A5027310116- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Allergic Rhinitis and Sensitization
- Receptor Mechanisms and Signaling
- Bipolar Disorder and Treatment
- Neurotransmitter Receptor Influence on Behavior
- Food Allergy and Anaphylaxis Research
- Asthma and respiratory diseases
- Attention Deficit Hyperactivity Disorder
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Contact Dermatitis and Allergies
- T-cell and Retrovirus Studies
- Urticaria and Related Conditions
- Immune Response and Inflammation
- Dermatology and Skin Diseases
- Pancreatic function and diabetes
- Animal Disease Management and Epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Pharmacological Effects and Toxicity Studies
- Schizophrenia research and treatment
- Immunotherapy and Immune Responses
- Occupational exposure and asthma
- Eosinophilic Esophagitis
University of Pisa
1996-2025
University of Florence
1992-2024
University of Udine
2003-2023
Presidio Ospedaliero
2020-2022
Ospedale Santa Maria della Misericordia di Udine
2004-2020
Azienda Ospedaliera S.Maria
2003-2013
Politecnico di Milano
2012
University of Padua
1986-2008
Azienda Ospedaliero-Universitaria Careggi
1999
Inserm
1997
Abstract IL-10 gene transcription and protein production was assessed in both type 1 (Th1) 2 (Th2) CD4+ human T cell clones by polymerase chain reaction ELISA, respectively. Although Th2 apparently showed higher mRNA levels, expression consistently found Th1 clones, as well. Likewise, measurable levels were the supernatants of clones. The effect (h-IL-10) viral (v-IL-10) on proliferative response cytokine also investigated. Addition culture h-IL-10 v-IL-10 significantly reduced proliferation...
A large series of T cell clones (TCC) specific for purified protein derivative (PPD) Mycobacterium tuberculosis (total 60) or Toxocara canis excretory/secretory (TES) antigen 69) were established from the peripheral blood two healthy individuals and analyzed their profile cytokine production in response to stimulation with either polyclonal activator phorbol myristate acetate plus anti-CD3 antibody. Under both these experimental conditions, great majority PPD-specific TCC secreted IL-2...
The effects exerted on the in vitro development of purified protein derivative (PPD)-specific or Dermatophagoides pteronyssinus group I (Der p I)-specific T cell lines (TCL) and clones (TCC) by IL-4 IFN-gamma addition neutralization human PBMC cultures were examined. from two normal individuals, which stimulated with PPD then cultured IL-2 alone, developed into PPD-specific TCL TCC able to produce but not IL-5 (Th1-like). anti-IL-4 antibody bulk before cloning did influence profile cytokine...
Chronic antral gastritis following Helicobacter pylori (Hp) infection is characterized by a cellular inflammatory infiltrate whose cytokines may represent host-dependent factor influencing the outcome of infection. The pattern produced immunologically active cells in gastric antrum was analyzed at mRNA level biopsies from five Hp-infected patients with duodenal ulcer and three Hp-negative dyspeptic controls. T cell clones were generated parallel same assessed for reactivity to Hp Ags,...
Both interferon γ (IFN-γ) produced by T helper 1 (T H 1) lymphocytes and interleukin-4 (IL-4) 2 were reduced in either bulk circulating mononuclear cells or mitogen-induced CD4 + cell clones from the peripheral blood of individuals infected with human immunodeficiency virus (HIV). There was a preferential reduction producing IL-4 IL-5 advanced phases infection. However, enhanced proportions both 1-type 2-type cytokines 0 clones) generated skin-infiltrating that had been activated vivo...
The cytolytic potential of a total number 118 CD4+ human T cell clones specific for purified protein derivative (PPD) from Mycobacterium tuberculosis, tetanus toxoid, Lolium perenne group I allergen (Lol p I), Poa pratensis IX (Poa IX), or Toxocara canis excretory/secretory antigen(s) (TES) was assessed by both lectin (PHA)-dependent and MHC-restricted lytic assay compared with their profile cytokine secretion. majority Th1 Th0 exhibited activity in assays, whereas Th2 usually did not. There...
A large panel of human CD4+ T helper (Th) cell clones with established Th1, Th2, or Th0 profiles cytokine secretion were examined for the expression CD30, a member tumor necrosis factor receptor superfamily. Th1 expressed poor no CD30 mRNA, and showed low undetectable both membrane soluble (sCD30) protein, whereas Th2 mRNA released substantial amounts sCD30. exhibited an intermediate pattern release. When cells from same donor stimulated three different antigens (purified protein derivative,...
