A5079111455- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- Microfluidic and Bio-sensing Technologies
- Caveolin-1 and cellular processes
- Hippo pathway signaling and YAP/TAZ
- 3D Printing in Biomedical Research
- RNA Research and Splicing
- Thin-Film Transistor Technologies
- Nanofabrication and Lithography Techniques
- Microfluidic and Capillary Electrophoresis Applications
- Advancements in Photolithography Techniques
- Nanopore and Nanochannel Transport Studies
- Neural Networks and Applications
- Metallurgy and Material Forming
- Skin and Cellular Biology Research
- Advanced MEMS and NEMS Technologies
- Integrated Circuits and Semiconductor Failure Analysis
- Microtubule and mitosis dynamics
- Digital Imaging for Blood Diseases
- Autophagy in Disease and Therapy
- Bioinformatics and Genomic Networks
- Genetics, Bioinformatics, and Biomedical Research
- Advanced Surface Polishing Techniques
- Force Microscopy Techniques and Applications
- Immunotherapy and Immune Responses
TU Dresden
2017-2025
Max Planck Institute for the Science of Light
2019-2025
University of Cambridge
2024-2025
Significance Tension is the force-opposing stretch of lipid membranes. It controls cell functions involving Membranes rupture above a tension threshold, causing death if not properly buffered. However, how membrane quantitatively regulated unknown because it difficult to measure. Using fluorescent probe, we explored coupling between and volume changes during osmosis. This described by an equilibrium theory linking folding unfolding membrane. nevertheless actively components such as...
Cell mechanical properties determine many physiological functions, such as cell fate specification, migration, or circulation through vasculature. Identifying factors that govern the is therefore a subject of great interest. Here, we present mechanomics approach for establishing links between single-cell phenotype changes and genes involved in driving them. We combine characterization cells across variety mouse human systems with machine learning-based discriminative network analysis...
Cellular reprogramming is a dedifferentiation process during which cells continuously undergo phenotypical remodeling. Although the genetic and biochemical details of this remodeling are fairly well understood, little known about change in cell mechanical properties process. In study, we investigated changes phenotype murine fetal neural progenitor (fNPCs) to induced pluripotent stem (iPSCs). We find that fNPCs become progressively stiffer en route pluripotency, stiffening mirrored by iPSCs...
The nutrient-activated mTORC1 (mechanistic target of rapamycin kinase complex 1) signaling pathway determines cell size by controlling mRNA translation, ribosome biogenesis, protein synthesis, and autophagy. Here, we show that vimentin, a cytoskeletal intermediate filament have known to be important for wound healing cancer progression, through signaling, an effect is also manifested at the organism level in mice. This vimentin-mediated regulation all levels mTOR downstream activation...
Animal cells undergo repeated shape changes, for example by rounding up and respreading as they divide. Cell can be also observed in interphase cells, when cancer switch from a mesenchymal to an ameboid mode of cell migration. Nevertheless, it remains unclear how round up. In this article, we demonstrate that partial loss substrate adhesion triggers actomyosin-dependent cortical remodeling ERM activation, which facilitates further causing round. Although the path case superficially resembles...
Abstract Dendritic cells use amoeboid migration through constricted passages to reach the lymph nodes, and this homing function is crucial for immune responses. Amoeboid requires mechanical resilience, however, underlying molecular mechanisms type of remain unknown. Because vimentin intermediate filaments (IFs) microfilaments regulate adhesion-dependent in a bidirectional manner, we analyzed if they exert similar control on migration. Vimentin was required cellular via joint interaction...
Cell mechanical properties determine many physiological functions, such as cell fate specification, migration, or circulation through vasculature. Identifying factors that govern the is therefore a subject of great interest. Here we present mechanomics approach for establishing links between single-cell phenotype changes and genes involved in driving them. We combine characterization cells across variety mouse human systems with machine learning-based discriminative network analysis...
Abstract Cytoskeletal motors transform chemical energy into mechanical work to drive essential cellular functions. Optical trapping experiments have provided crucial insights the operation of these molecular machines under load. However, throughput such force spectroscopy is typically limited one measurement at a time. Here, highly‐parallel, microfluidics‐based method that allows for rapid collection force‐dependent motility parameters cytoskeletal with two orders magnitude improvement in...
Identification of different cell types is an indispensable part in biomedical research and clinical application. During the last decades, much attention was put onto molecular characterization many can now be identified sorted based on established markers. The required staining process a lengthy costly treatment, which cause alterations cellular properties, contaminate sample therefore limit its subsequent use. A promising alternative to markers label-free identification cells using...
The identification and separation of specific cells from heterogeneous populations is an essential prerequisite for further analysis or use. Conventional passive active approaches rely on fluorescent magnetic tags introduced to the interest through molecular markers. Such labeling time- cost-intensive, can alter cellular properties, might be incompatible with subsequent use, example, in transplantation. Alternative label-free utilizing morphological mechanical features are attractive, but...
Cell mechanical properties determine many physiological functions, such as cell fate specification, migration, or circulation through vasculature. Identifying factors that govern the is therefore a subject of great interest. Here we present mechanomics approach for establishing links between single-cell phenotype changes and genes involved in driving them. We combine characterization cells across variety mouse human systems with machine learning-based discriminative network analysis...
Cell mechanical properties determine many physiological functions, such as cell fate specification, migration, or circulation through vasculature. Identifying factors that govern the is therefore a subject of great interest. Here, we present mechanomics approach for establishing links between single-cell phenotype changes and genes involved in driving them. We combine characterization cells across variety mouse human systems with machine learning-based discriminative network analysis...
Abstract Dendritic cells use amoeboid migration to pass through confined tissues reach the lymph nodes, and this homing function is crucial for immune responses. The underlying mechanisms type of remain unknown. As vimentin intermediate filaments regulate adhesion-dependent migration, we analyzed whether they have a similar effect on migration. We show that lack impairs in vitro environments, blocks lymph-node mice vivo . Importantly, vimentin-deficient dendritic lower coupling factor...
ABSTRACT During osmotic changes of their environment, cells actively regulate volume and plasma membrane tension that can passively change through osmosis. How are coupled during adaptation remains unknown, as a quantitative characterization is lacking. Here, we performed dynamic cell measurements shocks. the first few seconds following shock, varied to equilibrate pressures inside outside cell, dynamically followed these changes. A theoretical model based on passive, reversible unfolding it...
Abstract Cell mechanical properties determine many physiological functions, such as cell fate specification, migration, or circulation through vasculature. Identifying factors that govern the is therefore a subject of great interest. Here we present mechanomics approach for establishing links between single-cell phenotype changes and genes involved in driving them. We combine characterization cells across variety mouse human systems with machine learning-based discriminative network analysis...
Abstract Cytoskeletal motors transform chemical energy into mechanical work to drive essential cellular functions. Optical trapping experiments have provided crucial insights the operation of these molecular machines under load. However, throughput such force spectroscopy is typically limited one measurement at a time. Here, we introduce highly-parallel, microfluidics-based method that allows for rapid collection force-dependent motility parameters cytoskeletal with two orders magnitude...