A5031313062- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Helicobacter pylori-related gastroenterology studies
- Monoclonal and Polyclonal Antibodies Research
- Bacterial Infections and Vaccines
- Transgenic Plants and Applications
- Allergic Rhinitis and Sensitization
- Dermatology and Skin Diseases
- Galectins and Cancer Biology
- Esophageal and GI Pathology
- Chemokine receptors and signaling
- Toxin Mechanisms and Immunotoxins
- Immunodeficiency and Autoimmune Disorders
- Immune Response and Inflammation
- Gastric Cancer Management and Outcomes
- Gallbladder and Bile Duct Disorders
- Asthma and respiratory diseases
- Cancer Immunotherapy and Biomarkers
- Pancreatic and Hepatic Oncology Research
- Escherichia coli research studies
- T-cell and Retrovirus Studies
- Pharmaceutical Practices and Patient Outcomes
- Chromosomal and Genetic Variations
University of Sassari
2016-2025
Azienda Ospedaliera Universitaria Senese
2023
Policlinico S.Orsola-Malpighi
2023
Ospedale San Paolo
2023
IRCCS Ospedale San Raffaele
2023
Azienda Ospedaliera Citta' della Salute e della Scienza di Torino
2023
Azienda Ospedaliero-Universitaria Careggi
2005-2021
Society of Interventional Radiology
2021
Azienda Ospedaliero Universitaria di Sassari
2018-2019
University of Florence
1995-2004
The effects exerted on the in vitro development of antigen-specific T cell lines and clones by addition or neutralization interleukin 12 (IL-12) lymphocyte bulk culture were examined. specific for Dermatophagoides pteronyssinus group I (Der p I) derived presence IL-12 exhibited reduced ability to produce IL-4 increased interferon gamma (IFN-gamma), developed into Der I-specific CD4+ showing a helper type 0 (Th0)- Th1-, instead Th2-, like cytokine profile. In contrast, purified protein...
The effect of progesterone (P) on the cytokine production profile Ag-specific human CD4+ T cell lines and clones was investigated. specific for purified protein derivative or streptokinase (SK) derived in presence P exhibited significant increased ability to produce IL-5 comparison with absence P. Moreover, IL-4 significantly SK-specific this hormone. In addition, generated developed into showing a Th0-, instead Th1-like, profile. Furthermore, an established Th1 secretion did express mRNA...
The effects exerted on the in vitro development of purified protein derivative (PPD)-specific or Dermatophagoides pteronyssinus group I (Der p I)-specific T cell lines (TCL) and clones (TCC) by IL-4 IFN-gamma addition neutralization human PBMC cultures were examined. from two normal individuals, which stimulated with PPD then cultured IL-2 alone, developed into PPD-specific TCL TCC able to produce but not IL-5 (Th1-like). anti-IL-4 antibody bulk before cloning did influence profile cytokine...
Both interferon γ (IFN-γ) produced by T helper 1 (T H 1) lymphocytes and interleukin-4 (IL-4) 2 were reduced in either bulk circulating mononuclear cells or mitogen-induced CD4 + cell clones from the peripheral blood of individuals infected with human immunodeficiency virus (HIV). There was a preferential reduction producing IL-4 IL-5 advanced phases infection. However, enhanced proportions both 1-type 2-type cytokines 0 clones) generated skin-infiltrating that had been activated vivo...
Interleukin 12 (IL-12) facilitates the generation of a T helper type 1 (Th1) response, with high interferon gamma (IFN-gamma) production, while inhibiting IL-4-producing Th2 cells in polyclonal cultures both human and murine vivo mouse. In this study, we analyzed effect IL-12, present during cloning cells, on cytokine profile clones. The culture system used allows growth clones from virtually every cell, thus excludes possibility that selection precommitted Th cell precursors plays role...
The cytolytic potential of a total number 118 CD4+ human T cell clones specific for purified protein derivative (PPD) from Mycobacterium tuberculosis, tetanus toxoid, Lolium perenne group I allergen (Lol p I), Poa pratensis IX (Poa IX), or Toxocara canis excretory/secretory antigen(s) (TES) was assessed by both lectin (PHA)-dependent and MHC-restricted lytic assay compared with their profile cytokine secretion. majority Th1 Th0 exhibited activity in assays, whereas Th2 usually did not. There...
Phenotypic markers, localization, functional activities, and mechanisms of action in vitro CD4(+)CD25(+) T cells, purified from postnatal human thymuses, were investigated. These cells showed poor or no proliferation mixed lymphocyte culture (MLC), suppressed a dose-dependent fashion the proliferative response to allogeneic stimulation CD4(+)CD25(-) thymocytes. Virtually all thymocytes constitutively expressed cytoplasmic antigen (CTLA)-4, surface tumor necrosis factor type 2 receptor...
Immunization with chemically detoxified pertussis toxin can prevent severe whooping cough an efficacy similar to that of the cellular vaccine, which normally gives unwanted side effects. To avoid reversion toxicity and loss immunogenicity may follow chemical treatment toxin, inactive toxins were constructed by genetic manipulation. A number genetically engineered alleles genes, replacing either one or two key amino acids within enzymatically active S1 subunit, introduced into chromosome...
