A5007034867- Plant Virus Research Studies
- Alzheimer's disease research and treatments
- Bacteriophages and microbial interactions
- Trace Elements in Health
- Extracellular vesicles in disease
- Plant Pathogens and Resistance
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Plant responses to water stress
- Plant and Fungal Interactions Research
- Biochemical effects in animals
- Salmonella and Campylobacter epidemiology
- Plant Stress Responses and Tolerance
- Curcumin's Biomedical Applications
- Plant Disease Resistance and Genetics
- Mercury impact and mitigation studies
- Heavy Metal Exposure and Toxicity
- Transgenic Plants and Applications
- Listeria monocytogenes in Food Safety
- Virus-based gene therapy research
- Crustacean biology and ecology
- Phytoplasmas and Hemiptera pathogens
- Viral Infectious Diseases and Gene Expression in Insects
- Postharvest Quality and Shelf Life Management
- Insect and Arachnid Ecology and Behavior
National Institute of Biology
2016-2025
University of Ljubljana
2014-2016
Jožef Stefan Institute
2002-2012
One of the main challenges in gene therapy viral vector development is to establish an optimized process for its large scale production. This requires optimization upstream and downstream processes as well methods that enable step-by step analytical characterization virus, results which inform iterative refinement production yield, purity potency. The biggest problem here a plethora formulations, many interfere with techniques. We took adeno-associated virus (AAV) example showed benefits...
Structural and functional studies of the coat protein regions potato virus Y reveal crucial roles in viral infectivity.
ABSTRACT Abscission is a tightly regulated process in which plants shed unnecessary, infected, damaged, or aging organs, as well ripe fruits, through predetermined abscission zones response to developmental, hormonal, and environmental signals. Despite its importance, the underlying mechanisms remain incompletely understood. This study highlights deleterious effects of on chloroplast ultrastructure cells tomato flower pedicel zone, revealing spatiotemporal differential gene expression key...
Abstract Abscission occurs specifically in the abscission zone (AZ) tissue as a natural stage of plant development. Previously, we observed delay tomato (Solanum lycopersicum) leaf when LX ribonuclease (LX) was inhibited. The known association between expression and programmed cell death (PCD) suggested involvement PCD abscission. In this study, hallmarks were identified flower AZs during late These included loss viability, altered nuclear morphology, DNA fragmentation, elevated levels...
To define anti-Campylobacter jejuni activity of an extract from waste skins and seeds Pinot noir grapes (GSS), resveratrol possible resistance mechanisms, the influence these on Camp. morphology.Using gene-specific knock-out mutants efflux pump inhibitor, we showed CmeABC as most active for extrusion across outer membrane GSS resveratrol. Using polystyrene surface pig small intestine epithelial (PSI) human foetal (H4) cell lines, shows efficient inhibition adhesion to abiotic biotic...
Viruses represent one of the most important threats to agriculture. Several viral families include highly stable pathogens, which remain infective and can be transported long distances in water. The diversity plant viruses wastewater remains understudied; however, their potential impact is increasing with increased irrigation usage reclaimed wastewater. To determine abundance, biological relevance influents effluents we applied an optimized virus concentration method followed by...
To understand better the adaptation strategies of intra-annual radial growth in Pinus halepensis and sylvestris to local environmental conditions, we examined seasonal rhythm cambial activity cell differentiation at tissue cellular levels. Two contrasting sites differing temperature amount precipitation were selected for each species, one typical their other represented border climatic where two species coexisted. Mature P. trees from Mediterranean (Spain) sub-Mediterranean (Slovenia) sites,...
SUMMARY Whereas the activation of resistance (R) proteins has been intensively studied, downstream signaling mechanisms leading to restriction pathogen remain mostly unknown. We studied immunity network response conditioned by potato Ny‐1 gene against virus Y. analyzed processes in cell death zone and surrounding tissue on biochemical expression levels order reveal spatiotemporal regulation immune response. show that transcriptional is dependent salicylic acid (SA). For some genes completely...
Water scarcity is one of the greatest threats for human survival and quality life, this increasingly contributing to risk human, animal plant infections due waterborne viruses. Viruses are transmitted through polluted water, where they can survive cause even at low concentrations. Plant viruses from genus Tobamovirus highly mechanically transmissible, considerable damage important crops, such as tomato. The release infective tobamoviruses into environmental waters has been reported, with...
Abstract The structural diversity and tunability of the capsid proteins (CPs) various icosahedral rod-shaped viruses have been well studied exploited in development smart hybrid nanoparticles. However, potential CPs wide-spread flexuous filamentous plant remains to be explored. Here, we show that can control shape, size, RNA encapsidation ability, symmetry, stability surface functionalization nanoparticles through structure-based design CP from potato virus Y (PVY). We provide...
