A5057058267- Polymer Surface Interaction Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Molecular Junctions and Nanostructures
- Nanofabrication and Lithography Techniques
- Microfluidic and Capillary Electrophoresis Applications
- Organic Electronics and Photovoltaics
- Gene Regulatory Network Analysis
- Force Microscopy Techniques and Applications
- Conducting polymers and applications
- Electrowetting and Microfluidic Technologies
- 3D Printing in Biomedical Research
- Dendrimers and Hyperbranched Polymers
- Adhesion, Friction, and Surface Interactions
- Photoreceptor and optogenetics research
- Surface Modification and Superhydrophobicity
- Cellular Mechanics and Interactions
- Advanced Polymer Synthesis and Characterization
- Micro and Nano Robotics
- Origins and Evolution of Life
- Pickering emulsions and particle stabilization
- Protein Structure and Dynamics
- Lipid Membrane Structure and Behavior
- Supramolecular Self-Assembly in Materials
- RNA and protein synthesis mechanisms
- Advanced Fluorescence Microscopy Techniques
Radboud University Nijmegen
2016-2025
Radboud Institute for Molecular Life Sciences
2013-2024
Radboud University Medical Center
2013-2024
Sichuan University
2022
University of Electronic Science and Technology of China
2022
Hunan University
2022
University of Cambridge
2007-2017
Box (United States)
2015
Institute of Physical and Organic Chemistry
2015
Lanzhou Institute of Chemical Physics
2006-2013
This letter describes a technique for generating waves on polydimethylsiloxane (PDMS) patterned in bas-relief. The PDMS is heated, and its surface oxidized an oxygen plasma; this oxidation forms thin, stiff silicate layer the surface. When cools, it contracts places under compressive stress. stress relieved by buckling to form patterns of with wavelengths from 0.5 10 μm. are locally ordered near step or edge PDMS. wavelength, amplitude, pattern can be controlled controlling temperature...
We report the first systematic study of initiator density on surface-initiated polymerizations. used mixed monolayers comprising undecanethiol and ω-mercaptoundecyl bromoisobutyrate (1) to initiate controlled radical polymerization methyl methacrylate (MMA) glycidyl (GMA) from gold. found that SAMs composed 10% 50% 1 grow PMMA brushes approximately 1/10 1/2 thickness polymer grafted 100% 1. did not find a clear maximum density, beyond which no further increase in brush is observed. show at...
This paper describes the spontaneous formation of patterns aligned buckles in a thin film gold deposited on surface an elastomer [poly(dimethylsiloxane), PDMS]. The is patterned photochemically into areas differing stiffness and coefficient thermal expansion. while warm (T ∼ 100 °C). On cooling, shrinkage places under compressive stress. relieve this distribution stresses buckle described during pre- postbuckling regimes using solutions from calculations describing model comprising stiff...
Liquid-liquid phase transitions in complex mixtures of proteins and other molecules produce crowded compartments supporting vitro transcription translation. We developed a method based on picoliter water-in-oil droplets to induce coacervation Escherichia coli cell lysate follow gene expression under noncrowded conditions. Coacervation creates an artificial cell-like environment which the rate mRNA production is increased significantly. Fits measured rates show two orders magnitude larger...
Vesosomes are nested liposomal structures with high potential as advanced drug delivery vehicles, bioreactors and artificial cells. However, to date no method has been reported prepare monodisperse vesosomes of controlled size. Here we report on a multistep microfluidic strategy for hierarchically assembling uniform from dewetting double emulsion templates. The control afforded by our is illustrated the formation concentric, pericentric multicompartment liposomes. route offers an exceptional...
In this paper, we demonstrate a double nanoimprinting process that allows the formation of nanostructured polymer heterojunctions composition and morphology can be selected independently. We fabricated photovoltaic (PV) devices with extremely high densities (1014/mm2) interpenetrating nanoscale columnar features in active blend layer. The smallest feature sizes are as small 25 nm on 50 pitch, which results spacing at or below exciton diffusion length. Photovoltaic based double-imprinted...
Liposomes are self-assembled phospholipid vesicles with great potential in fields ranging from targeted drug delivery to artificial cells. The formation of liposomes using microfluidic techniques has seen considerable progress, but the process itself not been studied detail. As a result, high throughput, high-yielding routes monodisperse multiple compartments have demonstrated. Here, we report on surfactant-assisted route uniform, single bilayer liposomes, 25 190 μm, and or without inner...
Here, we present a platform to detect cytokine (IL-2, IFN-γ, TNF-α) secretion of single, activated T-cells in droplets over time. We use novel droplet-based microfluidic approach encapsulate cells monodisperse agarose together with functionalized cytokine-capture beads for subsequent binding and detection secreted cytokines from single cells. This method allows high-throughput cellular heterogeneity maps subsets within cell populations specific functions.
Abstract Coacervates have been widely studied as model compartments in protocell research. Complex coacervates composed of disordered proteins and RNA also shown to play an important role cellular processes. Herein, we report on a microfluidic strategy for constructing monodisperse coacervate droplets encapsulated within uniform unilamellar liposomes. These structures represent bottom‐up approach hierarchically structured protocells, demonstrated by storage release DNA from the well...
We report a microfluidic droplet-based approach enabling the measurement of chemical reactions individual enzyme molecules and its application to single-molecule-counting immunoassay. A device is used generate manipulate <10 fL droplets at rates up 1.3 × 10(6) per second, about 2 orders magnitude faster than has previously been reported. The femtodroplets produced with this can be encapsulate single biomolecular complexes tagged reporter enzyme; their small volume enables fluorescent product...
Geometrical cues have been shown to alter gene expression and differentiation on 2D substrates. However, little is known about how geometrical affect cell function in 3D. One major reason for this lack of understanding rooted the difficulties controlling geometry a complex 3D setting long periods culture. Here, we present robust method control volume shape individual human mesenchymal stem cells (hMSCs) inside microniches with range different geometries (e.g., cylinder, triangular prism,...
Recent studies have shown that the interactions between condensates and biological membranes are of functional importance. Here, we study how interaction complex coacervates liposomes as model systems can lead to wetting, membrane deformation, endocytosis. Depending on strength liposomes, wetting behavior ranged from nonwetting engulfment (endocytosis) complete wetting. Endocytosis was found be a general phenomenon: made wide range components could taken up by liposomes. A simple theory...