A5039744313- Nanoplatforms for cancer theranostics
- Immunotherapy and Immune Responses
- Photodynamic Therapy Research Studies
- Laser Applications in Dentistry and Medicine
- Chemokine receptors and signaling
- Medical and Biological Ozone Research
- Cancer Mechanisms and Therapy
- Heme Oxygenase-1 and Carbon Monoxide
- Nanoparticle-Based Drug Delivery
- Skin Protection and Aging
- Angiogenesis and VEGF in Cancer
- Nitric Oxide and Endothelin Effects
- Advancements in Transdermal Drug Delivery
- Cancer, Hypoxia, and Metabolism
- Cardiovascular Health and Disease Prevention
- Cardiac tumors and thrombi
- Ocular and Laser Science Research
- Cancer Cells and Metastasis
- Ocular Infections and Treatments
- Cancer Research and Treatments
- Ovarian cancer diagnosis and treatment
- Lymphatic System and Diseases
- Cerebrovascular and Carotid Artery Diseases
- Cytokine Signaling Pathways and Interactions
- Infrared Thermography in Medicine
Massachusetts General Hospital
2016-2025
Gordon Center for Medical Imaging
2018-2025
Harvard University
2013-2025
VA Boston Healthcare System
2024
MGH Institute of Health Professions
2023
Hokkaido University
2014-2022
C4 Imaging (United States)
2020
Charles River Associates
2014
Office of Infectious Diseases
2014
Yale University
2004-2013
Heme oxygenase is a heme-oxidizing enzyme which gener- ates biliverdin and carbon monoxide (CO).The present study was designed to elucidate whether CO endogenously produced by this serves as an active vasorelaxant in the hepatic microcirculation.Microvasculature of iso- lated perfused rat liver visualized dual-color digital microfluorography alternately monitor sinusoidal lining fat-storing Ito cells.In control liver, flux venous effluent ranged at 0.7 nmol/min per gram liver.Administration...
Passive targeting of large nanoparticles by the enhanced permeability and retention (EPR) effect is a crucial concept for solid tumor in cancer nanomedicine. There is, however, trade-off between long-term blood circulation their nonspecific background tissue uptake. To define this size-dependent EPR effect, near-infrared fluorophore-conjugated polyethylene glycols (PEG-ZW800s; 1-60 kDa) are designed biodistribution, pharmacokinetics, renal clearance evaluated tumor-bearing mice. The...
The chemokine CXCL12 and its receptor CXCR4 are expressed widely in human cancers, including ovarian cancer, which they associated with disease progression at the levels of tumor cell proliferation, invasion, angiogenesis. Here, we used an immunocompetent mouse model intraperitoneal papillary epithelial cancer to show that modulation CXCL12/CXCR4 axis has multimodal effects on pathogenesis induction antitumor immunity. siRNA-mediated knockdown BR5-1 cells constitutively express reduced...
NO has been shown to mediate angiogenesis; however, its role in vessel morphogenesis and maturation is not known. Using intravital microscopy, histological analysis, α–smooth muscle actin chondroitin sulfate proteoglycan 4 staining, microsensor measurements, an synthase (NOS) inhibitor, we found that mediates mural cell coverage as well branching longitudinal extension but the circumferential growth of blood vessels B16 murine melanomas. NO-sensitive fluorescent probe 4,5-diaminofluorescein...
Lymphatic metastasis is a critical determinant of cancer prognosis. Recently, several lymphangiogenic molecules such as vascular endothelial growth factor (VEGF)-C and VEGF-D were identified. However, the mechanistic understanding lymphatic still in infancy. Nitric oxide (NO) plays crucial role regulating blood vessel function well function. NO synthase (NOS) expression correlates with metastasis. causal relationship between NOS has not been documented. To this end, we first show that both...
Islet transplantation represents a potentially curative approach for individuals with Type I Diabetes. The requirement systemic immune suppression to control immune-mediated rejection of transplanted islets and the limited human islet supply represent significant roadblocks progress this approach. microencapsulation in alginate offers protection absence immunosuppression, but does not support long-term survival. chemokine, CXCL12, can repel effector T cells while recruiting...
