A5020802355- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- Marine Biology and Ecology Research
- Aquatic Invertebrate Ecology and Behavior
- Subterranean biodiversity and taxonomy
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Pathogens and Resistance
- Genetically Modified Organisms Research
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Statistical Distribution Estimation and Applications
- Crustacean biology and ecology
- Research Data Management Practices
- Plant and animal studies
- Advanced Statistical Methods and Models
- CRISPR and Genetic Engineering
- Protease and Inhibitor Mechanisms
- Scientific Computing and Data Management
- Phytoplasmas and Hemiptera pathogens
- Insect and Arachnid Ecology and Behavior
- Animal Behavior and Reproduction
- Probabilistic and Robust Engineering Design
- Molecular Biology Techniques and Applications
- Data Quality and Management
- Gene expression and cancer classification
National Institute of Biology
2012-2024
University of Ljubljana
1992-2015
Cardiff University
2014
Detection and quantification of plant pathogens in the presence inhibitory substances can be a challenge especially with environmental samples. Real-time quantitative PCR has enabled high-throughput detection pathogens; however, its use is linked to standardized reference materials, sensitivity inhibitors lead lower accuracy. Droplet digital been proposed as method overcome these drawbacks. Its absolute does not rely on standards tolerance demonstrated mostly clinical Such features would...
Extreme selective environments are commonly believed to funnel evolution toward a few predictable outcomes. Caves well‐known extreme with characteristically adapted faunas that similar in appearance, physiology, and behavior all over the world, even if not closely related. Morphological diversity between related cave species has been explained by difference time since colonization different ecological influence from surface. Here, we tested more classical hypothesis: morphological is...
Abstract Background Phytoplasmas are bacteria without cell walls from the class Mollicutes . They obligate intracellular plant pathogens which cause diseases in hundreds of economically important plants including grapevine ( Vitis vinifera ). Knowledge their biology and mechanisms interactions with hosts is largely unknown because they uncultivable experimentally inaccessible hosts. We detail here global transcriptional profiling responses to phytoplasmas. The gene expression patterns were...
Abstract The advantages of the digital PCR technology are already well documented until now. One way to achieve better cost efficiency technique is use it in a multiplexing strategy. Droplet platforms, which include two fluorescence filters, support at least duplex reactions and with some developments optimization higher possible. present study not only shows development multiplex assays droplet PCR, but also presents first thorough evaluation several parameters such PCR. Two 4-plex were...
The purpose of the investigation was to determine meanings attached by students different kinds molecular structure representations used in chemistry teaching. (n = 124) were from primary (aged 13-14 years) and secondary 17-18 schools a university 21-25 years). A computerised 'Chemical Visualisation Test' developed applied. research indicates that students' appreciation three-dimensional structures differs according kind representation used. best results achieved with use concrete,...
To investigate the dynamics of potato – Potato virus Y (PVY) compatible interaction in relation to salicylic acid - controlled pathways we performed experiments using non-transgenic cv. Désirée, transgenic NahG-Désirée, Igor and PVYNTN, most aggressive strain PVY. The importance viral multiplication symptom development was confirmed by pronounced depleted acid, reversion effect after spraying with 2,6-dichloroisonicotinic (a analogue). We have employed quantitative PCR for monitoring...
“If you know a thing only qualitatively, it no more than vaguely. If quantitatively—grasping some numerical measure that distinguishes from an infinite number of other possibilities—you are beginning to deeply.” (C. Sagan, Billions and Billions, 1997). Using
It has been suggested that both niche-based and neutral mechanisms are important for biological communities to evolve persist. For in extreme isolated environments such as caves, theoretical empirical considerations (low species turnover, high stress, strong convergence owing directional selection) predict functional equivalence of species. We tested this prediction using subterranean amphipod from caves interstitial groundwater. Contrary expectations, morphological diversity within habitats...
Potato virus Y (PVY) is a major pathogen that causes substantial economic losses in worldwide potato production. Different cultivars differ resistance to PVY, from severe susceptibility, through tolerance, complete resistance. The aim of this study was better define the mechanisms underlying tolerant responses infection by particularly aggressive PVYNTN strain. We focused on dynamics primary metabolism-related processes during infection. A comprehensive analysis dynamic changes metabolism...
