A5000118234- Sphingolipid Metabolism and Signaling
- Neurogenetic and Muscular Disorders Research
- Amyotrophic Lateral Sclerosis Research
- Extracellular vesicles in disease
- Lipid Membrane Structure and Behavior
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Drug Delivery Systems
- Endoplasmic Reticulum Stress and Disease
- Retinal Development and Disorders
- Cellular transport and secretion
- Alzheimer's disease research and treatments
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Research on Leishmaniasis Studies
- RNA regulation and disease
- Nanoparticle-Based Drug Delivery
- PARP inhibition in cancer therapy
- Insect and Pesticide Research
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- Advancements in Transdermal Drug Delivery
- Ion channel regulation and function
- Autoimmune Bullous Skin Diseases
- Essential Oils and Antimicrobial Activity
- Trypanosoma species research and implications
University of Kentucky
2019-2024
RWTH Aachen University
2019-2023
European Graduate School of Neuroscience
2020-2023
Universitätsklinikum Aachen
2021-2023
Maastricht University
2023
Weatherford College
2022
Leoni (Germany)
2022
Veterans Health Administration
2022
Central Drug Research Institute
2011-2017
Academy of Scientific and Innovative Research
2016-2017
The ethanol, methanol and acetone extracts of Aloe vera gel were studied for their antimicrobial activity against four Gram-positive Gram-negative bacteria using agar well diffusion method. showed varied levels the tested pathogens. ethanol higher while extract, least or no most Fractions obtained from by Thin Layer Column Chromatography antagonistic properties Spot Assay Technique. Compounds with maximum antibacterial isolated identified as p - coumaric acid (Mol. wt.165), ascorbic wt.177...
Amyotrophic lateral sclerosis (ALS) is a lethal disease characterized by motor neuron degeneration and associated with aggregation of nuclear RNA-binding proteins (RBPs), including FUS. How FUS neurodegeneration are prevented in healthy neurons remain critically unanswered questions. Here, we use combination ALS patient autopsy tissue induced pluripotent stem cell-derived to study the effects mutations on RBP homeostasis. We show that FUS' tendency aggregate normally buffered interacting...
Article19 March 2021Open Access Source DataTransparent process Hsp90-mediated regulation of DYRK3 couples stress granule disassembly and growth via mTORC1 signaling Laura Mediani Department Biomedical, Metabolic Neural Sciences, Centre for Neuroscience Nanotechnology, University Modena Reggio Emilia, Modena, Italy Search more papers by this author Francesco Antoniani ItalyThese authors are contributed equally to work as second, third Veronica Galli Jonathan Vinet Genomic Post-Genomic Center,...
The formation of extracellular vesicles (EVs) is induced by the sphingolipid ceramide. How this pathway regulated not entirely understood. Here, we report that ceramide transport protein (CERT) mediates a non-vesicular between endoplasmic reticulum (ER) and multivesicular endosome at contact sites. process depends on interaction CERT's PH domain with PI4P generated PI4KIIα endosomes. Furthermore, complex formed START CERT, which carries ceramide, Tsg101 protein, part endosomal sorting...
Abstract Amyloid-β (Aβ) associates with extracellular vesicles termed exosomes. It is not clear whether and how exosomes modulate Aβ neurotoxicity in Alzheimer’s disease (AD). We show here that brain tissue serum from the transgenic mouse model of familial AD (5xFAD) patients contains ceramide-enriched astrocyte-derived (termed astrosomes) are associated Aβ. In Neuro-2a cells, primary cultured neurons, human induced pluripotent stem cell-derived Aβ-associated astrosomes 5xFAD mice patient...
Previously, we showed that the sphingosine-1-phosphate (S1P) transporter spinster 2 (Spns2) mediates activation of microglia in response to amyloid β peptide (Aβ). Here, investigated if Ponesimod, a functional S1P receptor 1 (S1PR1) antagonist, prevents Aβ-induced glial cells and Alzheimer's disease (AD) pathology.
Abstract In Alzheimer’s disease (AD), reactive astrocytes produce extracellular vesicles (EVs) that affect mitochondria in neurons. Here, we show Aβ-induced generation of the sphingolipid ceramide by acid sphingomyelinase (A-SMase) triggered proinflammatory cytokine (C1q, TNF-α, IL-1α) release microglia, which induced phenotype and secretion EVs enriched with ceramide. These impeded capacity neurons to respond energy demand. Inhibition A-SMase Arc39 Imipramine reduced cytokines from...
