A5028458234- Cellular Mechanics and Interactions
- Microfluidic and Bio-sensing Technologies
- Cell Image Analysis Techniques
- 3D Printing in Biomedical Research
- Hippo pathway signaling and YAP/TAZ
- Force Microscopy Techniques and Applications
- Digital Imaging for Blood Diseases
- RNA Research and Splicing
- Genetics, Bioinformatics, and Biomedical Research
- Advanced Fluorescence Microscopy Techniques
- Caveolin-1 and cellular processes
- Nerve injury and regeneration
- Microfluidic and Capillary Electrophoresis Applications
- Adipose Tissue and Metabolism
- Tendon Structure and Treatment
- Blood properties and coagulation
- Microtubule and mitosis dynamics
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Materials and Mechanics
- Bioinformatics and Genomic Networks
- Cell Adhesion Molecules Research
- Adipokines, Inflammation, and Metabolic Diseases
- Digital Holography and Microscopy
- Spinal Cord Injury Research
- Tardigrade Biology and Ecology
TU Dresden
2017-2025
Max Planck Institute for the Science of Light
2019-2025
Bayer (Germany)
2024
Center for Systems Biology Dresden
2020-2022
Max Planck Institute for Physics
2019
Technion – Israel Institute of Technology
2015-2019
Biotechnology Research Center
2018
SENTECH Instruments (Germany)
2017
The mechanical properties of biological tissues are increasingly recognized as important factors in developmental and pathological processes. Most existing measurement techniques either necessitate destruction the tissue for access or provide insufficient spatial resolution. Here, we show first time to our knowledge a systematic application confocal Brillouin microscopy quantitatively map spinal cord during biologically relevant processes contact-free nondestructive manner. Living zebrafish...
Standardized polyacrylamide microgel beads as novel tools to calibrate experiments in biomechanics and measure stresses complex tissues.
Many organs are formed through folding of an epithelium. This change in shape is usually attributed to tissue heterogeneities, for example, local apical contraction. In contrast, compressive stresses have been proposed fold a homogeneous epithelium by buckling. While buckling appealing mechanism, demonstrating that it underlies requires measurement the stress field and material properties tissue, which currently inaccessible vivo. Here, we show monolayers identical cells proliferating on...
Quantitative measurements of physical parameters become increasingly important for understanding biological processes. Brillouin microscopy (BM) has recently emerged as one technique providing the 3D distribution viscoelastic properties inside samples − so far relying on implicit assumption that refractive index (RI) and density can be neglected. Here, we present a novel method (FOB microscopy) combining BM with optical diffraction tomography epifluorescence imaging explicitly measuring...
Numerous cell functions are accompanied by phenotypic changes in viscoelastic properties, and measuring them can help elucidate higher level cellular health disease. We present a high-throughput, simple low-cost microfluidic method for quantitatively the elastic (storage) viscous (loss) modulus of individual cells. Cells suspended high-viscosity fluid pumped with high pressure through 5.8 cm long 200 µm wide channel. The shear stress induces large, ear ellipsoidal deformations. In addition,...
Abstract Artificial intelligence (AI)‐based image analysis has increased drastically in recent years. However, all applications use individual solutions, highly specialized for a particular task. Here, an easy‐to‐use, adaptable, and open source software, called AIDeveloper (AID) to train neural nets (NN) classification without the need programming is presented. AID provides variety of NN‐architectures, allowing apply trained models on new data, obtain performance metrics, export final...
Abstract Adipose tissue expansion involves both differentiation of new precursors and size increase mature adipocytes. While the two processes are well balanced in healthy tissues, obesity diabetes type II associated with abnormally enlarged adipocytes excess lipid accumulation. Previous studies suggested a link between cell stiffness, volume stem differentiation, although context preadipocytes, there have been contradictory results regarding stiffness changes differentiation. Thus, we set...
Extracellular matrix (ECM) deposition after central nervous system (CNS) injury leads to inhibitory scarring in humans and other mammals, whereas it facilitates axon regeneration the zebrafish. However, molecular basis of these different fates is not understood. Here, we identify small leucine-rich proteoglycans (SLRPs) as a contributing factor failure mammals. We demonstrate that SLRPs chondroadherin, fibromodulin, lumican, prolargin are enriched rodent human but zebrafish CNS lesions....
Immune cells process a myriad of biochemical signals but their function and behavior are also determined by mechanical cues. Macrophages no exception to this. Being present in all types tissues, macrophages exposed environments varying stiffness, which can be further altered under pathological conditions. While it is becoming increasingly clear that mechanosensitive, remains poorly understood how cues modulate inflammatory response. Here we report substrate stiffness influences the...