SUMMARY Whereas the activation of resistance (R) proteins has been intensively studied, downstream signaling mechanisms leading to restriction pathogen remain mostly unknown. We studied immunity network response conditioned by potato Ny‐1 gene against virus Y. analyzed processes in cell death zone and surrounding tissue on biochemical expression levels order reveal spatiotemporal regulation immune response. show that transcriptional is dependent salicylic acid (SA). For some genes completely...
Abstract Potato (Solanum tuberosum) is highly water and space efficient but susceptible to abiotic stresses such as heat, drought, flooding, which are severely exacerbated by climate change. Our understanding of crop acclimation stress, however, remains limited. Here, we present a comprehensive molecular physiological high-throughput profiling potato tuberosum, cv. Désirée) under waterlogging applied single or in combinations designed mimic realistic future scenarios. Stress responses were...
In nature the aerial trace of pheromone used by male moths to find a female appears as train discontinuous pulses separated gaps among complex odorant background constituted plant volatiles. We investigated effect such odor on behavior and coding temporal parameters pulse trains in olfactory receptor neurons Spodoptera littoralis. Effects linalool were tested measuring walking towards source pheromone. While velocity orientation index did drop when was turned on, both recovered back...
Quantitative PCR (qPCR) is an important tool in pathogen detection. However, the use of different qPCR components, calibration materials and DNA extraction methods reduces comparability between laboratories, which can result false diagnosis discrepancies patient care. The wider establishment a metrological framework for nucleic acid tests could improve degree standardisation detection quantification applied clinical context. To achieve this, accurate need to be developed implemented as...
Our experiences shape our knowledge and understanding of the world around us. The natural vibrational environment (vibroscape) is hidden to human senses but nevertheless perceived exploited by majority animals. Here, we show that vibroscape recorded on plants in a temperate hay meadow dynamic low-frequency world, rich species-specific signals. overall composition changed throughout season also depended plant species, as well spatial position individual within meadow. Within studied...
Analysis of ecological data the arboricolous nephilid spider Clitaetra irenae Kuntner 2006, endemic to Maputaland forests, South Africa, indicates species' dependence on this highly threatened habitat. We tested C. habitat via GIS analysis by plotting known distribution against southern African ecoregions. In part its range, inhabits almost exclusively one ecoregion, coastal plain forests; but further north, in tropical it continues inland into Malawi's woodlands. test and refute hypotheses...
Background Glioblastoma multiforme (GBM) is among the most aggressive cancers with a poor prognosis in spite of plethora established diagnostic and prognostic biomarkers treatment modalities. Therefore, current goal detection novel biomarkers, possibly detectable blood GBM patients that may enable an early diagnosis are potential therapeutic targets, leading to more efficient interventions. Experimental Procedures MicroRNA profiling 734 human human-associated viral miRNAs was performed on...
The primary patterns of racial differentiation in the water louse, Asellus aquaticus (L.) sensu RACOVITZA, were assessed by analysis geographic variation 59 morphometric characters. Males from 20 localities Slovenia and 18 other countries analyzed. In Multivariate Discriminant Function Analysis, Minimum Spanning Tree Cluster samples comprising specimens without eye body pigmentation are separated with pigmented specimens, even though these two troglomorphic traits not being considered. Only...
Abstract Reliable delimitation and identification of species is central not only to systematics, but also studies biodiversity, ecology pest management. In the era I nternet‐based biodiversity databases misidentifications are rapidly disseminated may have far‐reaching consequences. Leafhoppers from genus Aphrodes ( H emiptera, C icadellidae) common abundant, but, nevertheless, they still a taxonomically challenging group whose members often assessed in ecological potential vectors plant...
Abstract Potato is highly water and space efficient but susceptible to abiotic stresses such as heat, drought, or flooding, which are severely exacerbated by climate change. Understanding of crop acclimation stress, however, remains limited. Here, we present a comprehensive molecular physiological high-throughput profiling potato ( Solanum tuberosum , cv. Desirée) under drought waterlogging applied single in combinations designed mimic realistic future scenarios. Stress-responses were...