A5079708858- Immunotherapy and Immune Responses
- Osteoarthritis Treatment and Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Inflammatory mediators and NSAID effects
- Immune cells in cancer
- RNA regulation and disease
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Cytokine Signaling Pathways and Interactions
- Dermatology and Skin Diseases
- Skin Protection and Aging
- interferon and immune responses
- Bone and Dental Protein Studies
- Advancements in Transdermal Drug Delivery
- Chemokine receptors and signaling
- Neuroscience and Neural Engineering
- Connective Tissue Growth Factor Research
- Respiratory viral infections research
- Bone and Joint Diseases
- Viral gastroenteritis research and epidemiology
- Neurological disorders and treatments
- Peripheral Neuropathies and Disorders
- Poxvirus research and outbreaks
- Bone Metabolism and Diseases
University of Toronto
2013-2023
Schroeder Arthritis Institute
2021
Krembil Research Institute
2020
University Health Network
2013-2020
University of Hong Kong
2019
Massachusetts General Hospital
2017
First Hospital of Jilin University
2013
Jilin University
2013
University of Portsmouth
2013
Washington University in St. Louis
2012-2013
Systemic administration of IL-12p70 has demonstrated clinical activity in cancer patients, but dose-limiting toxicities have hindered its incorporation vaccine formulations. Here, we report on the immunological and outcomes upon vaccination with CD40L/IFN-γ-matured, IL-12p70-producing DCs.7 HLA-A*0201+ newly diagnosed stage IV melanoma patients were immunized against gp100 antigen using autologous peptide-pulsed, CD40L/IFN-γ-matured DCs. PBMCs taken weekly for immune monitoring by tetramer...
Despite causing regular seasonal epidemics with substantial morbidity, mortality and socioeconomic burden, there is still a lack of research into influenza B viruses (IBVs). In this study, we provide for the first time systematic investigation on tropism, replication kinetics pathogenesis IBVs in human respiratory tract. Physiologically relevant ex vivo explant cultures bronchus lung, airway organoids, vitro differentiated primary bronchial epithelial cells type-I-like alveolar were used to...
Viral myocarditis follows a fatal course in ≈30% of patients. Interleukin-1 receptor-associated kinase 4 (IRAK4), major nodal signal transducer innate immunity, can play pivotal role host inflammatory response. We sought to determine how IRAK4 modulates inflammation and outcome mouse model viral myocarditis.Myocarditis was induced after intraperitoneal inoculation coxsackievirus B3 into C57Bl/6 IRAK4-deficient mice their littermate controls. Mortality proliferation were markedly reduced...
<h3>BACKGROUND AND PURPOSE:</h3> Autoimmune encephalitis is a rare condition in which autoantibodies attack neuronal tissue, causing neuropsychiatric disturbances. This study sought to evaluate MR imaging findings associated with subtypes and categories of autoimmune encephalitis. <h3>MATERIALS METHODS:</h3> Cases specific were identified from the medical record (2009–2019). excluded if no brain was available, antibodies demyelinating disease, or >1 concurrent antibody present....
Abstract Brief exposure of skin to near-infrared (NIR) laser light has been shown augment the immune response intradermal vaccination and thus act as an immunologic adjuvant. Although evidence indicates that NIR adjuvant capacity activate innate subsets including dendritic cells (DCs) in conventional adjuvants do, precise immunological mechanism by which acts is largely unknown. In this study we sought identify cellular target using established mouse model influenza examining alteration...
Abstract Upon Ag exposure, most memory T cells undergo restimulation-induced cell death. In this article, we describe a novel synthetic agonist, an N-terminal extended decamer peptide expressed as single-chain trimer, the amino-terminal MHC class I trimer (AT-SCT), which preferentially promotes growth of human CD8+ with minimal Using CMV pp65 and melanoma gp100 Ags, observe in vitro numerical expansion clonally diverse polyfunctional population Ag-specific from healthy individuals vaccinated...
2525 Background: CD40L/IFN-γ matured Dendritic Cells (DCs) produce IL-12 and are potent antigen-presenting cells for naïve resting T cells. We sought to determine the magnitude kinetics of CD8+ cell growth in patients receiving autologous DC identify biomarkers associated with clinical outcome. Methods: A phase I trial (NCT00683670) incorporating ex vivo maturation DCs pulsed three well characterized gp100 melanoma antigen derived peptides (G154, G209-2M, G280-9V) was initiated enrollment...
Abstract Systemic administration of IL-12p70 has demonstrated clinical activity in cancer patients but dose-limiting toxicities have hindered its incorporation vaccine formulations. In this proof-of-concept phase I trial (NCT00683670), we report on the immunological and outcomes upon vaccination newly diagnosed stage IV melanoma with CD40L/IFN-γ matured producing dendritic cells (mDC). HLA-A*0201+ individuals treatment naïve metastatic were vaccinated mDC pulsed gp100-derived peptides,...
Abstract Ex-vivo generated dendritic cells (DC) have been used as therapeutic vaccines for the treatment of patients with metastatic cancer. However, these first-generation consist DC activated to up-regulate costimulatory molecules minimal absent IL-12p70 production. Inflammatory cytokines, primarily and interferons, provide a critical third signal along antigen (signal 1) costimulation 2) optimal effector CD8 T cell expansion/differentiation memory formation. In order generate...
Abstract Metastasis is a major cause of death in cancer patients. We report here the use multiplex proteomic analysis approach to vitro and vivo identify biomarkers involved melanoma invasion/metastasis. An quantitative profile secretome (&gt;200 proteins) was done on non-metastatic cell lines (A375, LH, M14, DM.6, Lox, WUmel19) their metastatic RhoC-expressing counterparts (A375-RhoC, LH-RhoC, M14-RhoC, DM.6-RhoC, Lox-RhoC, WUmel19-RhoC). A heat map cluster performed characterize...