A5038508718- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Microfluidic and Bio-sensing Technologies
- Digital Holography and Microscopy
- 3D Printing in Biomedical Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Tendon Structure and Treatment
- Nerve injury and regeneration
- Signaling Pathways in Disease
- Osteoarthritis Treatment and Mechanisms
- Proteoglycans and glycosaminoglycans research
- Acoustic Wave Resonator Technologies
- Mechanical and Optical Resonators
- Heat shock proteins research
- Sports Dynamics and Biomechanics
- Spaceflight effects on biology
- RNA Research and Splicing
- Advanced Materials and Mechanics
- Corneal Surgery and Treatments
- Photoacoustic and Ultrasonic Imaging
- Protein Structure and Dynamics
- Advanced MEMS and NEMS Technologies
- Advanced Sensor and Energy Harvesting Materials
- Hydrogels: synthesis, properties, applications
- Connective tissue disorders research
TU Dresden
2018-2024
Max Planck Institute for the Science of Light
2020-2024
Center for Systems Biology Dresden
2021
Biotechnology Research Center
2020
Quantitative measurements of physical parameters become increasingly important for understanding biological processes. Brillouin microscopy (BM) has recently emerged as one technique providing the 3D distribution viscoelastic properties inside samples − so far relying on implicit assumption that refractive index (RI) and density can be neglected. Here, we present a novel method (FOB microscopy) combining BM with optical diffraction tomography epifluorescence imaging explicitly measuring...
Extracellular matrix (ECM) deposition after central nervous system (CNS) injury leads to inhibitory scarring in humans and other mammals, whereas it facilitates axon regeneration the zebrafish. However, molecular basis of these different fates is not understood. Here, we identify small leucine-rich proteoglycans (SLRPs) as a contributing factor failure mammals. We demonstrate that SLRPs chondroadherin, fibromodulin, lumican, prolargin are enriched rodent human but zebrafish CNS lesions....
Altered biophysical properties of cancer cells and their microenvironment contribute to progression. While the relationship between microenvironmental stiffness cell mechanical responses has been previously studied using two-dimensional (2D) systems, much less is known about it in a physiologically more relevant 3D context particular for multicellular systems. To investigate influence on tumor spheroid mechanics, we first generated MCF-7 spheroids within matrix metalloproteinase...
The quantification of physical properties biological matter gives rise to novel ways understanding functional mechanisms. One the basic biophysical is mass density (MD). It affects dynamics in sub-cellular compartments and plays a major role defining opto-acoustical cells tissues. As such, MD can be connected refractive index (RI) via well known Lorentz-Lorenz relation, which takes into account polarizability matter. However, computing based on RI measurements poses challenge, as it requires...
Optical quantification of intracellular mass density using optical diffraction tomography (ODT) and cell mechanics Brillouin spectroscopy under 3D mechanical confinement.
Biomolecular condensates have been identified as a ubiquitous means of intracellular organization, exhibiting very diverse material properties. However, techniques to characterize these properties and their underlying molecular interactions are scarce. Here, we introduce two optical - Brillouin microscopy quantitative phase imaging (QPI) address this scarcity. We establish shift linewidth measures for average interaction dissipation strength, respectively, used QPI obtain the protein...
In processes such as development and regeneration, where large cellular tissue rearrangements occur, cell fate behaviour are strongly influenced by mechanics. While most well-established tools probing mechanical properties require an invasive sample preparation, confocal Brillouin microscopy captures parameters optically with high resolution in a contact-free label-free fashion. this work, we took advantage of tool the transparency highly regenerative axolotl to probe its vivo for first...
Stretching living cells with light is a key method to assess their mechanics. To estimate the heat, experience during this procedure, microgels are utilized relate temperature changes – caused by optical stretchers hydrogel swelling.
The quantification of physical properties biological matter gives rise to novel ways understanding functional mechanisms by utilizing models that explicitly depend on observables. One the basic biophysical is mass density (MD), which determines degree crowdedness. It impacts dynamics in sub-cellular compartments and further plays a major role defining opto-acoustical cells tissues. As such, MD can be connected refractive index (RI) via well known Lorentz-Lorenz relation, takes into account...
Abstract Quantitative measurements of physical parameters become increasingly important for understanding biological processes. Brillouin microscopy (BM) has recently emerged as one technique providing the 3D distribution viscoelastic properties inside samples — so far relying on implicit assumption that refractive index (RI) and density can be neglected. Here, we present a novel method (FOB microscopy) combining BM with optical diffraction tomography epi-fluorescence imaging explicitly...
Abstract Altered biophysical properties of cancer cells and their microenvironment contribute to progression. While the relationship between microenvironmental stiffness cell mechanical responses has been previously studied using two-dimensional (2D) systems, much less is known about it in a physiologically more relevant 3D context particular for multicellular systems. To investigate influence on tumor spheroid mechanics, we first generated MCF-7 spheroids within matrix metalloproteinase...
Abstract Biomolecular condensates have been identified as a ubiquitous means of intracellular organization, exhibiting very diverse material properties. However, techniques to characterize these properties and their underlying molecular interactions are scarce. Here, we introduce two optical – Brillouin microscopy quantitative phase imaging (QPI) address this scarcity. We establish shift linewidth measures for average interaction dissipation strength, respectively, used QPI obtain the...
ABSTRACT In processes such as development and regeneration, where large cellular tissue rearrangements occur, cell fate behavior are strongly influenced by mechanics. While most well-established tools probing mechanical properties require an invasive sample preparation, confocal Brillouin microscopy captures parameters optically with high resolution in a contact-free label-free fashion. this work, we took advantage of tool the transparency highly regenerative axolotl to probe its vivo for...
ABSTRACT Extracellular matrix (ECM) deposition after central nervous system (CNS) injury leads to inhibitory scarring in mammals, whereas it facilitates axon regeneration the zebrafish. However, molecular basis of these different fates is not understood. Here, we identify small leucine-rich proteoglycans (SLRPs) as a causal factor failure. We demonstrate that SLRPs Chondroadherin, Fibromodulin, Lumican, and Prolargin are enriched human, but zebrafish, CNS lesions. Targeting zebrafish ECM...
We present a combined optical system for Brillouin microscopy with diffraction tomography (ODT), which can reconstruct the three-dimensional refractive index (RI) distribution of biological samples. By correlating frequency shift reconstructed RI in same field-of-view, we calculate precise longitudinal modulus samples without priori information. demonstrate capabilities method using cell phantom consisting different hydrogel beads known mechanical properties, and apply it to quantitative...