A5048871673- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Digital Imaging for Blood Diseases
- Hippo pathway signaling and YAP/TAZ
- Skin and Cellular Biology Research
- melanin and skin pigmentation
- Radiomics and Machine Learning in Medical Imaging
- Systemic Sclerosis and Related Diseases
- Cancer Cells and Metastasis
- Genetics, Bioinformatics, and Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Hair Growth and Disorders
- Force Microscopy Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Genital Health and Disease
- Mathematical Biology Tumor Growth
- Cancer Research and Treatment
- Caveolin-1 and cellular processes
- Lymphatic System and Diseases
- Blood properties and coagulation
- Renal and related cancers
- Cardiomyopathy and Myosin Studies
- Cell death mechanisms and regulation
Max Planck Institute for the Science of Light
2019-2023
Technion – Israel Institute of Technology
2016-2023
Manchester University NHS Foundation Trust
2017
University of Manchester
2013-2017
Manchester Academic Health Science Centre
2017
During surgery, rapid and accurate histopathological diagnosis is essential for clinical decision making. Yet the prevalent method of intra-operative consultation pathology intensive in time, labour costs, requires expertise trained pathologists. Here we show that biopsy samples can be analysed within 30 min by sequentially assessing physical phenotypes singularized suspended cells dissociated from tissues. The diagnostic combines enzyme-free mechanical dissociation tissues, real-time...
Highlights•K15-GFP marks the stem cell (SC) and boundary compartment of corneal epithelium•SC depletion is restored by dedifferentiation committed cells•Damage to SC niche abolished recovery from loss•Direct evidence for conjunctival invasion into cornea in pathologySummaryDuring morphogenesis, preserving tissue boundaries essential fate regulation. While embryonic tissues possess high plasticity repair ability, questions whether how adult cope with acute loss or disruption have remained...
Increased apoptotic shedding has been linked to intestinal barrier dysfunction and development of inflammatory bowel diseases (IBD). In contrast, physiological cell allows the renewal epithelial monolayer without compromising function. Here, we investigated role live extrusion in alterations IBD.Taking advantage conditional GGTase RAC1 knockout mice cells (Pggt1biΔIEC Rac1iΔIEC mice), intravital microscopy, immunostaining, mechanobiology, organoid techniques RNA sequencing, analysed within...
Sorting cells is an essential primary step in many biological and clinical applications such as high-throughput drug screening, cancer research cell transplantation. Cell sorting based on their mechanical properties has long been considered a promising label-free biomarker that could revolutionize the isolation of from heterogeneous populations. Recent advances microfluidic image-based analysis combined with subsequent by on-chip actuators demonstrated possibility physical properties....
Alzheimer's disease (AD) is characterized by toxic protein accumulation in the brain. Ubiquitination essential for clearance cells, making altered ubiquitin signaling crucial AD development. A defective variant, B + 1 (UBB+1), created a non-hereditary RNA frameshift mutation, found all patient brains post-mortem. We now detect UBB+1 human during early stages. Our study employs 3D neural culture platform derived from progenitors, demonstrating that alone induces extracellular amyloid-β (Aβ)...
Abstract Artificial intelligence (AI)‐based image analysis has increased drastically in recent years. However, all applications use individual solutions, highly specialized for a particular task. Here, an easy‐to‐use, adaptable, and open source software, called AIDeveloper (AID) to train neural nets (NN) classification without the need programming is presented. AID provides variety of NN‐architectures, allowing apply trained models on new data, obtain performance metrics, export final...
Human embryonic stem cells (hESCs) are pluripotent that have indefinite replicative potential and the ability to differentiate into derivatives of all three germ layers. hESCs conventionally grown on mitotically inactivated mouse fibroblasts (MEFs) or feeder human origin. In addition, feeder-free culture systems can be used support hESCs, in which adhesive substrate plays a key role regulation cell self-renewal differentiation. Extracellular matrix (ECM) components define microenvironment...
Human embryonic stem cells (hESCs) hold tremendous promise for cell replacement therapies a range of degenerative diseases. In order to provide cost-effective treatments affordable by public health systems, HLA-matched allogeneic tissue banks the highest quality clinical-grade hESCs will be required. However only small number existing hESC lines are suitable clinical use; they limited moral and ethical concerns none them apply Good Manufacturing Practice (GMP) standards earliest critical...
