A5080954775- Innovative Microfluidic and Catalytic Techniques Innovation
- 3D Printing in Biomedical Research
- Microfluidic and Capillary Electrophoresis Applications
- Hydrogels: synthesis, properties, applications
- Electrowetting and Microfluidic Technologies
- Pickering emulsions and particle stabilization
- Additive Manufacturing and 3D Printing Technologies
- Radiation Therapy and Dosimetry
- Cellular Mechanics and Interactions
- Supramolecular Self-Assembly in Materials
- Electrohydrodynamics and Fluid Dynamics
- Lipid Membrane Structure and Behavior
- Polymer Nanocomposites and Properties
- Advanced biosensing and bioanalysis techniques
- Advanced Radiotherapy Techniques
- Advanced Materials and Mechanics
- Tissue Engineering and Regenerative Medicine
- Advanced Polymer Synthesis and Characterization
- Polymer composites and self-healing
- Microfluidic and Bio-sensing Technologies
- Advanced Sensor and Energy Harvesting Materials
- Atomic and Subatomic Physics Research
- Proteoglycans and glycosaminoglycans research
- Pancreatic function and diabetes
- RNA and protein synthesis mechanisms
Leibniz Institute of Polymer Research
2015-2025
Otto-von-Guericke University Magdeburg
2022-2025
Leibniz University Hannover
2023
TU Dresden
1893-2022
University Hospital Carl Gustav Carus
2016-2021
OncoRay
2015-2021
Integra (United States)
2019
Software (Spain)
2019
Halo Labs (United States)
2018
Systems Technology (United States)
2018
Here, we present a platform to detect cytokine (IL-2, IFN-γ, TNF-α) secretion of single, activated T-cells in droplets over time. We use novel droplet-based microfluidic approach encapsulate cells monodisperse agarose together with functionalized cytokine-capture beads for subsequent binding and detection secreted cytokines from single cells. This method allows high-throughput cellular heterogeneity maps subsets within cell populations specific functions.
We describe new developments for controlled fabrication of monodisperse non-spherical particles using droplet microfluidics. The high degree control afforded by microfluidic technologies enables generation single and multiple emulsion droplets. show that these droplets can be transformed to through further simple, spontaneous processing steps, including arrested coalescence, asymmetric polymer solidification, polymerization in flow, evaporation-driven clustering. These versatile scalable...
We present a robust way to create multiple emulsions with controllable shell thicknesses that can vary over wide range. use microfluidic device coaxial jet of immiscible fluids; using dripping instability, we break the into emulsions. By controlling thickness each layer jet, adjust shells The same method is also effective in creating monodisperse from fluids cannot otherwise be controllably emulsified, such as, for example, viscoelastic fluids.
Microgel particles and capsules which consist of multiple layers can be fabricated using droplet microfluidics, but in existing methods, emulsion templating forms dissimilar polarity. In this paper, we fabricate functional microgel that two miscible yet distinct layers. We use microfluidic devices to template micrometer-sized drops are loaded with prepolymerized precursors solidify them through a polymer-analogous reaction. This allows the particle morphology controlled prevents pronounced...
The flow orientation of anisotropic particles through narrow channels is importance in many fields, ranging from the spinning and molding fibers to cells proteins thin capillaries. It commonly assumed that align parallel direction. When flowing narrowed channel sections, one expects increased rate improve alignment. Here, we show by microfocus synchrotron X-ray scattering polarized optical microscopy colloidal perpendicular direction after passing a section. We find this be general behavior...
Microgel particles are formed from aqueous-two-phase-system (ATPS) droplets in poly(dimethylsiloxane) (PDMS) microfluidic devices. The consist of a dextran core and photopolymerizable poly(ethylene glycol) diacrylate (PEGDA) shell. Upon UV exposure, the ATPS undergo shape-transformation yielding PEGDA microgel containing socket. Detailed facts importance to specialist readers published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made...
We present a simple method to spatially pattern the surface properties of microfluidic devices using flow confinement. Our technique allows patterning with micron-scale resolution. To demonstrate its effectiveness, we use it wettability form W/O/W and O/W/O double emulsions.
Standardized polyacrylamide microgel beads as novel tools to calibrate experiments in biomechanics and measure stresses complex tissues.
Like charges stabilize emulsions, whereas opposite break emulsions. This is the fundamental principle for many industrial and practical processes. Using micrometer-sized pH-sensitive polymeric hydrogel particles as emulsion stabilizers, we prepare emulsions that consist of oppositely charged droplets, which do not coalesce. We observe noncoalescence droplets in bulk emulsification well microfluidic devices, where are forced to collide within channel junctions. The results demonstrate...
The formation of polymersomes from copolymer-stabilized double emulsions in glass-coated, PDMS microfluidic devices is described. device geometry enables separate injection two organic solvents to form the shell phase emulsion. This allows proper combination solvents, which essential for forming polymersomes.
