A5073092010- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Interference and Gene Delivery
- Viral gastroenteritis research and epidemiology
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Metabolism and Genetic Disorders
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Cancer Research and Treatments
- Animal Virus Infections Studies
- Iron Metabolism and Disorders
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Cell death mechanisms and regulation
- Mitochondrial Function and Pathology
- T-cell and B-cell Immunology
- Hemoglobinopathies and Related Disorders
- Biotin and Related Studies
- Herpesvirus Infections and Treatments
- Advanced biosensing and bioanalysis techniques
- Heme Oxygenase-1 and Carbon Monoxide
- Neurobiology and Insect Physiology Research
Mayo Clinic in Arizona
2015-2025
WinnMed
2014-2024
Mayo Clinic
2015-2024
Mayo Clinic in Florida
2013-2024
Vector (United States)
2023-2024
Icahn School of Medicine at Mount Sinai
2018-2023
Institute of Molecular Medicine
2008-2023
Allen Institute for Brain Science
2021-2023
United States Marine Corps
2023
Westmead Hospital
2006-2019
DNA is the accepted target for cisplatin, but recent evidence has shed doubt on synthesis as critical process. L1210/0 cells incubated 2 hours with cisplatin progress to G phase of cell cycle and are arrested there several days. They then either in or die. In that eventually die, total transcription, polyadenylated [poly(A) + ] RNA synthesis, protein were markedly inhibited only after 48 hours. Nicotinamide adenine dinucleotide (NAD) adenosine triphosphate (ATP) levels decreased 3 Cell...
Cell death occurs by apoptosis during programmed deletion of cells and following exposure to cytotoxic agents. Central the mechanism is internucleosomal DNA digestion an endogenous endonuclease which thought mediate cell death. An axiom that involved a Ca2+/Mg2+-dependent endonuclease. During purification endonucleases from Chinese hamster ovary cells, we found little activity, but large amounts active below pH 7. This acidic was activated in intact reducing intracellular values 7 with...
Human species C adenovirus serotype 5 (Ad5) is the most common viral vector used in clinical studies worldwide. Ad5 vectors infect liver cells vivo with high efficiency via a poorly defined mechanism, which involves virus binding to vitamin K-dependent blood coagulation factors. Here, we report that major capsid protein, hexon, binds human factor X (FX) an affinity of 229 pM. This 40-fold stronger than reported fiber for cellular receptor coxsackievirus and receptor, CAR. Cryoelectron...
A prospective trial of continuous chelation therapy in children with homozygous thalassaemia on a high transfusion regimen was started April 1966. The effect treatment iron concentration the liver and hepatic histology examined 49 biopsy specimens obtained from nine chelator-treated patients control between 1966 1973. Chelation associated significant reduction concentration, by end values for two groups showed no overlap. similar difference seen amount fibrosis, which little or change but...
Poly(ethylenimine) (PEI) is a synthetic polycation that has been used successfully for gene delivery both in vitro and vivo due, theory, to form of protection afforded the carried plasmids. In this study stability PEI/DNA complexes was demonstrated using deoxyribonuclease (DNase) 1 DNase 2, various levels pH, increasing exposure times. DNA complexed with PEI not degraded when exposed at least 25 Units either enzyme 24 h while uncomplexed forms same plasmid were digested 0.010 0.05 or 0.003 2...
Adenoviruses are robust gene delivery vectors in vivo, but limited by their propensity to provoke strong innate and adaptive responses. Previous work has demonstrated that polyethylene glycol (PEG) modification of adenovirus can protect the from preexisting immune responses reducing protein–protein interactions. To test whether PEGylation reduce interactions with cells, first-generation (FG-Ad) helper-dependent (HD-Ad) Ad5 were PEGylated SPA-PEG tested vitro vivo. We demonstrate increasing...
Targeted fluorescent molecular imaging probes may provide an optimal means of detecting disease. Stable, organic fluorophores can be repeatedly excited in vivo by propagated light and consequentially large signal-to-noise ratios (SNRs) for image detection target tissues. In the literature, many small animal studies are performed with a red excitable dye, Cy5.5, conjugated to targeting component. We report comparison performance near-IR (NIR) red-excitable dye. Epidermal growth factor (EGF)...
An axiom of apoptosis is that increases in cytosolic Ca2+ activate a Ca2+Mg2+-dependent endonuclease. However, when HL-60 human promyelocytic leukemia cells were incubated with the ionophore ionomycin varied extracellular Ca2+, DNA digestion was independent Ca2+. Under these conditions, intracellular concentrations did not correlate observed digestion. In contrast, acidification correlated well These data indicate increased primary signal for endonuclease activation all forms apoptosis, but...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInteraction of trans-diamminedichloroplatinum(II) with DNA: formation monofunctional adducts and their reaction glutathioneAlan Eastman Michael A. BarryCite this: Biochemistry 1987, 26, 12, 3303–3307Publication Date (Print):June 16, 1987Publication History Published online1 May 2002Published inissue 16 June 1987https://pubs.acs.org/doi/10.1021/bi00386a009https://doi.org/10.1021/bi00386a009research-articleACS PublicationsRequest reuse...
Background: Type 1 long QT syndrome (LQT1) is caused by loss-of-function variants in the KCNQ1 -encoded K v 7.1 potassium channel α-subunit that essential for cardiac repolarization, providing slow delayed rectifier current. No current therapies target molecular cause of LQT1. Methods: A dual-component suppression-and-replacement (SupRep) gene therapy was created cloning a short hairpin RNA and RNA-immune cDNA modified with synonymous site, into single construct. The ability KCNQ1-SupRep to...
DNA degradation is a fundamental problem for any gene therapy or genetic immunization approach, since destruction of incoming genes translates into loss expression. To characterize the biology after naked injection, location and levels tissue nucleases were assessed. Extracts from serum, kidney, liver mice had high calcium-dependent endonuclease activity. High acidic activity identified in spleen, liver, skin with little skeletal cardiac muscle. Relatively exonuclease was observed tissue....