A5004364143- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Erythrocyte Function and Pathophysiology
- Hippo pathway signaling and YAP/TAZ
- Hedgehog Signaling Pathway Studies
- Cancer Genomics and Diagnostics
- Digestive system and related health
- Cancer Research and Treatments
- Ubiquitin and proteasome pathways
- Wnt/β-catenin signaling in development and cancer
- Acute Myeloid Leukemia Research
- Cellular Mechanics and Interactions
- Peptidase Inhibition and Analysis
- Genetic factors in colorectal cancer
FPInnovations
2024
Hospital for Sick Children
2017-2024
University of Toronto
2017-2024
Princess Margaret Cancer Centre
2017
University Health Network
2017
Abstract Stomach and intestinal stem cells are located in discrete niches called the isthmus crypt, respectively. Recent studies have demonstrated a surprisingly conserved role for Wnt signaling gastrointestinal development. Although stromal secrete ligands to promote cell renewal, source of stomach is still unclear. Here, by performing single analysis, we identify populations with transcriptome signatures that between intestine. In close proximity epithelial cells, these perictye-like...
Stem cells perceive and respond to biochemical physical signals maintain homeostasis. Yet, it remains unclear how stem sense mechanical from their niche in vivo. In this work, we investigated the roles of PIEZO mechanosensitive channels intestinal cell (ISC) niche. We used mouse genetics single-cell RNA sequencing analysis assess requirement for ISC maintenance. vivo measurement basement membrane stiffness showed that ISCs reside a more rigid microenvironment at bottom crypt....
Peptic ulcer disease caused by environmental factors increases the risk of developing gastric cancer (GC), one most common and deadly cancers in world. However, mechanisms underlying this association remain unclear. A major type GC uniquely undergoes spasmolytic polypeptide-expressing metaplasia (SPEM) followed intestinal metaplasia. Notably, intestinal-type patients with high levels YAP signaling exhibit a lower survival rate poor prognosis. overexpression cells induces atrophy, metaplasia,...
ABSTRACT Stem cells continuously perceive and respond to various environmental signals maintain homeostasis. In addition biochemical factors, the stem cell niche is subjected mechanical physical cues. However, it remains unclear how can sense from their in vivo . Since intestinal constantly directly face external environment, we investigated roles of mechano-sensing PIEZO ion channels gut niche. By employing mouse genetics performing single-cell RNAseq analysis, revealed absolute requirement...
Cell surface proteins offer significant cancer therapeutic potential attributable to their accessible membrane localization and central role in cellular signaling. Despite this, promise remains largely untapped due the technical challenges inherent profiling cell proteins. Here, we employed N-glycoproteomics analyze 85 patient-derived xenografts (PDX), constructing Glyco PDXplorer - an vivo pan-cancer atlas of cancer-derived We developed a target discovery pipeline prioritize with favorable...