A5067823651- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Bone health and treatments
- Force Microscopy Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Microtubule and mitosis dynamics
- Bone Tissue Engineering Materials
- Hippo pathway signaling and YAP/TAZ
- Cell Image Analysis Techniques
- RNA Research and Splicing
- Collagen: Extraction and Characterization
- Caveolin-1 and cellular processes
- Tissue Engineering and Regenerative Medicine
- Polymer Surface Interaction Studies
- Silk-based biomaterials and applications
- Orthopaedic implants and arthroplasty
- Adipose Tissue and Metabolism
- Neurogenesis and neuroplasticity mechanisms
- Mesenchymal stem cell research
- Nuclear Structure and Function
- Adipokines, Inflammation, and Metabolic Diseases
- Microfluidic and Capillary Electrophoresis Applications
- Nerve injury and regeneration
TU Dresden
2016-2025
Center for Systems Biology Dresden
2019-2025
University Hospital Carl Gustav Carus
2024
Leibniz Institute of Polymer Research
2023
Biotechnology Research Center
2018-2020
Queensland University of Technology
2012-2014
National Center for Genetic Engineering and Biotechnology
2007-2014
Princess Alexandra Hospital
2012-2013
Prostate Cancer Foundation of Australia
2013
Cells can enter into a dormant state when faced with unfavorable conditions. However, how cells and recover from this is still poorly understood. Here, we study dormancy in different eukaryotic organisms find it to be associated significant decrease the mobility of organelles foreign tracer particles. We show that reduced caused by an influx protons marked acidification cytoplasm, which leads widespread macromolecular assembly proteins triggers transition cytoplasm solid-like increased...
Flexible tubular structures fabricated from solution electrospun fibers are finding increasing use in tissue engineering applications. However it is difficult to control the deposition of due chaotic nature electrospinning jet. By using non-conductive polymer melts instead solutions path and collection fiber becomes predictable. In this work we demonstrate melt polycaprolactone a direct writing mode onto rotating cylinder. This allows design fabrication tubes 20 μm diameter with controllable...
Cell stiffness is a sensitive indicator of physiological and pathological changes in cells, with many potential applications biology medicine. A new method, real-time deformability cytometry, probes cell at high throughput by exposing cells to shear flow microfluidic channel, allowing for mechanical phenotyping based on single-cell deformability. However, observed deformations the channel not only are determined stiffness, but also depend size relative size. Here, we disentangle mutual...
We have characterized early steps of α 2 β 1 integrin-mediated cell adhesion to a collagen type I matrix by using single-cell force spectroscopy. In agreement with the role as receptor, -expressing Chinese hamster ovary (CHO)-A2 cells spread rapidly on matrix, whereas -negative CHO wild-type adhered poorly. Probing CHO-A2 detachment forces over contact time range 600 s revealed nonlinear response. During first 60 s, increased slowly, and associated smallest rupture events were consistent...
During vertebrate gastrulation, progenitor cells of different germ layers acquire specific adhesive properties that contribute to layer formation and separation. Wnt signals have been suggested function in this process by modulating the levels adhesion between layers, however, direct evidence for is still lacking. Here we show Wnt11, a key signal regulating gastrulation movements, needed zebrafish mesendodermal fibronectin, an abundant extracellular matrix component during gastrulation. To...
Abstract Studies of the role actin in tumour progression have highlighted its key contribution cell softening associated with invasion. Here, using a human breast line conditional Src induction, we demonstrate that cells undergo stiffening state prior to acquiring malignant features. This is characterized by transient accumulation stress fibres and upregulation Ena/VASP-like (EVL). EVL, turn, organizes leading stiffening, ERK-dependent proliferation, as well enhancement activation towards...
ABSTRACT The skeleton is a preferred homing site for breast cancer metastasis. To date, treatment options patients with bone metastases are mostly palliative and the disease still incurable. Indeed, key mechanisms involved in osteotropism only partially understood due to lack of suitable animal models mimic metastasis human tumor cells microenvironment. In presented study, we investigate use tissue-engineered construct develop humanized xenograft model cancer-induced murine host. Primary...
Abstract The mechanical properties of cancer cells and their microenvironment contribute to breast progression. While mechanosensing has been extensively studied using 2D substrates, much less is known about it in a physiologically more relevant 3D context. Here demonstrated that tumor spheroids, growing polyethylene glycol‐heparin hydrogels, are sensitive environment stiffness. During spheroid growth, compressive stresses up 2 kPa build up, as quantitated elastic polymer beads stress...
Standardized polyacrylamide microgel beads as novel tools to calibrate experiments in biomechanics and measure stresses complex tissues.
To divide in a tissue, both normal and cancer cells become spherical mechanically stiffen as they enter mitosis. We investigated the effect of oncogene activation on this process epithelial cells. found that short-term induction oncogenic Ras
Quantitative measurements of physical parameters become increasingly important for understanding biological processes. Brillouin microscopy (BM) has recently emerged as one technique providing the 3D distribution viscoelastic properties inside samples − so far relying on implicit assumption that refractive index (RI) and density can be neglected. Here, we present a novel method (FOB microscopy) combining BM with optical diffraction tomography epifluorescence imaging explicitly measuring...
Abstract The levels of nuclear protein Lamin A/C are crucial for mechanotransduction. known to scale with tissue stiffness and extracellular matrix in mesenchymal tissues. But epithelial tissues, where cells lack a strong interaction the matrix, it is unclear how regulated. Here, we show tissues that apico-basal cell compression, independent stiffness. Using genetic perturbations Drosophila compression regulates by deforming nucleus. Further, mammalian cells, deformation modulating...
Biophysical and biochemical properties of the microenvironment regulate cellular responses such as growth, differentiation, morphogenesis migration in normal cancer cells. Since two-dimensional (2D) cultures lack essential characteristics native microenvironment, three-dimensional (3D) have been developed to better mimic natural extracellular matrix. To date, 3D culture systems relied mostly on collagen Matrigel™ hydrogels, allowing only limited control over matrix stiffness, proteolytic...
Abstract Adipose tissue expansion involves both differentiation of new precursors and size increase mature adipocytes. While the two processes are well balanced in healthy tissues, obesity diabetes type II associated with abnormally enlarged adipocytes excess lipid accumulation. Previous studies suggested a link between cell stiffness, volume stem differentiation, although context preadipocytes, there have been contradictory results regarding stiffness changes differentiation. Thus, we set...
The establishment of cell adhesion involves specific recognition events between individual cell-surface receptors and molecules the cellular environment. However, characterizing single-molecule in context a living presents an experimental challenge. atomic force microscope (AFM) operated spectroscopy mode provides ultrasensitive method to investigate forces at level single receptor-ligand bonds. With attached AFM cantilever, number cell-substrate interactions can be controlled limited...