A5073735460- Mesenchymal stem cell research
- Prion Diseases and Protein Misfolding
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Air Quality and Health Impacts
- Mitochondrial Function and Pathology
- Cellular transport and secretion
- Glioma Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Alzheimer's disease research and treatments
- Microtubule and mitosis dynamics
- Neurological diseases and metabolism
- Cancer-related Molecular Pathways
- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
- Proteoglycans and glycosaminoglycans research
- Tissue Engineering and Regenerative Medicine
- Erythrocyte Function and Pathophysiology
- Pancreatic function and diabetes
- Caveolin-1 and cellular processes
- RNA regulation and disease
- Cell death mechanisms and regulation
- Nerve injury and regeneration
- Extracellular vesicles in disease
- Global Cancer Incidence and Screening
Link Campus University
2023-2025
IMT School for Advanced Studies Lucca
2019-2023
Sapienza University of Rome
2008-2022
University of L'Aquila
2002-2022
Sabin Vaccine Institute
2012-2021
Tecnologie Avanzate (Italy)
2021
Istituto Superiore di Sanità
2003-2005
ABSTRACT Despite inducing a strong host cellular and humoral immune response, the helminth Echinococcus granulosus is highly successful parasite that develops, progresses, ultimately causes chronic disease. Although surgery remains preferred therapeutic option, pharmacological research now envisages antihelminthic strategies. To understand mechanisms E. uses to escape immunosurveillance promote infection, we investigated how two hydatid cyst components, purified antigen B (AgB) sheep fluid...
Abstract Intercellular mitochondrial transfer (MT) is a newly discovered form of cell-to-cell signalling involving the active incorporation healthy mitochondria into stressed/injured recipient cells, contributing to restoration bioenergetic profile and cell viability, reduction inflammatory processes normalisation calcium dynamics. Recent evidence has shown that MT can occur through multiple cellular structures mechanisms: tunneling nanotubes (TNTs), via gap junctions (GJs), mediated by...
In this study we analyzed the interaction of prion protein PrP C with components glycosphingolipid‐enriched microdomains in lymphoblastoid T cells. was distributed small clusters on plasma membrane, as revealed by immunoelectron microscopy. is present microdomains, since it coimmunoprecipitates GM3 and raft marker GM1. A strict association between Fyn scanning confocal microscopy coimmunoprecipitation experiments. The phosphorylation ZAP‐70 immunoprecipitated anti‐PrP after cell activation....
The highly conserved Golgi phosphoprotein 3 (GOLPH3) protein has been described as a Phosphatidylinositol 4-phosphate [PI(4)P] effector at the Golgi. GOLPH3 is also known potent oncogene, commonly amplified in several human tumors. However, molecular pathways through which oncoprotein acts malignant transformation are largely unknown. never involved cytokinesis. Here, we characterize Drosophila melanogaster homologue of during cell division. We show that accumulates cleavage furrow and...
Over recent years, many authors discussed the effects of different natural compounds on glioblastoma (GBM). Due to its capacity impair survival and progression cancer types, saffron extract (SE), named crocetin (CCT), is particularly noteworthy. In this work, we elucidated antitumor properties in glioma vivo vitro models for first time. The results showed that four tumor cell lines observed study (U251, U87, U138, U373), which were treated with increasing doses crocetin, antiproliferative...
Therapies against glioblastoma (GBM) show a high percentage of failure associated with the survival glioma stem cells (GSCs) that repopulate treated tumours. Forced differentiation GSCs is promising new approach in cancer treatment. Erythropoietin-producing hepatocellular (Eph) receptors drive tumourigenicity and stemness GBM. We tested GLPG1790, first small molecule inhibition activity versus inhibitor various Eph receptor kinases, preclinical GBM models using vitro vivo assays. GLPG1790...
Methylglyoxal (MG) is a potent precursor of glycative stress (abnormal accumulation advanced glycation end products, AGEs), relevant condition underpinning the etiology several diseases, including those oral cave. At present, synthetic agents able to trap MG are known; however, they have never been approved for clinical use because their severe side effects. Hence, search bioactive natural scavengers remains sector strong research interest. Here, we investigated whether and how oleuropein...
As previously described by several authors, dental pulp stem cells (DPSCs), when adequately stimulated, may acquire a neuronal-like phenotype acting as favorable source of in the generation nerves. Besides, it is known that hypoxia conditioning capable stimulating cell differentiation well survival and self-renewal, multiple growth factors, including Epidermal Growth factor (EGF) basic fibroblast (bFGF), are often involved induction neuronal progenitor cells. In this work, we investigated...
In this study, we investigated whether cerium oxide nanoparticles (CeO2-NPs), a promising antioxidant nanomaterial, may contrast retinal vascular alterations induced by oxidative damage in vitro and vivo. For the vivo experiments, light (LD) animal model of Age-Related Macular Degeneration (AMD) was used CeO2-NPs were intravitreally injected. significantly decreased endothelial growth factor (VEGF) protein levels, reduced neovascularization deep plexus, inhibited choroidal sprouting into...
Dental pulp has been revealed as an accessible and a rich source of mesenchymal stem cells (MSCs) its biological potential is currently under intense investigation. MSCs from dental (DPSCs) have indicated heterogeneous population oriented not only in repairing dentine but also maintaining vascular nervous homeostasis the teeth. We sought to verify phenotype isolated young donors investigate vitro their role pericyte-like cells. Specifically, we evaluated how culture conditions can modulate...
Cell proliferation requires the orchestrated actions of a myriad proteins regulating DNA replication, repair and damage tolerance, cell cycle. Proliferating nuclear antigen (PCNA) is master regulator which interacts with multiple functioning in these processes, this makes PCNA an attractive target anticancer therapies. Here, we show that cell-penetrating peptide containing AlkB homolog 2 PCNA-interacting motif (APIM), ATX-101, has antitumor activity panel human glioblastoma multiforme (GBM)...
Aims/Purpose: Age‐related macular degeneration (AMD) is the leading cause of blindness in elderly. To date, there are no effective therapies to counteract AMD towards most severe stages characterized by a progressive loss photoreceptors triggered retinal pigmented epithelium dysfunction. Given their easily source and high proliferative potential, Dental Pulp Stem Cells (DPSCs) showed promise for regenerative medicine. The main advantage DPSCs related immunosuppressive immunoregulatory...
In this investigation we show that the death-inducing signaling complex (DISC) associates with glycosphingolipid-enriched microdomains (GEM) upon CD95/Fas engagement. We primarily analyzed ganglioside pattern and composition of GEM after triggering through observed GM3 is main constituent GEM. Stimulation anti-CD95/Fas did not cause translocation gangliosides within or from fraction. Scanning confocal microscopy showed induced a significant GM3-caspase-8 association, as revealed by nearly...
Cellular prion protein (PrPc) is a physiological constituent of eukaryotic cells. The cellular pathways underlying prions spread from the sites infection/peripheral replication to central nervous system are still not elucidated. Membrane-derived microvesicles (MVs) submicron (0.1–1 µm) particles, that released by cells during plasma membrane shedding processes. They usually liberated different cell types, mainly upon activation as well apoptosis, in this case, one their hallmarks exposure...
Human Dental Pulp Stem Cells (hDPSCs) represent a type of adult mesenchymal stem cells that have the ability to differentiate in vitro several lineages such as odontoblasts, osteoblasts, chondrocytes, adipocytes and neurons. In current work, we used hDPSCs experimental model study role recombinant prion protein 23–231 (recPrPC) neuronal differentiation process, signal pathway activation ERK 1/2 Akt. We demonstrated recPrPC was able activate an intracellular mediated by...