Abstract Biopsy specimens were obtained from the bronchial or nasal mucosa of three patients with grass pollen‐induced asthma rhinitis 48 h after positive provocation test pollen extract. T cell clones (TCC), derived these and control specimens, then assessed for their phenotype, allergen‐specificity, profile cytokine secretion ability to provide B help IgE synthesis. Out 50 61 CD4 + TCC two patient atopic 11 (22%) 19 (31%), respectively, showed both proliferation production in response...
Abstract A total number of 132 T cell clones (TCC) were obtained by PHA-stimulation single cells from mononuclear suspensions conjunctival flogistic infiltrates three patients with vernal conjunctivitis (VC). The phenotype and functional properties these TCC compared those 122 contemporarily established PB the same patients, 120 lymph nodes nonspecific hyperplastic lymphoadenitis 159 thyroid lymphocyte Graves' disease. great majority displayed CD4+ CD8- (CD4/CD8 ratios ranging 6.1 to 7.0),...
A large number of CD4+ human T helper type 1 (Th1) clones specific for purified protein derivative and Th2 the excretory/secretory antigen Toxocara canis, derived from same individuals, were analyzed both cytotoxic capacity function immunoglobulin (Ig) synthesis. The great majority Th1, but only a minority exhibited cytolytic activity. All (noncytolytic) induced IgM, IgG, IgA, IgE synthesis by autologous B cells in presence antigen, degree response was proportional to added cells. Under...
Studies of the prevalence different types food allergy in adults are lacking.To define IgE-mediated allergies Italian attending clinics and to assess possible differences associated with geographical position and/or dietary habits.Seventeen outpatient scattered throughout Italy participated a multi-centre study 2007. The number atopic subjects allergic patients along clinical features were recorded by pre-defined criteria. Patients unequivocal history confirmed positive skin prick test...
Data about food-induced anaphylaxis in Italy are missing.It was the aim of this study to detect main foods/food allergens causing Italy.The frequency and relative importance many offending foods were assessed 1,110 adult patients with food allergy diagnosed by common criteria at 19 centres scattered throughout from 1 January 31 December 2007.Fifty-eight (5%) food-allergic experienced least episode anaphylaxis. On average, they older than other (34 vs. years; p < 0.05). The majority...
We have recently shown that CD30, a member of the tumor necrosis factor/nerve growth factor receptor superfamily, is preferentially expressed by human T cell clones producing helper (Th) type 2 cytokines. report here costimulation with an agonistic anti-CD30 monoclonal antibody enhanced antigen (Ag)-induced proliferation and cytokine secretion established Th2 Th0 clones. Moreover, peripheral blood mononuclear cells same resulted in preferential development Ag-specific lines showing Th2-like...
Lymphocytic infiltration of muscular and connective tissues the retroorbital (RO) space is a histological hallmark Graves' ophthalmopathy (GO). We have characterized some phenotypical functional features T cells derived from RO infiltrates four GO patients who were submitted to orbital decompression. Fragments tissue cultured for 7 days in IL-2-conditioned medium order generate cell lines vivo activated cells. Phenotypical analysis freshly isolated peripheral blood (PB) lymphocytes both...
Circulating T cells from four patients with the hyper-IgE syndrome were found to produce significantly lower concentrations of interferon-gamma (IFN-gamma) in response stimulation phytohemagglutinin (PHA) than did eight age-matched healthy controls, three atopic dermatitis and one patient chronic granulomatous disease. A clonal analysis revealed that had markedly proportions circulating able IFN-gamma tumor necrosis factor-alpha (TNF-alpha) comparison controls. In contrast, peripheral blood...
Psychiatric disorders often require pharmacological interventions to alleviate symptoms and improve quality of life. However, achieving an optimal therapeutic outcome is challenging due several factors, including variability in the individual response, inter-individual differences drug metabolism, interactions polytherapy. Therapeutic monitoring (TDM), by measuring concentrations biological samples, represents a valuable tool address these challenges, tailoring medication regimens each...
Muscarinic acetylcholine receptors are prototypical G protein-coupled (GPCRs), members of a large family 7 transmembrane mediating wide variety extracellular signals. We show here, in cultured cells and murine model, that the carboxyl terminal fragment muscarinic M2 receptor, comprising regions 6 (M2tail), is expressed by virtue an internal ribosome entry site localized third intracellular loop. Single-cell imaging import isolated yeast mitochondria reveals M2tail, whose expression...