The distribution of Potato virus Y (PVY) in the systemically infected potato (Solanum tuberosum) plants highly susceptible cultivar Igor was investigated. Virus presence and accumulation analyzed different plant organs tissues using real-time polymerase chain reaction transmission electron microscopy (TEM) negative staining methods. To get a complete insight into location viral RNA within tissue, situ hybridization developed optimized for detection PVY at cellular level. shown to accumulate...
Extracellular vesicle-bound DNA (evDNA) is an understudied extracellular vesicle (EV) cargo, particularly in cancer-unrelated research. Although evDNA has been detected urine, little known about its characteristics, localization, and biomarker potential for kidney pathologies. To address this, we enriched EVs from urine of well-characterized transplant recipients undergoing allograft biopsy, characterized their association to injury. The SEC-based method pure recipients, regardless the...
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Amyloid fibril formation is a shared property of all proteins; therefore, model proteins can be used to study this process. We measured protein aggregation the amyloid‐forming stefin B in presence and absence several antioxidants. by was routinely induced at pH 5 10% TFE, room temperature. The effects antioxidants NAC, vitamin C, E, three polyphenols resveratrol, quercetin, curcumin on kinetics were followed using ThT fluorescence. Concomitantly, morphology amount aggregates fibrils checked...
Ethylene has impact on several physiological plant processes, including abscission, during which plants shed both their vegetative and reproductive organs. Cell separation programmed cell death are involved in these have also been correlated with ethylene action. However, the detailed spatiotemporal pattern of molecular events abscission remains unknown. We examined expression two tomato ACO genes, LeACO1 LeACO4 that encode last enzyme biosynthesis, 1-aminocyclopropane-1-carboxylate oxidase...
Ring-opening polymerization of<italic>N</italic>-carboxyanhydrides was performed in oil-in-oil high internal phase emulsion to obtain well-defined macroporous synthetic polypeptides.
Recombinant adeno-associated viruses (rAAVs) play a pivotal role in the treatment of genetic diseases. However, current production and purification processes yield AAV-based preparations that often contain unwanted empty, partially filled or damaged viral particles impurities, including residual host cell DNA proteins, plasmid DNA, aggregates. To precisely understand composition AAV preparations, we systematically compared four different single-stranded (ssAAV) self-complementary (scAAV)...
We show that human stefin B, a protease inhibitor from the family of cystatins, is copper binding protein, unlike A. have used isothermal titration calorimetry to directly monitor event at pH 7 and 5. At B shows picomolar affinity for but 5 in nanomolar range. There no difference between wildtype (E31 isoform) variant (Y31 isoform), whereas mutant (P79S), which tetrameric, does not bind copper. The conformation remains unaltered by binding. It known below undergoes conformational change...
The human sperm head vacuoles and their role in male infertility are still poorly understood. aim of this study was to identify the clinical ultrastructural features men included vitro fertilization programme: with normal (normozoospermia) impaired morphology (teratozoospermia). samples were observed under 6000-time magnification using motile organelle examination (MSOME). proportion evaluated related outcome fertilization. characterized by a higher vacuoles. semen quality (sperm...
The outbreak of the worrisome coronavirus disease in 2019 has caused great concern among global public, especially regarding need for personal protective equipment with applied antiviral agents to reduce spread and transmission virus. Thus, our research, chitosan-based bioactive polymers as potential were first evaluated colloidal macromolecular solutions by elemental analysis charge. Three different types low high molecular weight chitosan (LMW Ch, HMW Ch) a LMW Ch derivative, i.e.,...
Ralstonia solanaceraum is the quarantine plant pathogenic bacterium that causes bacterial wilt in over 200 host plants, which include economically important crops such as potato, tomato, tobacco, banana, and ginger. Alternative biological methods of disease control can be used integrated pest management are extensively studied. In search new proteins with antibacterial activity against R. solanacearum, we identified L-amino acid oxidases (LAOs) from fruiting bodies Amanita phalloides (ApLAO)...
Oligomers are commonly observed intermediates at the initial stages of amyloid fibril formation. They toxic to neurons and cause decrease in neural transmission long-term potentiation. We describe an vitro study steps formation by human stefin B, which proved be a good model system. Due relative stability oligomers electrospray ionization mass spectrometry (ESI MS) could applied addition size exclusion chromatography (SEC). These two techniques enabled us separate detect distinguished from...