A5034966136- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Flexible and Reconfigurable Manufacturing Systems
- Force Microscopy Techniques and Applications
- Cell Adhesion Molecules Research
- Cancer Cells and Metastasis
- Manufacturing Process and Optimization
- Collagen: Extraction and Characterization
- Erythrocyte Function and Pathophysiology
- Microfluidic and Bio-sensing Technologies
- Near-Field Optical Microscopy
- Microtubule and mitosis dynamics
- Digital Transformation in Industry
- Photoacoustic and Ultrasonic Imaging
- Physics and Engineering Research Articles
- Neurofibromatosis and Schwannoma Cases
- Hippo pathway signaling and YAP/TAZ
- Molecular Sensors and Ion Detection
- Neuroscience and Neural Engineering
- Wnt/β-catenin signaling in development and cancer
- Bone Tissue Engineering Materials
- Cellular transport and secretion
- RNA and protein synthesis mechanisms
- S100 Proteins and Annexins
- Pluripotent Stem Cells Research
European Molecular Biology Laboratory
2016-2025
European Molecular Biology Organization
2022
European Molecular Biology Laboratory
2019-2020
ETH Zurich
2014-2017
University College London
2015-2016
MRC Laboratory for Molecular Cell Biology
2015-2016
International Institute of Molecular and Cell Biology
2010-2016
Max Planck Institute of Molecular Cell Biology and Genetics
2010-2016
Medical Research Council
2016
University of Bern
2014
Protrusion formation is an essential step during cell migration. Cells migrating in three-dimensional environments and vivo can form a wide variety of protrusion types, including actin polymerization-driven lamellipodia, contractility-driven blebs. The ability to switch between different protrusions has been proposed facilitate motility complex promote cancer dissemination. However, plasticity so far mostly investigated the context transitions amoeboid mesenchymal migration modes, which...
Cell shape and motility are primarily controlled by cellular mechanics. The attachment of the plasma membrane to underlying actomyosin cortex has been proposed be important for processes involving deformation. However, little is known about actual function membrane-to-cortex (MCA) in cell protrusion formation migration, particular context developing embryo. Here, we use a multidisciplinary approach study MCA zebrafish mesoderm endoderm (mesendoderm) germ layer progenitor cells, which migrate...
Abstract The mechanical wiring between cells and their surroundings is fundamental to the regulation of complex biological processes during tissue development, repair or pathology. Traction force microscopy (TFM) enables determination actuating forces. Despite progress, important limitations with intrusion effects in low resolution 2D pillar-based methods disruptive intermediate steps cell removal substrate relaxation high-resolution continuum TFM need be overcome. Here we introduce a novel...
Cell differentiation typically occurs with concomitant shape transitions to enable specialized functions. To adopt a different shape, cells need change the mechanical properties of their surface. However, whether cell surface mechanics control process has been relatively unexplored. Here we show that membrane gate exit from naive pluripotency mouse embryonic stem cells. By measuring tension during early differentiation, find release plasma underlying actin cortex when transitioning primed...
Abstract Aminoacyl-tRNA synthetases (aaRSs) constitute a family of ubiquitously expressed essential enzymes that ligate amino acids to their cognate tRNAs for protein synthesis. Recently, aaRS mutations have been linked various human diseases; however, how these lead diseases has remained unclear. In order address the importance aminoacylation fidelity in multicellular organisms, we generated an amino-acid double-sieving model Drosophila melanogaster using phenylalanyl-tRNA synthetase...
High directional persistence is often assumed to enhance the efficiency of chemotactic migration. Yet, cells in vivo usually display meandering trajectories with relatively low persistence, and control function during cell migration three-dimensional environments are poorly understood. Here, we use mesendoderm progenitors migrating zebrafish gastrulation as a model system investigate vivo. We show that progenitor alternate persistent run phases tumble result reorientation. Runs characterized...