The strategy of structure-inherent tumor targeting (SITT) with cyanine-based fluorophores is receiving more attention because no chemical conjugation moieties required. However, the mechanism behind SITT has not yet been well explained. Here, it demonstrated that heptamethine-cyanine-based possess only targetability microenvironments without need for additional ligands but also second near-infrared spectral window (NIR-II) imaging capabilities, i.e., minimum scattering and ultralow...
Akt mediates postnatal angiogenesis through eNOS signaling.
The current management of patients with stroke intravenous thrombolysis and endovascular thrombectomy is effective only when it timely performed on an appropriately selected but minor fraction patients. development novel adjunctive therapy highly desired to reduce morbidity mortality stroke. Since endothelial dysfunction implicated in the pathogenesis featured suppressed nitric oxide synthase (eNOS) concomitant deficiency, restoring represents a promising approach treating injury.
Functional interactions between the initial and collecting lymphatics, as well molecular players involved, remain elusive. In this study, we assessed influence of nitric oxide (NO) on lymphatic fluid velocity flow, using a mouse tail model that permits intravital microscopy microlymphangiography. We found NO synthase (NOS) inhibition decreased in without any effect their morphology. Using same model, similar eNOS-/- mice treated with selective endothelial NOS (eNOS) inhibitor. Next,...
Abstract The role of placenta growth factor (PlGF) in pathologic angiogenesis is controversial. effects PlGF on growth, angiogenesis, and metastasis from orthotopic tumors are not known. To this end, we stably transfected three human cancer cell lines (A549 lung, HCT116 colon, U87-MG glioblastoma) with plgf-2 full-length cDNA. Overexpression did affect tumor proliferation or migration vitro. PlGF-overexpressing grown orthotopically ectopically was impaired all models. This decrease...
Phosphorylation of eNOS, an important post-translational modulator its enzymatic activity, is reduced in diabetes mellitus. We hypothesized that modulation eNOS phosphorylation could overcome diabetic vascular dysfunction and improves the outcome to stroke.We used db/db mouse model type 2 mated mice with knock-in carry single amino acid mutations at S1176 site; phosphomimetic SD mutation (serine replaced by aspartate) shows increased whereas unphosphorylatable SA alanine) decreased activity....
Hyperlipidemia is a highly prevalent risk factor for coronary and cervical atherosclerosis stroke. However, even in the absence of overt atherosclerosis, hyperlipidemia disrupts endothelial smooth muscle function. We investigated impact on resting-brain perfusion, fundamental cerebrovascular reflexes, dynamic perfusion defect during acute focal ischemia hyperlipidemic apolipoprotein E knockout mice before development flow-limiting atherosclerotic stenoses. Despite elevated blood pressures,...
Among modalities of cancer immunotherapy, near-infrared photoimmunotherapy (NIR-PIT) has reached significant preclinical and clinical stages quickly evolved over the last 5 years. NIR-PIT uses deep-penetrable NIR light to induce physicochemical changes in antibody-photosensitizer conjugate (APC), leading resultant necrosis immunogenic cell death (ICD) cells. Alternatively, other types photomedicine use photosensitizers convert absorbed energy either into reactive oxygen species for...
The residual tumor after surgery is the most significant prognostic factor of patients with epithelial ovarian cancer. Near-infrared (NIR) fluorescence-guided actively utilized for localization and complete resection during surgery. However, currently available contrast-enhancing agents display low on-target binding, unfavorable pharmacokinetics, toxicity, thus not ideal clinical use. Here we report ultrabright stable squaraine fluorophores optimal pharmacokinetics by introducing an...
This study aimed to examine topographic distribution of microvascular NO generation in vivo. To this end, nitrosonium ion (NO + )–sensitive diaminofluorescein diacetate was superfused continuously on the rat mesentery and fluorescence visualized microvessels through laser confocal microfluorography. Two major sites exhibited a time-dependent elevation fluorescence: endothelia mast cells. As judged by sensitivity local application different inhibitors synthase (NOS), availability arteriolar...
Abstract In this study, we evaluated the role of tumor cell and stroma sensitivity as determinants radiation-induced growth delay. A DNA double-strand break repair–defective DNA-PKcs−/− line its radioresistant DNA-PKcs+/+–transfected counterpart were used to initiate tumors in nude hypersensitive severe combined immunodeficient (SCID) mice. Insertion human DNA-PKcs+/+ gene substantially increased intrinsic radioresistance cells decreased response radiation both SCID Tumor radiosensitivity...