Abstract Extracellular vesicles (EVs) are shed from the plasma membrane, but regulation and function of these EVs remain unclear. We found that oxidative stress induced by H 2 O in Hela cells stimulated filopodia formation secretion EVs. were small (150 nm) labeled for CD44, indicating they derived filopodia. Filopodia‐derived (sEVs) enriched with sphingolipid ceramide, consistent increased ceramide membrane Ceramide was colocalized neutral sphingomyelinase (nSMase2) acid (ASM), two...
Abstract ER stress signaling is linked to the pathophysiological and clinical disease manifestations in amyotrophic lateral sclerosis (ALS). Here, we have investigated stress-induced adaptive mechanisms C9ORF72 -ALS/FTD, focusing on uncovering early endogenous neuroprotective crosstalk between pathological responses onset progression. We provide evidence for of stress-mediated response patient-derived motoneurons (MNs), reflected by elevated increase GRP75 expression. These transiently...
Matrix type transdermal patches containing Glibenclamide were prepared using three different polymers by solvent evaporation technique. Aluminium foil cup method was used as a substrate. Polyethylene glycol (PEG) 400 plasticizer and Dimethyl sulfoxide (DMSO) penetration enhancer. The physicochemical parameters like weight variation, thickness, folding endurance, drug content, % moisture absorption loss evaluated. In vitro release studies skin permeation carried out Franz diffusion cell....
Conventional synthetic insecticides have limited success due to insect resistance and negative effects on off-target biota the environment. Although RNA interference (RNAi) is a tool that becoming more widely utilized in pest control products, naked dsRNA has most taxa. Nanocarriers shown promising results enhancing efficacy of this tool. In study, we used layer-by-layer electrostatic assembly where synthesized poly(acrylic acid) (PAA)-coated hydroxyapatite (HA) nanoparticles (PAA-HA NPs) as...
The aim of this study was to fabricate docetaxel loaded nanocapsules (DTX-NCs) with a high payload using Layer-by-Layer (LbL) technique by successive coating alternate layers oppositely charged polyelectrolytes. Developed (NCs) were characterized in terms morphology, particle size distribution, zeta potential (ζ-potential), entrapment efficiency and vitro release. morphological characteristics the NCs assessed transmission electron microscopy (TEM) that revealed polyelectrolytes around...
Mechanism of targeting MPs and absorption through Peyer’s patches, to lymphatics where an adult worm resides.
Granulovacuolar degeneration (GVD) occurs in Alzheimer's disease (AD) brain due to compromised autophagy. Endoplasmic reticulum (ER) function and RNA binding protein (RBP) homeostasis regulate We observed that the ER chaperones Glucose - regulated protein, 78 KDa (GRP78/BiP), Sigma receptor 1 (SigR1), Vesicle-associated membrane associated B (VAPB) were elevated many AD patients' subicular neurons. However, those neurons which affected by GVD showed lower chaperone levels, there was only...
A point mutation (P56S) in the gene-encoding vesicle-associated membrane-protein-associated protein B (VAPB) leads to an autosomal-dominant form of amyotrophic lateral sclerosis (ALS), classified as ALS-8. The mutant VAPB is characterized by ER-associated aggregates that lead a complete reorganization ER structures. Growing evidences suggest involvement ALS pathomechanisms. In fact, numerous studies demonstrated alteration also sporadic (sALS) and showed presence its when others ALS-related...
Mutations in RNA binding proteins (RBPs) and genes regulating autophagy are frequent causes of familial amyotrophic lateral sclerosis (fALS). The P56S mutation vesicle-associated membrane protein-associated protein B (VAPB) leads to fALS (ALS8) spinal muscular atrophy (SMA). While VAPB is primarily involved the unfolded response (UPR), vesicular trafficking initial steps pathway, effect mutant P56S-VAPB on regulation connection with RBP homeostasis has not been explored yet. Examining muscle...
Abstract Repeat expansions in the C9orf72 gene are a common cause of amyotrophic lateral sclerosis and frontotemporal lobar degeneration, two devastating neurodegenerative disorders. One proposed mechanisms GGGGCC repeat expansion is their translation into non-canonical dipeptide repeats, which can then accumulate as aggregates contribute to these pathologies. There five different proteins (polyGA, polyGR, polyPR, polyPA polyGP), some known be neurotoxic. In present study, we used BioID2...
The present study was focused on the development of surface modified gelatin nanoparticles (SGNPs) using novel ligand 4-sulfated N-acetyl galactosamine (4-SO4GalNAc) for specific targeting to macrophages. has been with potential moiety 4-SO4GalNAc, which further used preparation nanoparticles. have prepared by two step desolvation method. SGNPs and unmodified (GNPs) were loaded doxorubicin (DxR) its compared. Developed DxR-loaded (DxR-SGNPs) found negative zeta (−19.8 ± 0.22 mV) whereas GNPs...