0 cells incubated 2 hours with cisplatin progress to G phase of cell cycle and are arrested there several days. They then either in or die. In that eventually die, total transcription, polyadenylated [poly(A) + ] RNA synthesis, protein were markedly inhibited only after 48 hours. Nicotinamide adenine dinucleotide (NAD) adenosine triphosphate (ATP) levels decreased 3 Cell...
Oocyte loss, either directly through attrition (germ cell death) or indirectly follicular atresia (somatic granulosa death), is a fundamental event associated with defining the time of normal premature reproductive senescence in females. Although apoptosis has been reported to function as underlying mechanism responsible for death both germ cells and somatic ovary, final molecular steps which commit ovarian have not fully elucidated. To examine if repressor activity bcl-2 gene product...
The lack of capability to quantify oxygen metabolism noninvasively impedes both fundamental investigation and clinical diagnosis a wide spectrum diseases including all the major blinding such as age-related macular degeneration, diabetic retinopathy, glaucoma. Using visible light optical coherence tomography (vis-OCT), we demonstrated accurate robust measurement retinal metabolic rate (rMRO2) in rat eyes. We continuously monitored regulatory response consumption progressive hypoxic...
Vitamin D compounds inhibit the growth of a variety tumors in preclinical and clinical studies. Among mechanisms suggested for this inhibition is antiangiogenesis. Retinal angiogenesis basis vision loss several major blinding diseases. The purpose study was to evaluate antiangiogenic activity calcitriol (1,25-dihydroxyvitamin D(3)) vivo its effect on retinal endothelial cell (EC) proliferation, migration, capillary morphogenesis vitro.The mouse oxygen-induced ischemic retinopathy (OIR) model...
Histological examination of abdominal aortic aneurysm (AAA) tissues demonstrates extracellular matrix destruction and infiltration inflammatory cells. Previous work with mouse models AAA has shown that anti-inflammatory strategies can effectively attenuate formation. Thrombospondin-1 is a matricellular protein involved in the maintenance vascular structure homeostasis through regulation biological functions, such as cell proliferation, apoptosis, adhesion. Expression levels thrombospondin-1...
Abstract Thrombospondin‐1 (TSP1) is a natural inhibitor of angiogenesis. Its expression most prominent during the late stages vascular development and in adult vasculature. Our previous studies have shown that TSP1 promotes quiescent, differentiated phenotype endothelial cells. However, physiological role plays neovascularization requires further delineation. Here, we investigated retinal vasculature oxygen‐induced ischemic retinopathy. The density was increased TSP1‐deficient (TSP1‐/‐) mice...
Apoptosis of the developing metanephric kidney plays an important role in renal organogenesis. The bcl-2 is oncogene that inhibits apoptotic cell death a variety settings. (-/-) mice complete embryonic development but, contrast to (+/-) and (+/+) littermates, manifest growth retardation, hypopigmentation hair, lymphoid apoptosis, abnormal morphology, failure postnatally. To provide insight into mechanism for latter abnormalities, we examined kidneys from (-/-), (+/-), mice, as well day 12...
With the intention to modulate gene expression in vascular mural cells of remodeling vessels, we generated and characterized transgenic mouse lines with Cre recombinase under control platelet-derived growth factor receptor-β promoter, referred as Tg(Pdgfrb-Cre)(35Vli) . Transgenic mice were crossed Gt(ROSA)26Sor(tm1Sor) strain examined for activation by β-galactosidase activity, which was compared endogenous Pdgfrb expression. In addition, Pdgfrb-Cre used drive a conditional myc-tagged...
Mutation in CYP1B1 has been reported for patients with congenital glaucoma. However, the underlying mechanisms remain unknown. Here we show increased diurnal intraocular pressure (IOP) Cyp1b1-deficient (Cyp1b1(-/-)) mice. Cyp1b1(-/-) mice presented ultrastructural irregular collagen distribution their trabecular meshwork (TM) tissue along oxidative stress and decreased levels of periostin (Postn). Increased Postn were also detected human glaucomatous TM tissues. Furthermore, Postn-deficient...
Defects in the outer blood-retinal barrier have significant impact on pathogenesis of diabetic retinopathy and macular edema. However, detailed mechanisms involved remain largely unknown. This is, part, attributed to lack suitable animal cell culture models, including those mouse origin. We recently reported a method for retinal pigment epithelial (RPE) cells from wild-type transgenic mice. The RPE are responsible maintaining integrity whose dysfunction during diabetes has vision. Here we...
Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) is a member of the immunoglobulin superfamily molecules with important roles in angiogenesis and inflammation. However, molecular cellular mechanisms, role that specific PECAM-1 isoforms play these processes, remain elusive. We recently showed attenuation retinal vascular development neovascularization PECAM-1-deficient (PECAM-1−/−) mice. To gain further insight into we isolated primary cells (EC) from wild-type (PECAM-1+/+)...