Stem cells (SCs) play a pivotal role in fueling homeostasis and regeneration. While much focus has been given to self-renewal differentiation pathways regulating SC fate, little is known regarding the specific mechanisms utilized for their elimination. Here, we report that pro-apoptotic protein ARTS (a Septin4 isoform) highly expressed comprising intestinal niche its deletion protects Lgr5+ Paneth from undergoing apoptotic cell death. As result, Sept4/ARTS-/- crypt displays augmented...
Atomic force microscopy (AFM) is widely used for quantifying the mechanical properties of soft materials such as cells. AFM force-indentation curves are conventionally fitted with a Hertzian model to extract elastic properties. These solely are, however, insufficient describe Here, we expand analysis capabilities viscoelastic behavior while using same curves. Our gives an explicit relation and indentation extracts physically meaningful parameters. We first validated on simulated Then,...
The hair follicle (HF) is an ideal system for studying the biology and regulation of adult stem cells (SCs). This dynamic mini organ replenished by distinct pools SCs, which are located in permanent portion HF, a region known as bulge. These multipotent bulge SCs were initially identified slow cycling label retaining cells; however, their isolation has been made feasible after identification specific cell markers, such CD34 keratin 15 (K15). Here, we describe robust method isolating...
The hair follicle (HF) is an ideal system for studying the biology and regulation of adult stem cells (SCs). This dynamic mini organ replenished by distinct pools SCs, which are located in permanent portion HF, a region known as bulge. These multipotent bulge SCs were initially identified slow cycling label retaining cells; however, their isolation has been made feasible after identification specific cell markers, such CD34 keratin 15 (K15). Here, we describe robust method isolating...
The epidermis consists of several distinct compartments including the interfollicular (IFE), sweat glands, sebaceous glands (SGs), and hair follicle (HF). While IFE SGs are in a constant state self-renewal, HF cycles between phases growth, destruction, rest. stem cells (HFSCs) that fuel this perpetual cycle have been well described located niche termed bulge. These bulge SCs express markers such as CD34 Keratin 15 (K15), enabling isolation these cells. Here, we describe powerful method for...
Objective Pathologically activated circulating immune cells, including monocytes, play major roles in systemic sclerosis (SSc). Their functional characterization can provide crucial information with direct clinical relevance. However, tools for the evaluation of pathologic cell activation and, general, outcomes SSc are scarce. Biophysical phenotyping (including mechanics and morphology) provides access to a novel, mostly unexplored layer regarding pathophysiologic activation. We hypothesized...
We present a novel 3D colloidal crystal made of polyacrylamide microgel beads with variable stiffness. The can be functionalized ECM proteins and used to study mechanosensing responses cells varying stiffness in context.
Abstract Publications on artificial intelligence (AI)-based image analysis have increased drastically in recent years. However, all applications use individual solutions highly specialized for a particular task. Here, we present an easy-to-use, adaptable, open source software, called AIDeveloper (AID) to train neural nets (NN) classification without the need programming. The software provides variety of NN-architectures that can be simply selected training. AID allows user apply trained...
Abstract Rapid and accurate histopathological diagnosis during surgery is critical for clinical decision-making. The prevalent method of intraoperative consultation pathology time, labour cost intensive requires the expertise trained pathologists. Here, we present an alternative technique rapid, label-free analysis biopsy samples by sequentially assessing physical phenotype singularized, suspended cells in high-throughput. This new diagnostic pipeline combines enzyme-free, mechanical...
Background: Systemic sclerosis (SSc) is associated with high morbidity and one of the autoimmune rheumatic diseases highest mortality. However, tools to evaluate disease activity, response treatment or predict progression are scarce. Dysregulated immune responses major pathogenic players at onset in SSc. Recent evidence demonstrates that mechanical properties circulating leukocytes reflect their states functions, during activation ensure adaptation changing physical requirements (e.g....
ABSTRACT Introduction A fundamental mechanism for proper development and tissue homeostasis is apoptosis, which responsible the elimination of undesired potentially dangerous cells. Apoptosis culminates in activation caspase-3, plays an important role implementing cell death program. Material methods Performing both vivo vitro studies we reveal a surprising non-apoptotic caspase-3 as key regulator proliferation, organ size tumour development. Results discussions Here, Caspase-3 specifically...