In droplet-based microfluidics, non-ionic, high-molecular weight surfactants are required to stabilize droplet interfaces. One of the most common structures that imparts stability as well biocompatibility water-in-oil droplets is a triblock copolymer surfactant composed perfluoropolyether (PFPE) and polyethylene glycol (PEG) blocks. However, fast growing applications microdroplets in biology would benefit from larger choice specialized surfactants. PEG hydrophilic moiety, however, very...
We demonstrate that microfluidic flow devices enable a rapid, continuous, well-reproducible and size-controlled preparation of unilamellar block copolymer vesicles. The PDMS-based device consists perpendicularly crossed channels allowing hydrodynamic focusing an ethanolic solution in stream water. By altering the rate ratio water inlet channels, vesicle size can be tuned over large range from 40 nm to 2 μm without subsequent processing steps manipulating shell characteristics. ability tuning...
A series of water-soluble macro-initiators is synthesized to avoid radical loss in microfluidic on-chip photo cross-linking hyaluronic acid methacrylate-containing water-in-oil emulsions. Their superior performance over known photo-initiators through the generation radicals and excellent biocompatibility are demonstrated.
Abstract Cell‐like platforms are being studied intensively for their application in synthetic biology to mimic aspects of life an artificial environment. Here, micrometer‐sized, bifunctional microgels used as experimental platform investigate the interplay cell‐free protein synthesis (CFPS) and situ accumulation inside microgel volume. In detail, made hyaluronic acid (HA) first modified with different amounts nitrilotriacetic (NTA) moieties characterize capability maximum capacity binding...
We microfluidically fabricate bio-orthogonal DNA-functionalized porous hydrogels from hyaluronic acid that are employed in vitro transcription/translation (IVTT) of a green fluorescent protein. By co-encapsulating individual hydrogel particles and the IVTT machinery water-in-oil microdroplets, we study protein expression defined reaction volume. Our approach enables precise control over rates by gene dosage. show transcription translation confined to membrane-free matrix, which contributes...
Mesenchymal stem cells are encapsulated in a 3D fibrinogen–hyaluronic acid culture through droplet microfluidics, whose morphology, multipotency and differentiation studied.
Abstract The fabrication of microfluidic devices with nonplanar microchannel design by micro‐stereolithography (µSL) for oil‐in‐water (O/W) and water‐in‐oil (W/O) single emulsion as well oil‐in‐water‐in‐oil (O/W/O) water‐in‐oil‐in‐water (W/O/W) double formation is presented. By investigating separation distance, printing direction voxel compensation in X , Y ‐plane key parameters, microflow cells channel cross‐sections varying from 500 to 75 µm are printed, which thus truly similar those...
We develop resins for high-resolution additive manufacturing of flexible micromaterials via projection microstereolithography (PμSL) screening formulations made from monomer 2-phenoxyethyl acrylate, the cross-linkers Ebecryl 8413, tri(propyleneglycol) diacrylate or 1,3,5-triallyl-1,3,5-triazine-2,4,6(1H,3H,5H)-trione, photoabsorber Sudan 1, and photoinitiator diphenyl(2,4,6-trimethylbenzoyl)phosphine oxide. PμSL-printed polymer this resin library are characterized regarding achievable layer...
Early development drug formulation is exacerbated by increasingly poor bioavailability of potential candidates. Prevention attrition due to problems necessitates physicochemical analysis and studies at a very early stage during development, where the availability new substance limited small quantities, thus impeding extensive experiments. Miniaturization common processes strategy overcome those limitations. We present versatile technique for fabricating nanoformulations using microfluidic...
Over the last two decades, droplet‐based microfluidics has evolved into a versatile tool for fabricating tailored micrometer‐sized hydrogel particles. Combining precise fluid handling down to femtoliter scale with diverse precursor design, it allows excellent control over microgel size and shape, but also functionalization crosslinking density. Consequently, is possible tune physicochemical mechanical properties such as swelling, degradation, stimuli sensitivity, elasticity by microfluidic...
Polysaccharide-based microgels have broad applications in multi-parametric cell cultures, cell-free biotechnology, and drug delivery. Multicomponent reactions like the Passerini three-component Ugi four-component reaction are shown here to be versatile platforms for fabricating these polysaccharide by droplet microfluidics with a narrow size distribution. While conventional microgel formation requires pre-modification of hydrogel building blocks introduce certain functionality,...
This paper describes an electro-demulsification chip for extracting cells and polymer particles into aqueous phase from microfluidic water-in-oil emulsions.
As low-molecular-weight hydrogelators, dipeptide hydrogel materials are suited for embedding multiple organic molecules and inorganic nanoparticles. Herein, a simple but precisely controllable method is presented that enables the fabrication of dipeptide-based hydrogels by supramolecular assembly inside microfluidic channels. Water-soluble quantum dots (QDs) as well premixed porphyrins in dimethyl sulfoxide (DMSO) were injected into Y-shaped junction. At DMSO/water interface, confined was...