A5062678613- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- Glioma Diagnosis and Treatment
- MicroRNA in disease regulation
- Biomedical Text Mining and Ontologies
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- Protease and Inhibitor Mechanisms
- Cancer Research and Treatments
- Polymer Surface Interaction Studies
- Bone Metabolism and Diseases
- Neurogenetic and Muscular Disorders Research
- Amyotrophic Lateral Sclerosis Research
- Cell Adhesion Molecules Research
- interferon and immune responses
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Endoplasmic Reticulum Stress and Disease
- Polyamine Metabolism and Applications
- Peptidase Inhibition and Analysis
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Surface Modification and Superhydrophobicity
- Tissue Engineering and Regenerative Medicine
- RNA Interference and Gene Delivery
Jožef Stefan Institute
2017-2025
National Institute of Biology
2009-2018
University of Ljubljana
2005-2011
We have investigated the influence of various plasma treatments electrospun polycaprolactone (PCL) scaffolds on adhesion and proliferation human umbilical endothelial cells (HUVEC). The PCL were treated in plasmas created O 2 , NH 3 or SO gas at identical conditions. Surface functionalization plasma-treated samples was determined using X-ray photoelectron spectroscopy. Cell morphology by scanning electron microscopy treatment cell viability evaluated with assay (MTT assay). results showed...
// Neža Podergajs 1 , Helena Motaln Uroš Rajčević 2 Urška Verbovšek Marjan Koršič 3 Nina Obad 4 Heidi Espedal Miloš Vittori Christel Herold-Mende 5 Hrvoje Miletic Rolf Bjerkvig 4, 6 Tamara Lah Turnšek 1, 7 Department of Genetic Toxicology and Cancer Biology, National Institute 1000 Ljubljana, Slovenia Biochemistry, Blood Transfusion Centre Slovenia, Neurosurgery, University Medical Centre, Biomedicine, Bergen, 5009 Norway Division Neurosurgical...
Glioblastoma multiforme are an aggressive form of brain tumors that characterized by distinct invasion single glioblastoma cells, which infiltrate the parenchyma. This appears to be stimulated communication between cancer and stromal cells. Mesenchymal stem cells (MSCs) part microenvironment, their 'cross-talk' with is still poorly understood. Here, we examined effects bone marrow-derived MSCs on two different established cell lines U87 U373. We focused mutual direct MSC/glioblastoma contact...
The GGGGCC (G4C2) repeat expansion mutation in C9ORF72 gene is the most common genetic cause of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). Transcription formation nuclear RNA foci, which sequester specific RNA-binding proteins one possible pathological mechanisms. Here, we show that (G4C2)n predominantly associates with essential paraspeckle SFPQ, NONO, RBM14, FUS hnRNPH co-localizes known paraspeckle-associated hLinc-p21. As paraspeckles motor neurons has been...
Glioblastoma multiforme is the most lethal of brain cancer, and it comprises a heterogeneous mixture functionally distinct cancer cells that affect tumor progression. We examined U87, U251, U373 malignant cell lines as in vitro models to determine impact cellular cross-talk on their phenotypic alterations co-cultures. These were also studied at transcriptome level, define mechanisms observed mutually affected genomic stability, proliferation, invasion resistance temozolomide. This first...
Glioblastoma multiforme (GBM) displays high resistance to radiation and chemotherapy, due the presence of a fraction GBM stem-like cells (GSLCs), which are thus representing target for elimination. Since mesenchymal stem (MSCs) display tumor tropism, we examined possible antitumor effects secreted factors from human MSCs on four GSLC lines (NCH421k, NCH644, NIB26, NIB50). We found that conditioned media bone marrow umbilical cord-derived (MSC-CM) mediated cell cycle arrest GSLCs by...
Chitosan is a water-soluble polysaccharide with good adherence to negatively charged surfaces and reported antimicrobial anti-inflammatory properties. Coating the of medical devices chitosan promising strategy for harnessing these benefits. However, surface properties commercial polymers need be altered enable bonding thin films. In this study, adhesion onto plasma-treated polyvinyl chloride (PVC) metabolic activity urothelial cells on chitosan-coated medical-grade PVC used synthesis urinary...
In contrast to the application of human mesenchymal stem cells (hMSCs) in regenerative medicine, only a limited number studies are addressing their use anticancer therapy. As latter may represent new hope improve survival patients with glioblastoma multiformae (GBM), most common and malignant form brain tumors, we aimed investigate interactions hMSCs GBM under vitro conditions. Four hMSC clones three different cell lines were used study mutual paracrine cocultures compared monocultures,...
Cancer genome and transcriptome analyses advanced our understanding of cancer biology. We performed analysis all known genes peptidases also called proteases their endogenous inhibitors in glioblastoma multiforme (GBM), which is one the most aggressive deadly types brain cancers, where unbalanced proteolysis associated with tumor progression.Comparisons were between transcriptomics primary GBM tumors unmatched non-malignant tissue, cell lines (U87-MG U373) a control human astrocyte line...
Abstract Familial dysautonomia (FD) is a rare genetic disease with no treatment, caused by an intronic point mutation (c.2204+6T>C) that negatively affects the definition of exon 20 in elongator complex protein 1 gene (ELP1 also known as IKBKAP). This substitution modifies 5′ splice site and, combination regulatory splicing factors, induces different levels skipping, various tissues. Here, we evaluated therapeutic potential novel class U1 snRNA molecules, exon-specific U1s (ExSpeU1s),...
Glioblastoma multiforme (GBM) represents the most lethal brain tumour, and these tumours have very limited treatment options. Mesenchymal stem cells (MSC) are considered as candidates for advanced cell therapies, due to their tropism towards GBM, possibly affecting malignancy, thus also representing a potential therapeutic vector. Therefore, we aimed compare effects of bone-marrow-derived versus adipose-tissue-derived MSC (BM-/AT-MSC) on heterogeneous populations tumour cells. This cells'...
Background Glioblastoma multiforme (GBM) is among the most aggressive cancers with a poor prognosis in spite of plethora established diagnostic and prognostic biomarkers treatment modalities. Therefore, current goal detection novel biomarkers, possibly detectable blood GBM patients that may enable an early diagnosis are potential therapeutic targets, leading to more efficient interventions. Experimental Procedures MicroRNA profiling 734 human human-associated viral miRNAs was performed on...
Malignant gliomas are among the rarest brain tumours, and they have worst prognosis. Grade IV astrocytoma, known as glioblastoma multiforme (GBM), is a highly lethal disease where standard therapies of surgery, followed by radiation chemotherapy, cannot significantly prolong life expectancy patients. Tumour recurrence shows more aggressive form compared to primary tumour, results in patient survival from 12 15 months only. Although still controversial, cancer stem cell hypothesis postulates...
The most aggressive subtype of brain tumors is glioma WHO grade IV, the glioblastoma (GBM). present work aims to elucidate role kinin receptors in interactions between GBM cells and mesenchymal stem (MSC). cell line U87-MG was stably transfected express dsRed protein, single cloned, expanded, cultured with MSC, both direct co-cultures (DC) indirect (IC) at equal number ratio for 72 h. Up- down-regulation matrix metalloproteases (MMP)-9 expression MSC cells, respectively, co-culture points...