We analyzed at clonal level the functional profile of circulating or skin-infiltrating T lymphocytes from two individuals infected with human immunodeficiency virus type 1 (HIV-1), suffering a Job's-like syndrome (eczematous dermatitis, recurrent skin and sinopulmonary infections, hypergammaglobulinemia E) showing virtually no CD4+ cells. Most CD3+ cell clones generated both patients were CD4- CD8+ TCR alpha beta +. The others CD8- + which exhibited reduced mRNA expression for CD8 molecule...
Cells expressing the chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) and chemokine C receptor (CCR)4 were consistently detected in circulation of healthy subjects, whereas numbers CCR3 much lower. While all CCR4+ T cells, a small proportion CRTH2+, about half few CCR3+ basophils. Only CRTH2+ contained or Tc2 but neither Th0 Tc0, nor Th1 Tc1 although not them produced Th2-type cytokines. By contrast, both Tc0 even clearly classifiable for their cytokine profile....
Activation of Th1 lymphocytes, IFN-γ production and macrophage activation are crucial in defense against Mycobacteria. In developing countries, Th2 IL-4 have been associated vitro with tuberculosis poor clinical outcome after treatment. Serological markers [soluble lymphocyte gene (LAG)-3] (IgE, solubleCD30, CCL22/macrophage-derived chemokine) activity were measured 414 HIV-negative patients from The Gambia Guinée healthy household community controls. Measurements repeated during treatment...
The protein toxin VacA, produced by cytotoxic strains of Helicobacter pylori, causes a vacuolar degeneration cells, which eventually die. VacA is strongly activated short exposure to acidic solutions in the pH 1.5-5.5 range, followed neutralization. Activated has different CD and fluorescence spectra limited proteolysis fragmentation pattern from kept at neutral pH. Moreover, resistant 1.5 pepsin. relevance these findings pathogenesis H. pylori-induced gastrointestinal ulcers discussed.
ABSTRACT Chlamydia pneumoniae , a human pathogen causing respiratory infections and probably contributing to the development of atherosclerosis heart disease, is an obligate intracellular parasite which for replication needs productively interact with enter cells. Because intrinsic difficulty in working C. absence reliable tools its genetic manipulation, molecular definition chlamydial cell surface still limited, thus leaving mechanisms entry largely unknown. In effort define protein...
Disease-associated strains of Helicobacter pylori produce a potent toxin that is believed to play key role in peptic ulcer disease man. In vitro the causes severe vacuolar degeneration target cells and has thus been termed VacA (for vacuolating cytotoxin A). Cytotoxic activity associated with > 600-kD protein consisting several copies 95-kD polypeptide undergoes specific proteolytic cleavage after release from bacteria 37- 58-kD fragments. Quick freeze, deep etch electron microscopy...
Proposed co-factors triggering the pathogenesis of multiple sclerosis (MS) are Epstein Barr virus (EBV), and potentially neuropathogenic MSRV (MS-associated retrovirus) syncytin-1, W family human endogenous retroviruses.In search links, expression HERV-W/MSRV/syncytin-1, with/without exposure to EBV or glycoprotein350 (EBVgp350), was studied on peripheral blood mononuclear cells (PBMC) from healthy volunteers MS patients, astrocytes, by discriminatory env-specific RT-PCR assays, flow...
Ultrasonography is a fundamental diagnostic imaging tool in everyday clinical practice. Here, we are unique describing the use of functionalized multiwalled carbon nanotubes (MWCNTs) as hyperechogenic material, suggesting their potential application ultrasound contrast agents. Initially, carried out thorough investigation to assess echogenic property vitro. We demonstrated long-lasting properties. also showed that signal MWCNTs higher than graphene oxide, pristine MWCNTs, and single-walled...
Graphene oxide (GO) is attracting great interest in biomedical sciences. The impact of GO on immune cells one fundamental area study that often overlooked, but critical terms clinical translation. This work investigates the effects two types thoroughly characterized sheets, different their lateral dimension, human peripheral provided from healthy donors using a wide range assays. After evaluation cell viability, gene expression was analyzed, following exposure 84 genes related to innate and...
Abstract The preferential association of some chemokine receptors with human Th1 or Th2 cells has recently been reported. In this study, the expression CCR3, CCR5, CXCR3, and CXCR4 were analyzed by flow cytometry in three distinct vitro models Th1/Th2 polarization, activated naive memory T cells, T-cell clones, which intracellular synthesis interferon-γ (IFN-γ) interleukin-4 (IL-4) surface CD30 LAG-3 also assessed. Moreover, using immunohistochemistry vivo was examined gut patients suffering...
Umbelical cord blood lymphocytes showed consistent proliferation in response to Dermatophagoides group I (Der p I) and occasionally even Lolium (Lol allergen. These data suggest sensitization utero of T cells due inhalation these allergens by the mother during pregnancy.