Stem cells perceive and respond to biochemical physical signals maintain homeostasis. Yet, it remains unclear how stem sense mechanical from their niche in vivo. In this work, we investigated the roles of PIEZO mechanosensitive channels intestinal cell (ISC) niche. We used mouse genetics single-cell RNA sequencing analysis assess requirement for ISC maintenance. vivo measurement basement membrane stiffness showed that ISCs reside a more rigid microenvironment at bottom crypt....
Abstract In multicellular systems, the migration pattern of individual cells critically relies on interactions with neighboring cells. Depending strength these interactions, either move as a collective, observed during morphogenesis and wound healing, or migrate individually, it is case for immune fibroblasts. Mediators cell-cell adhesions, such cadherins coordinate collective dynamics by linking cytoskeleton However, whether intercellular binding alone triggers signals that originate from...
Metastatic progression of tumors requires the coordinated dissemination cancerous cells through interstitial tissues and their replication in distant body locations. Despite importance cancer treatment decisions, key factors, such as cell shape adaptation role it plays dense tissue invasion by cells, are not well understood. Here, we employ a 3D electrohydrodynamic nanoprinting technology to generate vertical arrays topographical pores that mimic resistance mesenchymal migration order...
Schnellere Produktionsanlaufe, Qualitatsverbesserungen von Hard- und Software sowie Produktivitatssteigerungen durch hohere Anlagenverfugbarkeiten schnellere, robustere Integrationsprozesse - dies sind die grosen Potentiale, Automobilunternehmen den Ein-satz der Virtuellen Inbetriebnahme erschliesen wollen. Wenngleich in vergangenen Jahren grose Fortschritte im Bereich erzielt wurden, gibt es immer noch einige Aspekte, einem flachendeckenden Produktiveinsatz Automobilindustrie...
Cell-cell fusion is an evolutionarily conserved process that essential for many functions, including fertilisation and the formation of placenta, muscle osteoclasts, multinucleated cells are unique in their ability to resorb bone. The mechanisms osteoclast multinucleation involve dynamic interactions between actin cytoskeleton plasma membrane still poorly characterized. Here, we found moesin, a cytoskeletal linker protein member Ezrin/Radixin/Moesin (ERM) family, activated during maturation...
Abstract The cellular actin cortex provides crucial mechanical support and plays critical roles in numerous functions, including cell division migration. proteins of the ERM family, ezrin, radixin, moesin, are central to these processes by linking plasma membrane cytoskeleton. To investigate individual contributions three leukocyte migration, we generated single triple knock-out macrophages. Surprisingly, found that even absence ERMs, macrophages can still form different structures promoting...
Abstract Cell differentiation typically occurs with concomitant shape transitions to enable specialized functions. To adopt a different shape, cells need change the mechanical properties of their surface. However, whether conversely cell surface mechanics control process has been relatively unexplored. Here, we show that membrane gate exit from naïve pluripotency mouse embryonic stem cells. By measuring tension during differentiation, find release plasma underlying actin cortex when...
In order to meet the various challenges in automotive industry, new and integrated methodologies for production planning ramp-up processes are required. Thus, a concept automated PLC software generation based on standardized digital process information is introduced with special focus needs of body shop industry. The foundation plan that describes steps graphical way. For elements attributes introduced. So contains stepwise specialization all necessary program. This paper additionally...
Speziell in der Automobilindustrie werden seit mehreren Jahren digitale Simulationsmethoden und -technologien zur Absicherung von Entwicklungs- Planungsergebnissen erfolgreich eingesetzt. Als letzter Schritt Absicherungskette wird dabei die virtuelle Inbetriebnahme als Ubergang zwischen digitaler realer Fabrik angesehen. D. h., reale Steuerungsprogramme im Zusammenspiel mit digitalen Modellen geplanten Fertigungssysteme validiert optimiert. Zur effizienten fehlerfreien Erstellung...
Abstract In multicellular systems, the migration pattern of individual cells critically relies on interactions with neighboring cells. Depending strength these interactions, either move as a collective, observed during morphogenesis and wound healing, or migrate individually, it is case for immune fibroblasts. Transducers cell-cell adhesions, such cadherins coordinate collective dynamics by linking cytoskeleton However, whether intercellular binding alone triggers signals that originate from...