Atomic force microscopy (AFM) is widely used for quantifying the mechanical properties of soft materials such as cells. AFM force-indentation curves are conventionally fitted with a Hertzian model to extract elastic properties. These solely are, however, insufficient describe Here, we expand analysis capabilities viscoelastic behavior while using same curves. Our gives an explicit relation and indentation extracts physically meaningful parameters. We first validated on simulated Then,...
Morphogenesis requires spatiotemporal regulation of proliferation, both by biochemical and mechanical cues. In epithelia, this is called contact inhibition but disentangling from cues remains challenging. Here, we show that epithelia growing under confinement accumulate pressure inhibits proliferation above a threshold value. During growth, spontaneously buckle, cell transiently reactivated within the fold. Reactivation folds correlated with local reactivation mechano-sensing YAP/TAZ...
Cell mechanical properties determine many physiological functions, such as cell fate specification, migration, or circulation through vasculature. Identifying factors that govern the is therefore a subject of great interest. Here, we present mechanomics approach for establishing links between single-cell phenotype changes and genes involved in driving them. We combine characterization cells across variety mouse human systems with machine learning-based discriminative network analysis...
Cellular reprogramming is a dedifferentiation process during which cells continuously undergo phenotypical remodeling. Although the genetic and biochemical details of this remodeling are fairly well understood, little known about change in cell mechanical properties process. In study, we investigated changes phenotype murine fetal neural progenitor (fNPCs) to induced pluripotent stem (iPSCs). We find that fNPCs become progressively stiffer en route pluripotency, stiffening mirrored by iPSCs...
Atomic force microscopy (AFM) allows the mechanical characterization of single cells and live tissue by quantifying force-distance (FD) data in nano-indentation experiments. One main problems when dealing with biological is fact that measured FD curves can be disturbed. These disturbances are caused, for instance, passive cell movement, adhesive forces between AFM probe cell, or insufficient attachment to supporting cover slide. In practice, resulting artifacts easily spotted an experimenter...
The quantification of physical properties biological matter gives rise to novel ways understanding functional mechanisms. One the basic biophysical is mass density (MD). It affects dynamics in sub-cellular compartments and plays a major role defining opto-acoustical cells tissues. As such, MD can be connected refractive index (RI) via well known Lorentz-Lorenz relation, which takes into account polarizability matter. However, computing based on RI measurements poses challenge, as it requires...
During obesity development, preadipocytes proliferate and differentiate into new mature adipocytes, to increase the storage capacity of triglycerides. The morphology cells changes during differentiation from an elongated spindle-shape preadipocyte a rounded, differentiated adipocyte. That change allows efficient packing spheroidal (triglyceride) lipid droplets in cells, also reducing their ability migrate. is well known. However, little known about dynamic mechanical interactions with...
Many organisms, including yeast cells, bacteria, nematodes and tardigrades, endure harsh environmental conditions, such as nutrient scarcity, or lack of water energy for a remarkably long time. The rescue programs that these organisms launch upon encountering adverse conditions include reprogramming their metabolism in order to enter quiescent dormant state controlled fashion. Reprogramming coincides with changes the macromolecular architecture physical mechanical properties cells. However,...
Summary Many organs, such as the gut or spine are formed through folding of an epithelium. This change in shape is usually attributed to tissue heterogeneities, for example, local apical contraction. In contrast, compressive stresses have been proposed fold a homogeneous epithelium by buckling. While buckling appealing mechanism, demonstrating that it underlies requires measure stress field and material properties tissue, which currently inaccessible vivo . Here we show monolayers identical...
Cell mechanical properties determine many physiological functions, such as cell fate specification, migration, or circulation through vasculature. Identifying factors that govern the is therefore a subject of great interest. Here we present mechanomics approach for establishing links between single-cell phenotype changes and genes involved in driving them. We combine characterization cells across variety mouse human systems with machine learning-based discriminative network analysis...
The quantification of physical properties biological matter gives rise to novel ways understanding functional mechanisms by utilizing models that explicitly depend on observables. One the basic biophysical is mass density (MD), which determines degree crowdedness. It impacts dynamics in sub-cellular compartments and further plays a major role defining opto-acoustical cells tissues. As such, MD can be connected refractive index (RI) via well known Lorentz-Lorenz relation, takes into account...
Abstract The mechanical properties of biological tissues are increasingly recognized as important factors in developmental and pathological processes. Most existing measurement techniques either necessitate destruction the tissue for access or provide insufficient spatial resolution. Here, we show first time a systematic application confocal Brillouin microscopy to quantitatively map spinal cord during biologically relevant processes contact-free non-destructive manner. Living zebrafish...