A5035637290- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Cell Image Analysis Techniques
- Microtubule and mitosis dynamics
- Cell Adhesion Molecules Research
- Clinical Nutrition and Gastroenterology
- Erythrocyte Function and Pathophysiology
- Inflammatory mediators and NSAID effects
- Neuroscience and Neural Engineering
- Protease and Inhibitor Mechanisms
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Hippo pathway signaling and YAP/TAZ
- Nanoparticle-Based Drug Delivery
- Electrospun Nanofibers in Biomedical Applications
- Hedgehog Signaling Pathway Studies
- Bone Tissue Engineering Materials
- Retinal Development and Disorders
- Microfluidic and Bio-sensing Technologies
- Pancreatic and Hepatic Oncology Research
- Photoacoustic and Ultrasonic Imaging
- Graphene and Nanomaterials Applications
- Sirtuins and Resveratrol in Medicine
- Angiogenesis and VEGF in Cancer
Institut Curie
2021-2024
Université Paris Sciences et Lettres
2023-2024
Centre National de la Recherche Scientifique
2023-2024
Temple University
2017-2022
Northwestern University
2015
Stem cells perceive and respond to biochemical physical signals maintain homeostasis. Yet, it remains unclear how stem sense mechanical from their niche in vivo. In this work, we investigated the roles of PIEZO mechanosensitive channels intestinal cell (ISC) niche. We used mouse genetics single-cell RNA sequencing analysis assess requirement for ISC maintenance. vivo measurement basement membrane stiffness showed that ISCs reside a more rigid microenvironment at bottom crypt....
Abstract Invasive and non-invasive cancer cells can invade together during cooperative invasion. However, the events leading to it, role of epithelial-mesenchymal transition consequences this may have on metastasis are unknown. In study, we demonstrate that isogenic 4T1 67NR breast sort from each other in 3D spheroids, followed by By time-lapse microscopy, show invasive move more persistently compared 67NR, sorting accumulating at spheroid-matrix interface, a process dependent cell-matrix...
ABSTRACT The process of tumor cell invasion and metastasis includes assembly invadopodia, protrusions capable degrading the extracellular matrix (ECM). effect cycle progression on invadopodia has not been elucidated. In this study, by using fluorescent markers, we show in 2D 3D cultures, as well vivo, that breast carcinoma cells assemble invade into surrounding ECM preferentially during G1 phase. expression (MT1-MMP, also known MMP14, cortactin) localization (Tks5; SH3PXD2A) components are...
Endothelial cells (ECs) that are differentiated from induced pluripotent stem (iPSCs) can be used in establishing disease models for personalized drug discovery or developing patient-specific vascularized tissues organoids. However, a number of technical challenges often associated with iPSC-ECs culture, including instability the endothelial phenotype and limited cell proliferative capacity over time. Early senescence is believed to primary mechanism underlying these limitations. Sirtuin1...
The invasion of cancer cells from the primary tumor into adjacent healthy tissues is an early step in metastasis. Invasive pose a major clinical challenge because no efficient method exist for their elimination once dissemination underway. A better understanding mechanisms regulating cell may lead to development novel potent therapies. Due physiological resemblance tumors, spheroids embedded collagen I have been extensively utilized by researchers study governing extracellular matrix (ECM)....
ABSTRACT Stem cells continuously perceive and respond to various environmental signals maintain homeostasis. In addition biochemical factors, the stem cell niche is subjected mechanical physical cues. However, it remains unclear how can sense from their in vivo . Since intestinal constantly directly face external environment, we investigated roles of mechano-sensing PIEZO ion channels gut niche. By employing mouse genetics performing single-cell RNAseq analysis, revealed absolute requirement...
The invasion of cancer cells from the primary tumor into adjacent healthy tissues is an early step in metastasis. Invasive pose a major clinical challenge because no efficient method exist for their elimination once dissemination underway. A better understanding mechanisms regulating cell may lead to development novel potent therapies. Due physiological resemblance tumors, spheroids embedded collagen I have been extensively utilized by researchers study governing extracellular matrix (ECM)....
Abstract Invasive and non-invasive cancer cells can invade together during the cooperative invasion. However, events leading to it, role of EMT consequences this may have on metastasis are unknown. In study, we demonstrate that isogenic 4T1 67NR breast sort from each other in 3D spheroids, followed by By time-lapse microscopy, show invasive move more persistently compared 67NR, sorting accumulating at spheroid-ECM interface, a process dependent cell-ECM adhesions independent E-cadherin...
Abstract Invadopodia are cancer cell protrusions rich in structural proteins (e.g. Tks5, cortactin) and proteases MT1-MMP) responsible for degradation of the extracellular matrix (ECM). Tumor invasion metastasis require cells to be both proliferative invasive, i.e. migrate through tissue assemble invadopodia. While several studies addressed how motility parameters change throughout cycle, relationship between invadopodia cycle progression has not been elucidated. In this study, using...
Abstract Multiple clones of cancer cells can seed metastases via collective invasion and dissemination. While it is known that cooperate during invasion, the events leading to effects may have on metastasis are not known. In this study, we demonstrate that, when mixed in 3D spheroids, isogenic 4T1 67NR breast sort from each other, followed by their cooperative invasion. By time-lapse microscopy heterogenous spheroids embedded collagen I, show invasive reach spheroid edge remain there, while...