A5035604215- RNA and protein synthesis mechanisms
- Lipid Membrane Structure and Behavior
- Polymer Surface Interaction Studies
- Pickering emulsions and particle stabilization
- Origins and Evolution of Life
- Philosophy and History of Science
- CRISPR and Genetic Engineering
- ATP Synthase and ATPases Research
- Photosynthetic Processes and Mechanisms
- Evolution and Science Education
- Photoreceptor and optogenetics research
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
Radboud University Nijmegen
2022-2024
Konrad Lorenz Institute for Evolution and Cognition Research
2024
Radboud Institute for Molecular Life Sciences
2024
Radboud University Medical Center
2024
Recent studies have shown that the interactions between condensates and biological membranes are of functional importance. Here, we study how interaction complex coacervates liposomes as model systems can lead to wetting, membrane deformation, endocytosis. Depending on strength liposomes, wetting behavior ranged from nonwetting engulfment (endocytosis) complete wetting. Endocytosis was found be a general phenomenon: made wide range components could taken up by liposomes. A simple theory...
Biomolecular condensates are a promising platform for synthetic cell formation and constitute potential missing link between the chemical cellular stage of origins life. However, it has proven challenging to integrate complex reaction networks into biomolecular condensates, such as cell-free in vitro transcription–translation (IVTT) system. Integrating IVTT successfully is one precondition condensation-based formation. Moreover, would provide proof concept that principle compatible with...
The role of evolutionary theory at the origin life is an extensively debated topic. and early development usually separated into a prebiotic phase protocellular phase, ultimately leading to Last Universal Common Ancestor. Most likely, Ancestor was subject Darwinian evolution, but question remains what extent evolution applies phases. In this review, we reflect on current status in origins research by bringing together philosophy science, biology, empirical field. We explore various ways...
Gene expression is a fundamental aspect in the construction of minimal synthetic cell, and use chromosomes will be crucial for integration regulation complex modules. Expression from vitro transcription translation (IVTT) systems presents limitations, as their large size low concentration make them far less suitable standard IVTT reactions. Here, we addressed these challenges by optimizing lysate-based at template concentrations. We then applied an active learning tool to adapt DNA. Further...
Abstract Discussions on the status of definitions life have long been dominated by a position known as definitional pessimism. Per pessimist, there is no point in trying to define life. This claim defended different ways, but one shared assumptions all pessimists that our attempts are provide list necessary and sufficient conditions for something count alive. In other words, definition strict, descriptive definition. Against this, several pragmatic alternatives put forward. On these...
Giant unilamellar vesicles (GUVs) provide a powerful model compartment for synthetic cells. However, key challenge is the incorporation of membrane proteins that allow transport, energy transduction, growth and division. Here, we have successfully incorporated protein complex SecYEG-the bacterial translocase essential newly synthesized proteins-in GUVs. Our method consists fusion small containing reconstituted SecYEG into GUVs, thereby forming SecGUVs. These are suitable large-scale...
Recent studies have shown that the interactions between condensates and biological membranes is of functional importance. Here, we study how interaction complex coacervates liposomes as model systems can lead to membrane deformation endocytosis. Depending on strength liposomes, wetting behavior ranged from non-wetting, partial (adhesion), engulfment (endocytosis), finally complete wetting. Endocytosis was found be a general phenomenon: made wide range components could taken up by liposomes....