A5011273738- Muscle metabolism and nutrition
- Adipose Tissue and Metabolism
- Cytokine Signaling Pathways and Interactions
- Autophagy in Disease and Therapy
- Metabolism, Diabetes, and Cancer
- Cancer, Hypoxia, and Metabolism
- Diet and metabolism studies
- Nutrition and Health in Aging
- Skin Protection and Aging
- Digestive system and related health
- Wound Healing and Treatments
- interferon and immune responses
- Neuroinflammation and Neurodegeneration Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Pressure Ulcer Prevention and Management
- Adipokines, Inflammation, and Metabolic Diseases
- Advanced Nanomaterials in Catalysis
- Muscle Physiology and Disorders
- Receptor Mechanisms and Signaling
- PI3K/AKT/mTOR signaling in cancer
- Mitochondrial Function and Pathology
- Antiplatelet Therapy and Cardiovascular Diseases
- Pancreatic function and diabetes
- Polyamine Metabolism and Applications
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
University of L'Aquila
2015-2024
Fondazione Vincenzo Pansadoro
2013
University of Parma
2007
Columbia University
2002
Cleveland Clinic
1995-1997
Muscarinic acetylcholine receptors are prototypical G protein-coupled (GPCRs), members of a large family 7 transmembrane mediating wide variety extracellular signals. We show here, in cultured cells and murine model, that the carboxyl terminal fragment muscarinic M2 receptor, comprising regions 6 (M2tail), is expressed by virtue an internal ribosome entry site localized third intracellular loop. Single-cell imaging import isolated yeast mitochondria reveals M2tail, whose expression...
Members of the suppressor cytokine signaling (SOCS) family molecules regulate activation signaling. Experimental evidence indicates that SOCS expression is induced by cytokines and pro-inflammatory stimuli controlled at both transcriptional post-transcriptional levels. proteins are unstable seem to be rapidly degraded proteasomal pathways. However, mechanisms which protein levels regulated remain unclear. Here, we show TRIM8/GERP, a RING finger protein, interacts with SOCS-1 in vitro andin...
Different Stat proteins are activated through phosphorylation of unique tyrosine residues in response to different cytokines and growth factors. Interferon-gamma activates Stat1 molecules that form homodimers bind cognate DNA elements. Here we show treatment permeabilized cells with 200-500 microM peroxo-derivatives vanadium, molybdenum, tungsten results the accumulation constitutively phosphorylated alpha molecules. In contrast, orthovanadate, vanadyl sulfate, molybdate, tungstate at same...
Cancer cells require both energy and material to survive duplicate in a competitive environment. Nutrients, such as amino acids (AAs), are not only caloric source, but can also modulate cell metabolism modify hormone homeostasis. Our hypothesis is that the environmental messages provided by AAs rule dynamics of cancer life or death, alteration balance between essential (EAAs) non-essential (NEAAs) (lower higher than 50%, respectively) present nutrients may represent key instrument alter...
An adequate intake of essential (EAA) and non-essential amino acids (NEAA) is crucial to preserve cell integrity whole-body metabolism. EAA introduced with diet may be insufficient meet the organismal needs, especially under increased physiological requirements or in pathological conditions, condition lifespan. We therefore examined effects iso-caloric providing same nitrogenous content diets, any containing different stoichiometric blends EAA/NEAA, on mouse Three groups just-weaned male...
In this study, we investigated whether cerium oxide nanoparticles (CeO2-NPs), a promising antioxidant nanomaterial, may contrast retinal vascular alterations induced by oxidative damage in vitro and vivo. For the vivo experiments, light (LD) animal model of Age-Related Macular Degeneration (AMD) was used CeO2-NPs were intravitreally injected. significantly decreased endothelial growth factor (VEGF) protein levels, reduced neovascularization deep plexus, inhibited choroidal sprouting into...
Background Intestinal fibrosis is characterized by abnormal production and deposition of extracellular matrix (ECM) proteins activated myofibroblasts. The main progenitor cells myofibroblasts are the fibroblasts epithelial cells, latter through epithelial-mesenchymal transition (EMT). Aim To evaluate action new PPAR-γ modulator, GED-0507-34 Levo (GED) on expression EMT associated regulatory such as TGF-β, Smad3, E-cadherin, Snail, ZEB1, β-catenin, GSK-3β, in a mouse model DSS-induced...
Klotho proteins (α- and β) are membrane-based circulating that regulate cell metabolism, as well the lifespan modulating activity of Fibroblast Growth Factors (FGFs). Recent data has shown higher plasma levels reduce cardiovascular risk, suggesting a protective role in diseases. However, although so far it been identified various organs, is unknown whether cardiomyocytes express FGFs, high risk could affect cardiac expression Klotho, FGFs other molecules.We selected 20 patients with an...
Tebuconazole and Econazole are triazole imidazole fungicides currently used worldwide. Although their reproductive toxicity in mammals has been described, effect on male systems poorly investigated. As humans may be exposed to different azole compounds simultaneously, the combinational vitro of (MIX) mouse Sertoli TM4 cells was This study demonstrates that (40 µM) (20 act synergistically mediating decrease mitochondrial membrane potential (ΔΨm) changes morphology. These events were...
Objective Obesity is the result of white adipose tissue accumulation where excess food energy stored to form triglycerides. De novo lipogenesis (DNL) continuous process new fat production and driven by transcription factor ChREBP. During adipogenesis, adipocytes change their morphology entire cell volume occupied one large lipid droplet. Recent studies have implicated an essential role autophagy in adipogenic differentiation, cytoplasmic remodelling mitochondria reorganization. The phenolic...
Retinal pigment epithelium (RPE) dysfunction and degeneration underlie the development of age-related macular (AMD), which is leading cause blindness worldwide. In this study, we investigated whether cerium oxide nanoparticles (CeO2-NPs or nanoceria), are anti-oxidant agents with auto-regenerative properties, able to preserve RPE. On ARPE-19 cells, found that CeO2-NPs promoted cell viability against H2O2–induced cellular damage. For in vivo studies, used a rat model acute light damage (LD),...
Patients with metabolic syndrome (MetS) have an increased risk of cardiovascular disease. Data obtained from muscle biopsies demonstrated altered insulin signaling (IS) in patients MetS. The IS regulates critical cell functions including molecular-regulated cellular metabolite fluxes, protein and energetic metabolism, proliferation apoptosis consequent regulation life endothelial homeostasis blood coagulation. However, little is known about MetS patients. aim this study was to develop a...
Abstract The light damage (LD) model was mainly used to study some of the main aspects age related macular degeneration (AMD), such as oxidative stress and photoreceptor death. Several protocols light-induced retinal exist. Acute is characterized by a brief exposure (24 hours) high intensity (1000 lux) leads focal retina which progresses over time. To date there are not experimental data that relate this neovascular events. Therefore, purpose characterize after an acute assess whether...
Tumors escape from immune surveillance by, among other mechanisms, the down- regulation of endothelial adhesion molecules, such as ICAM-1, and by unresponsiveness to inflammatory signals, a process mediated angiogenic factors that is called cell anergy. Here we present biological these processes. The basic fibroblast growth factor (bFGF/FGF-2) was found inhibit tumor necrosis factor-alpha (TNF-alpha)- induced elevation at transcriptional level. Furthermore, bFGF inhibits TNF-mediated...