A5073316159- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Digital Holography and Microscopy
- interferon and immune responses
- Pancreatic function and diabetes
- Genetic Neurodegenerative Diseases
- Amyotrophic Lateral Sclerosis Research
- Nuclear Structure and Function
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Diabetes Management and Research
- Biocrusts and Microbial Ecology
- Fungal and yeast genetics research
- Cell Image Analysis Techniques
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Autoimmune and Inflammatory Disorders Research
- Advanced Electron Microscopy Techniques and Applications
- Biofield Effects and Biophysics
- Endoplasmic Reticulum Stress and Disease
Indian Institute of Science Bangalore
2020-2024
TU Dresden
2020-2022
Max Planck Institute of Molecular Cell Biology and Genetics
2015-2022
National University of Singapore
2010-2017
Tata Institute of Fundamental Research
2008-2012
National Centre for Biological Sciences
2008-2012
RNA and membraneless organelles Membraneless compartments can form in cells through liquidliquid phase separation (see the Perspective by Polymenidou). But what prevents these cellular condensates from randomly fusing together? Using RNA-binding protein (RBP) Whi3, Langdon et al. demonstrated that secondary structure of different components determines distinct biophysical biological properties two types Whi3 forms. Several RBPs, such as FUS TDP43, contain prion-like domains are linked to...
Stressed cells shut down translation, release mRNA molecules from polysomes, and form stress granules (SGs) via a network of interactions that involve G3BP. Here we focus on the mechanistic underpinnings SG assembly. We show that, under non-stress conditions, G3BP adopts compact auto-inhibited state stabilized by electrostatic intramolecular between intrinsically disordered acidic tracts positively charged arginine-rich region. Upon unfolded mRNAs outcompete auto-inhibitory interactions,...
RNA-protein (RNP) granules have been proposed to assemble by forming solid RNA/protein aggregates or through phase separation into a liquid phase. Which model describes RNP in living cells is still unclear. In this study, we analyze P bodies budding yeast and find that they liquid-like properties. Surprisingly, stress adopt different material state, which reminiscent of protein controlled disaggregases. By using an assay ectopically nucleate granules, further establish granule formation does...
Cells can enter into a dormant state when faced with unfavorable conditions. However, how cells and recover from this is still poorly understood. Here, we study dormancy in different eukaryotic organisms find it to be associated significant decrease the mobility of organelles foreign tracer particles. We show that reduced caused by an influx protons marked acidification cytoplasm, which leads widespread macromolecular assembly proteins triggers transition cytoplasm solid-like increased...
Article4 April 2017Open Access Transparent process An aberrant phase transition of stress granules triggered by misfolded protein and prevented chaperone function Daniel Mateju Max Planck Institute Molecular Cell Biology Genetics, Dresden, Germany Search for more papers this author Titus M Franzmann Avinash Patel Andrii Kopach Edgar E Boczek Shovamayee Maharana Hyun O Lee Serena Carra Department Biomedical, Metabolic Neural Sciences, University Modena Reggio Emilia, Modena, Italy Anthony A...
How cells adapt to varying environmental conditions is largely unknown. Here, we show that, in budding yeast, the RNA-binding and stress granule protein Pub1 has an intrinsic property form condensates upon starvation or heat that condensate formation associated with cell-cycle arrest. Release from arrest coincides dissolution, which takes minutes (starvation) hours (heat shock). In vitro reconstitution reveals different dissolution rates of starvation- heat-induced are due their material...
Amyotrophic lateral sclerosis (ALS) is a lethal disease characterized by motor neuron degeneration and associated with aggregation of nuclear RNA-binding proteins (RBPs), including FUS. How FUS neurodegeneration are prevented in healthy neurons remain critically unanswered questions. Here, we use combination ALS patient autopsy tissue induced pluripotent stem cell-derived to study the effects mutations on RBP homeostasis. We show that FUS' tendency aggregate normally buffered interacting...
Quantitative measurements of physical parameters become increasingly important for understanding biological processes. Brillouin microscopy (BM) has recently emerged as one technique providing the 3D distribution viscoelastic properties inside samples − so far relying on implicit assumption that refractive index (RI) and density can be neglected. Here, we present a novel method (FOB microscopy) combining BM with optical diffraction tomography epifluorescence imaging explicitly measuring...
Chromosome territories (CTs) in higher eukaryotes occupy tissue-specific non-random three-dimensional positions the interphase nucleus. To understand mechanisms underlying CT organization, we mapped position and transcriptional changes undifferentiated embryonic stem (ES) cells, during early onset of mouse ES cell differentiation terminally differentiated NIH3T3 cells. We found chromosome intermingling volume to be a reliable surface property, which can used define organization. Our results...
Abstract Remodeling of the local chromatin structure is essential for regulation gene expression. While a number biochemical and bioimaging experiments suggest decondensed structures are associated with transcription, direct visualization DNA transcriptionally active RNA polymerase II (RNA pol II) at super-resolution still lacking. Here we investigate isolated from HeLa cells using binding activatable localization microscopy (BALM). The sample preparation method preserved structural...
Genomes are spatially assembled into chromosome territories (CT) within the nucleus of living cells. Recent evidences have suggested associations between three-dimensional organization CTs and active gene clusters neighboring CTs. These part signaling networks sharing similar transcription factor or other downstream machineries. Hence, presence such in a cell type may regulate spatial chromosomes nucleus. However, given probabilistic nature positions complex (TFNs), quantitative methods to...
Transcription in higher organisms requires spatiotemporal coordination of transcription machinery and the factors at promoter sites. Toward this, recent evidence suggests that both static compartmentalization dynamic self-organization transcriptional apparatus are effect sites transcription. Although dynamics is essential to genome regulation, principles underlying this organization its functional coupling nuclear architecture unclear. In a study we revealed Uridine-5′-triphosphate (UTP)...
Abstract Quantitative measurements of physical parameters become increasingly important for understanding biological processes. Brillouin microscopy (BM) has recently emerged as one technique providing the 3D distribution viscoelastic properties inside samples — so far relying on implicit assumption that refractive index (RI) and density can be neglected. Here, we present a novel method (FOB microscopy) combining BM with optical diffraction tomography epi-fluorescence imaging explicitly...
Fused in sarcoma (FUS) is an abundant RNA-binding protein, which drives phase separation of cellular condensates and plays multiple roles RNA regulation. The ability FUS protein crucial to its function. Here, our molecular simulation study on the FUS-RNA complex provides atomic resolution insights into observations from biochemical studies also illuminates understanding driving forces that mediate structure, stability, interaction recognition motif (RRM) RGG domains with a stem-loop junction...